Disease relevance of HNT

• Consistent with these results, Ntm-Fc binds to neurons that express Ntm at high levels, e.g., dorsal root ganglion (DRG) and hippocampal neurons [1].
• In a pit formation assay, addition of conditioned medium (CM) derived from BHY but not HNT increased osteoclastic bone resorption, and the effects were inhibited by anti-IL-6 antibody [2].
• RESULTS: HNT neuron transplantation in the SOD1 mice delayed disease progression for 3-4 weeks, although lifespan was not affected [3].
• RESULTS: BHY, a bone-invasive oral cancer cell line, but not HNT, a noninvasive cell line, produced large amounts of IL-6 [2].
• It has been reported that the C-terminus of the second conserved region (C2) of the envelope glycoprotein gp120, encompassing peptide RSANFTDNAKTIIVQLNESVEIN (NTM), is important for infectivity and neutralization of the human immunodeficiency virus type 1 (HIV-1) [4].

High impact information on HNT

• Neurotrimin mediates bifunctional effects on neurite outgrowth via homophilic and heterophilic interactions [1].
• The second method involved pre-addition of HNT to membranes carrying the precursive C5b-7 complex and subsequent conversion to MC5b-8 and MC5b-9 by addition of exogenous C8 and C9 [5].
• Because labeling by HNT is specific for intramembrane structural domains of proteins, these results provide direct evidence that constituents of terminal complement complexes insert into the lipid bilayer of cell membranes during lysis [5].
• Radioiodinated hexanoyldiiodo-N-(4-azido-2-nitrophenyl)tyramine (HNT) was employed as a photosensitive membrane-restricted probe to establish whether terminal complement proteins insert into membrane bilayers during cytolysis [5].
• Previous work has shown that limbic-system-associated protein (LAMP), CEPU-1/Neurotrimin and OBCAM interact homophilically and heterophilically within the family [6].

Biological context of HNT

• Solution pH had a dramatic effect on the kinetics of apoE3 NT-mediated Myr2Gro-PCho vesicle transformation with increased rates observed as a function of decreasing pH [7].
• Changes in blood volume (BV) during dialysis as well as plasma levels of brain natriuretic peptide (BNP) and N-terminal (NT) pro-BNP levels are possible tools to assess dry weight in hemodialysis (HD) patients [8].

Anatomical context of HNT

• The pcDNA3.1-HA-NTM is transfected into the Chinese Hamster Ovary (CHO) cells [9].
• In conclusion, oCRF is a potent secretagogue for the equimolar release of ACTH, HNT and related peptides in the human pituitary gland [10].
• The fifth line (HNT in vero cells) did not produce any infectious virus and only a fraction of the cells were infected in most passages [11].
• Patients were separated into three anatomic groups: head, neck, or trunk (HNT); lower extremity (LE); and upper extremity (UE) [12].
• A cervical biopsy is not to be performed until a complete HNT examination (including the thyroid and major salivary glands) has excluded the presence of a primary tumor in these regions [13].

Associations of HNT with chemical compounds

• CEPU-1/Neurotrimin is a neuronal glycoprotein thought to play a role in axon guidance and cell-cell recognition [14].
• Exposure to phytohemagglutinin (PHA) stimulated glycolytic metabolism in T-cells but did not stimulate glucose utilization or lactate production in NTM [15].
• 0. Similarly, fluorescent dye binding experiments with 8-anilino-1-naphthalene sulfonate revealed increased exposure of apoE3 NT hydrophobic interior as a function of decreasing pH [7].
• Fluorescence studies with a single tryptophan containing apoE3 NT mutant (L155W) revealed increased solvent exposure of the protein interior at pH values below 4 [7].

Other interactions of HNT

• These data indicate that LAMP is a highly conserved new member of the IgSF which, together with the opioid-binding cell adhesion molecule (OBCAM) and neurotrimin, comprises a new subfamily that has been designated as IgLONs [16].
• The family consists of LAMP, OBCAM, neurotrimin/CEPU-1 and neurotractin/kilon [17].
• RESULTS: IMT was significantly higher in HTN than in C (0.6 +/- 0.1 vs 0.4 +/- 0.1 mm, p < 0.001) as well as LVMI (119.5 +/- 39.9 vs 106.8+/-18.7 g/m2, p = 0.04) and serum TIMP-1 (in HNT 691.7 +/- 124.6 ng/ml; in C 577.5+/-70.8 ng/ml, p < 0.001) [18].

Analytical, diagnostic and therapeutic context of HNT

• Those constituents labeled by HNT within each complex were identified by autoradiography [5].
• The decline in BV during regular dialysis and during an ultrafiltration pulse, as well as VCDI and BNP were significantly different between the tertiles of normalized TBW, but refill after the ultrafiltration pulse and NT pro-BNP were not [8].

References