The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Mad2l1  -  MAD2 mitotic arrest deficient-like 1

Mus musculus

Synonyms: AA673185, MAD2, MAD2-like protein 1, Mad2a, Mitotic arrest deficient 2-like protein 1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on Mad2l1


Biological context of Mad2l1

  • Consistently, reconstitution of Mad2 in Brca1(Delta11/Delta11) cells partially restored the spindle checkpoint and attenuated apoptosis [3].
  • The end of prometaphase is correlated with checkpoint inactivation and disappearance of MAD2L1 (MAD2) and RSN (CLIP-170) proteins from kinetochores [4].
  • Disruption of Mad2, BubR1, Bub3 or Rae1 in mice results in substantial aneuploidy in somatic tissues, but whether these genes are equally important for accurate chromosome segregation during meiosis has not yet been established [5].
  • We conclude that the mitotic checkpoint is not essential for viability per se and that a CIN phenotype can be established in culture through the inactivation of both the Mad2- and p53-dependent checkpoint pathways [6].
  • Second, the absolute rate of chromosome missegregation may be increased by alterations in the levels of two proteins, separase and Mad2, which are important for maintaining chromosomal segregation and the normal spindle checkpoint during mitosis [7].

Anatomical context of Mad2l1

  • Our study shows for the first time that a functional Mad2-dependent spindle checkpoint exists during the first meiotic division in mammalian oocytes [8].
  • In contrast, the microtubule stabilizer taxol induced the loss of Mad2 from the majority of the first-division-metaphase kinetochores in which it was normally present in untreated cells [9].
  • Double labelling immunofluorescence with MAD-2 and a mouse monoclonal antibody against vimentin, the intermediate filament protein of cells of mesenchymal origin, showed coincidental staining which was distinct from that seen with antibodies against other cytoskeletal proteins [10].

Associations of Mad2l1 with chemical compounds


Co-localisations of Mad2l1


Other interactions of Mad2l1

  • Similar results were obtained using dominant-negative forms of Mad2 and BubR1, as well as checkpoint resistant dominant APC/C activating forms of Cdc20 [14].
  • Generating chromosome instability through the simultaneous deletion of Mad2 and p53 [6].

Analytical, diagnostic and therapeutic context of Mad2l1


  1. Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2. Dobles, M., Liberal, V., Scott, M.L., Benezra, R., Sorger, P.K. Cell (2000) [Pubmed]
  2. MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. Michel, L.S., Liberal, V., Chatterjee, A., Kirchwegger, R., Pasche, B., Gerald, W., Dobles, M., Sorger, P.K., Murty, V.V., Benezra, R. Nature (2001) [Pubmed]
  3. A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint. Wang, R.H., Yu, H., Deng, C.X. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  4. The first mitosis of the mouse embryo is prolonged by transitional metaphase arrest. Sikora-Polaczek, M., Hupalowska, A., Polanski, Z., Kubiak, J.Z., Ciemerych, M.A. Biol. Reprod. (2006) [Pubmed]
  5. Differential mitotic checkpoint protein requirements in somatic and germ cells. Jeganathan, K.B., van Deursen, J.M. Biochem. Soc. Trans. (2006) [Pubmed]
  6. Generating chromosome instability through the simultaneous deletion of Mad2 and p53. Burds, A.A., Lutum, A.S., Sorger, P.K. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  7. Hormone-induced chromosomal instability in p53-null mammary epithelium. Pati, D., Haddad, B.R., Haegele, A., Thompson, H., Kittrell, F.S., Shepard, A., Montagna, C., Zhang, N., Ge, G., Otta, S.K., McCarthy, M., Ullrich, R.L., Medina, D. Cancer Res. (2004) [Pubmed]
  8. Metaphase I arrest upon activation of the Mad2-dependent spindle checkpoint in mouse oocytes. Wassmann, K., Niault, T., Maro, B. Curr. Biol. (2003) [Pubmed]
  9. Differences in spindle association of the mitotic checkpoint protein Mad2 in mammalian spermatogenesis and oogenesis. Kallio, M., Eriksson, J.E., Gorbsky, G.J. Dev. Biol. (2000) [Pubmed]
  10. Reactivity of a human monoclonal antibody against Rh D with the intermediate filament protein vimentin. Thorpe, S.J. Br. J. Haematol. (1990) [Pubmed]
  11. Monoclonal antibodies against doxorubicin. Balsari, A., Alzani, R., Parrello, D., Morelli, D., Tagliabue, E., Gianni, L., Isetta, A.M., Menard, S., Colnaghi, M.I., Ghione, M. Int. J. Cancer (1988) [Pubmed]
  12. Global gene profiling analysis of mouse uterus during the oestrous cycle. Tan, Y.F., Li, F.X., Piao, Y.S., Sun, X.Y., Wang, Y.L. Reproduction (2003) [Pubmed]
  13. Mad2 is required for optimal hematopoiesis: Mad2 associates with c-Kit in MO7e cells. Ito, S., Mantel, C.R., Han, M.K., Basu, S., Fukuda, S., Cooper, S., Broxmeyer, H.E. Blood (2007) [Pubmed]
  14. The spindle assembly checkpoint is not essential for CSF arrest of mouse oocytes. Tsurumi, C., Hoffmann, S., Geley, S., Graeser, R., Polanski, Z. J. Cell Biol. (2004) [Pubmed]
  15. RNA Interference as a tool to study the function of MAD2 in mouse oocyte meiotic maturation. Wang, J.Y., Lei, Z.L., Nan, C.L., Yin, S., Liu, J., Hou, Y., Li, Y.L., Chen, D.Y., Sun, Q.Y. Mol. Reprod. Dev. (2007) [Pubmed]
WikiGenes - Universities