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Gene Review

trp  -  transient receptor potential

Drosophila melanogaster

Synonyms: 559, CG7875, DmTRP, Dmel\CG7875, TRP, ...
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Disease relevance of trp


High impact information on trp

  • TRP channel proteins and signal transduction [2].
  • TRP-related channel proteins are found in a variety of organisms, tissues, and cell types, including nonexcitable, smooth muscle, and neuronal cells [2].
  • The molecular domains that are conserved in all members of the TRP family constitute parts of the transmembrane domains and in most members also the ankyrin-like repeats at the NH2 terminal of the protein and a "TRP domain" at the COOH terminal, which is a highly conserved 25-amino acid stretch with still unknown function [2].
  • We propose that the light-activated current in photoreceptor cells is produced by a combination of TRP homo- and TRP-TRPL heteromultimers [4].
  • Contrary to current models of excitation and TRP channel function, we demonstrate that the transient phenotype of trp mutants can be explained by CAM regulation of the TRPL channel rather than by the loss of a store-operated conductance leading to depletion of the internal stores [5].

Chemical compound and disease context of trp

  • This retinal degeneration was greatly suppressed by elimination of the Na(+)/Ca(2+) exchanger, CalX, indicating that the cell death was due primarily to deficient Ca(2+) entry rather than disruption of the TRP-anchoring function [1].

Biological context of trp


Anatomical context of trp

  • Abolishing Ca2+ influx in wild-type photoreceptors mimicked inactivation, while raising Ca2+ by blocking Na+/Ca2+ exchange prevented inactivation in trp [11].
  • It is not clear how the Ca2+ store depletion signal is relayed to the plasma membrane and whether both TRP and TRPL participate in this process [12].
  • Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry [12].
  • Transient receptor potential (TRP) channels across species are expressed in sensory receptor cells, and often localized to specialized subcellular sites [13].
  • At least seven proteins bind INAD, including the transient receptor potential (TRP) channel, which depends on INAD for localization to the phototransducing organelle, the rhabdomere [14].

Associations of trp with chemical compounds


Physical interactions of trp

  • We report that anti-INAD antibodies coimmunoprecipitate TRP, identified by its electrophoretic mobility, cross reactivity with anti-TRP antibody, and absence in a null allele trp mutant [17].
  • Furthermore, Rab11 is required for transport of TRP, another rhabdomeric protein, and for development of specialized membrane structures within Garland cells [18].

Enzymatic interactions of trp

  • We reveal that Ser982 of TRP is phosphorylated by eye-PKC in vitro and, importantly, in the fly eye, as shown by mass spectrometry [19].

Regulatory relationships of trp

  • These results suggest that the INAD macromolecular complex plays an essential role in termination of the light response by promoting efficient phosphorylation at Ser982 of TRP for fast deactivation of the visual signaling [19].
  • The open channel block by Ca(2+) that we characterized is a useful mechanism to improve the signal to noise ratio of the response to intense light when virtually all the large conductance TRPL channels are blocked and only the low conductance TRP channels with lower Ca(2+) affinity are active [20].
  • Mutations in calnexin also impair the ability of photoreceptor cells to control cytosolic Ca2+ levels following activation of the light-sensitive TRP channels [21].

Other interactions of trp

  • The Drosophila light-activated conductance is composed of the two channels TRP and TRPL [22].
  • Use of antipeptide polyclonal antibodies for TRP, TRPL, and TRPgamma showed expression of all three channels in type 1 (principal) cells in the tubule main segment [6].
  • Moreover, deactivation is regulated by the interaction between INAD and TRP, because abrogation of this interaction in InaD(p215) results in slow deactivation similar to that of inaC(p209) lacking eye-PKC [19].
  • Together our results demonstrate the contribution of PI depletion to the rdgA phenotype and provide evidence that depletion of PI and its metabolites might be a key signal for TRP channel activation in vivo [23].
  • Here, we report on the Drosophila inaF gene, which encodes a highly eye-enriched protein, INAF, that appears to be required for TRP channel function [24].

Analytical, diagnostic and therapeutic context of trp


  1. Dissecting independent channel and scaffolding roles of the Drosophila transient receptor potential channel. Wang, T., Jiao, Y., Montell, C. J. Cell Biol. (2005) [Pubmed]
  2. TRP channel proteins and signal transduction. Minke, B., Cook, B. Physiol. Rev. (2002) [Pubmed]
  3. Emerging functions of 10 types of TRP cationic channel in vascular smooth muscle. Beech, D.J. Clin. Exp. Pharmacol. Physiol. (2005) [Pubmed]
  4. Coassembly of TRP and TRPL produces a distinct store-operated conductance. Xu, X.Z., Li, H.S., Guggino, W.B., Montell, C. Cell (1997) [Pubmed]
  5. Calmodulin regulation of Drosophila light-activated channels and receptor function mediates termination of the light response in vivo. Scott, K., Sun, Y., Beckingham, K., Zuker, C.S. Cell (1997) [Pubmed]
  6. Transient receptor potential-like channels are essential for calcium signaling and fluid transport in a Drosophila epithelium. MacPherson, M.R., Pollock, V.P., Kean, L., Southall, T.D., Giannakou, M.E., Broderick, K.E., Dow, J.A., Hardie, R.C., Davies, S.A. Genetics (2005) [Pubmed]
  7. Insights on trp channels from in vivo studies in Drosophila. Minke, B., Parnas, M. Annu. Rev. Physiol. (2006) [Pubmed]
  8. Phenotypes of trpl mutants and interactions between the transient receptor potential (TRP) and TRP-like channels in Drosophila. Leung, H.T., Geng, C., Pak, W.L. J. Neurosci. (2000) [Pubmed]
  9. Reversible phosphorylation of the signal transduction complex in Drosophila photoreceptors. Liu, M., Parker, L.L., Wadzinski, B.E., Shieh, B.H. J. Biol. Chem. (2000) [Pubmed]
  10. Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade. Meyer, N.E., Joel-Almagor, T., Frechter, S., Minke, B., Huber, A. J. Cell. Sci. (2006) [Pubmed]
  11. Calcium influx via TRP channels is required to maintain PIP2 levels in Drosophila photoreceptors. Hardie, R.C., Raghu, P., Moore, S., Juusola, M., Baines, R.A., Sweeney, S.T. Neuron (2001) [Pubmed]
  12. Coexpression of Drosophila TRP and TRP-like proteins in Xenopus oocytes reconstitutes capacitative Ca2+ entry. Gillo, B., Chorna, I., Cohen, H., Cook, B., Manistersky, I., Chorev, M., Arnon, A., Pollock, J.A., Selinger, Z., Minke, B. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  13. Two stages of light-dependent TRPL-channel translocation in Drosophila photoreceptors. Cronin, M.A., Lieu, M.H., Tsunoda, S. J. Cell. Sci. (2006) [Pubmed]
  14. TRP and the PDZ protein, INAD, form the core complex required for retention of the signalplex in Drosophila photoreceptor cells. Li, H.S., Montell, C. J. Cell Biol. (2000) [Pubmed]
  15. Activation of the Drosophila TRP and TRPL channels requires both Ca2+ and protein dephosphorylation. Agam, K., Frechter, S., Minke, B. Cell Calcium (2004) [Pubmed]
  16. TRP channels in Drosophila photoreceptors: the lipid connection. Hardie, R.C. Cell Calcium (2003) [Pubmed]
  17. Regulation of the TRP Ca2+ channel by INAD in Drosophila photoreceptors. Shieh, B.H., Zhu, M.Y. Neuron (1996) [Pubmed]
  18. Rab11 mediates post-Golgi trafficking of rhodopsin to the photosensitive apical membrane of Drosophila photoreceptors. Satoh, A.K., O'Tousa, J.E., Ozaki, K., Ready, D.F. Development (2005) [Pubmed]
  19. Scaffolding protein INAD regulates deactivation of vision by promoting phosphorylation of transient receptor potential by eye protein kinase C in Drosophila. Popescu, D.C., Ham, A.J., Shieh, B.H. J. Neurosci. (2006) [Pubmed]
  20. Open Channel Block by Ca2+ Underlies the Voltage Dependence of Drosophila TRPL Channel. Parnas, M., Katz, B., Minke, B. J. Gen. Physiol. (2007) [Pubmed]
  21. Calnexin is essential for rhodopsin maturation, Ca2+ regulation, and photoreceptor cell survival. Rosenbaum, E.E., Hardie, R.C., Colley, N.J. Neuron (2006) [Pubmed]
  22. The Drosophila light-activated conductance is composed of the two channels TRP and TRPL. Niemeyer, B.A., Suzuki, E., Scott, K., Jalink, K., Zuker, C.S. Cell (1996) [Pubmed]
  23. lazaro encodes a lipid phosphate phosphohydrolase that regulates phosphatidylinositol turnover during Drosophila phototransduction. Garcia-Murillas, I., Pettitt, T., Macdonald, E., Okkenhaug, H., Georgiev, P., Trivedi, D., Hassan, B., Wakelam, M., Raghu, P. Neuron (2006) [Pubmed]
  24. INAF, a protein required for transient receptor potential Ca(2+) channel function. Li, C., Geng, C., Leung, H.T., Hong, Y.S., Strong, L.L., Schneuwly, S., Pak, W.L. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  25. Constitutive activity of the light-sensitive channels TRP and TRPL in the Drosophila diacylglycerol kinase mutant, rdgA. Raghu, P., Usher, K., Jonas, S., Chyb, S., Polyanovsky, A., Hardie, R.C. Neuron (2000) [Pubmed]
  26. Metabolic stress reversibly activates the Drosophila light-sensitive channels TRP and TRPL in vivo. Agam, K., von Campenhausen, M., Levy, S., Ben-Ami, H.C., Cook, B., Kirschfeld, K., Minke, B. J. Neurosci. (2000) [Pubmed]
  27. Calcium homeostasis in photoreceptor cells of Drosophila mutants inaC and trp studied with the pupil mechanism. Hofstee, C.A., Stavenga, D.G. Vis. Neurosci. (1996) [Pubmed]
  28. Isolation of genes encoding photoreceptor-specific proteins by immunoscreening with antibodies directed against purified blowfly rhabdoms. Huber, A., Sander, P., Wolfrum, U., Groell, C., Gerdon, G., Paulsen, R. J. Photochem. Photobiol. B, Biol. (1996) [Pubmed]
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