High impact information on Microspectrophotometry

• To confirm the physiological relevance of the structural findings, we conducted single-crystal microspectrophotometry on rhodopsin packed in our three-dimensional crystals and show that its spectroscopic properties are similar to those previously found by using bovine rhodopsin in suspension or membrane environment [1].
• Microscopy and microspectrophotometry demonstrated that plaque from beta carotene-treated patients had higher carotenoid levels and higher absorption (450-500 nm) compared with control specimens, but normal media was unaffected [2].
• We have analyzed the frequency and distribution of cells expressing estrogen receptor (ER) in cryosections of normal human breast tissue using quantitative microspectrophotometry and the estrogen receptor immunocytochemical assay [3].
• Hepatocytes contributing to the uptake, metabolism and biliary secretion of BSP were directly assessed qualitatively by light microscopy, and also semiquantitatively by microspectrophotometry [4].
• At the squid giant synapse, a presynaptic action potential is accompanied by an influx of calcium ions that continue to be detectable with arsenazo III microspectrophotometry for several seconds [5].

Biological context of Microspectrophotometry

• The technique of combined Feulgen microspectrophotometry and 3H-thymidine autoradiography demonstrated a pile-up of early polychromatic erythroblasts in the G1 and G2 phases of the cell cycle, indicating a prolongation of, or an arrest at, these phases [6].
• Natural substrates and analogs rapidly diffuse through crystals of pig heart mitochondrial aspartate aminotransferase and react at the active sites causing spectral changes that can be measured by single-crystal microspectrophotometry [7].
• Regional myocardial oxygen consumption was calculated from O2 extraction using microspectrophotometry and blood flow using radioactively labeled microspheres [8].
• Coronary blood flow (radioactive microspheres), arterial and venous O2 saturation (microspectrophotometry), O2 consumption, cyclic GMP content (competitive binding) and cyclic GMP-phosphodiesterase activity (conversion of 3H-cyclic GMP to 3H-GMP) were determined [9].
• Coronary blood flow and cardiac output, using radioactive microspheres, and small vessel O2 saturations, using microspectrophotometry, were measured in hypertrophied and nonhypertrophied hearts [10].

Anatomical context of Microspectrophotometry

• We conducted a detailed, area-specific analysis, measuring the thickness of articular cartilage and analyzing the distribution of Safranin O stain that binds stoichiometrically to GAG as determined by quantitative microspectrophotometry [11].
• Optical microspectrophotometry of Laurdan-labeled neutrophils revealed a large blue shift at lamellipodia relative to cell bodies [12].
• The time-dependent release of superoxide anion (O2-) from bradykinin (Bk)-stimulated human umbilical vein endothelial cells (EC) was measured as the superoxide dismutase-inhibitable reduction of ferricytochrome C employing a novel application of microspectrophotometry [13].
• Enzyme activity was measured by microspectrophotometry in individual Leydig cells in cryostat sections of fetal testes on Days 16-21 postconception [14].
• The expression of an oestrogen receptor-related antigen has been measured by microspectrophotometry in the glandular epithelium and stroma of tissue sections from endometrial biopsies of women administered exogenous replacement of sex steroids because of premature ovarian failure [15].

Associations of Microspectrophotometry with chemical compounds

• End-point analyses included macroscopic joint examination, immuno- and TUNEL staining, Safranin O staining/microspectrophotometry, and tumor necrosis factor alpha (TNFalpha) convertase enzyme (TACE) inhibition assay [16].
• Catalytic activity in crystals of mitochondrial aspartate aminotransferase as detected by microspectrophotometry [17].
• We have investigated by single crystal microspectrophotometry the functional properties of a mutant lacking the S-adenosylmethionine binding domain [18].
• Kinetic studies of crystalline enzymes by single crystal microspectrophotometry. Analysis of a single catalytic turnover in a D-glyceraldehyde-3-phosphate dehydrogenase crystal [19].
• The reactivity of GABA-AT crystals with the two GABA analogues was also investigated by polarized absorption microspectrophotometry [20].

Gene context of Microspectrophotometry

• The light-driven pupil mechanism, consisting of an assembly of mobile pigment granules inside the photoreceptor cells, has been investigated by in vivo reflection microspectrophotometry in wild type (WT) Drosophila and in the photoreceptor mutants inaC and trp [21].
• Microspectrophotometry was used to compare the rhodopsin concentrations in wild-type flies and norpA vision transduction mutants [22].
• Microspectrophotometry of rhodopsin and metarhodopsin in the moth Galleria [23].
• METHODS: Microspectrophotometry (MSP) was used to assess rhodopsin absorbances in localized regions of isolated ROS from dark-adapted 13-, 19-, and 34-day-old and adult rats [24].
• The same individual cells were first measured for T antigen and then for DNA by Feulgen microspectrophotometry [25].

Analytical, diagnostic and therapeutic context of Microspectrophotometry

• Small artery and vein O2 saturations (microspectrophotometry) and regional flow (radioactive microspheres) were determined [26].
• This is supported by the measurement of DNA synthesis by autoradiography and measurement of cellular DNA by two methods--the diphenylamine reaction and microspectrophotometry of Feulgen stained cells [27].
• Here we used microspectrophotometry and in situ hybridization with cDNA probes to study the distribution of UV and blue cones in the retinas of four species of Pacific salmon (coho, chum, chinook, and pink salmon), in the Atlantic salmon, and in the rainbow/steelhead trout [28].
• Another 14 ovariectomized rats were separated into 17beta-estradiol and control groups to determine cerebral blood flow (14C-iodoantipyrine) and O(2) consumption (microspectrophotometry) [29].
• Reduction mechanism of L-cysteine on keratin fibers using microspectrophotometry and Raman spectroscopy [30].

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