The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

Nsfl1c  -  NSFL1 (p97) cofactor (p47)

Rattus norvegicus

Synonyms: NSFL1 cofactor p47, XY body-associated protein XY40, XY40, p47, p97 cofactor p47
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on Nsfl1c

  • The finding of a ubiquitin-binding domain in p47 raises the question as to whether the ubiquitin-proteasome system is also involved in membrane fusion events [1].
  • Together with its adaptor p47, on the other hand, it regulates several membrane fusion events, including reassembly of Golgi cisternae after mitosis [1].
  • Fractionation of cytosol revealed that p97 and its co-factor, p47, constitutes the major fusion activity [2].
  • Recruitment of the p47-containing complex onto cell membranes is stimulated by GTP gamma S and blocked by brefeldin A, indicating that, like other coat proteins, its membrane association is regulated by an ARF [3].
  • However, p47 is not enriched in preparations of clathrin-coated vesicles [3].

Biological context of Nsfl1c

  • We speculate that protein XY40 may be involved in the allocyclic behavior of sex chromosomes during male meiotic prophase [4].
  • In contrast to p47, however, Ufd1-Npl4 does not regulate the ATPase activity of p97; nor does a variant of p47 that contains both binding sites but lacks the N-terminal domains [5].
  • Importantly, PE-mediated changes in secondary and tertiary structures are exclusively observed when p97 is complexed with p47, which is a prerequisite for membrane fusion [6].
  • The ATPase p97 in complex with p47 participates in Golgi cisternae rebuilding after mitosis [6].
  • The nucleic acid sequence reveals that XY40 is a novel protein with a few similarities to already known nucleotide sequences [7].

Anatomical context of Nsfl1c

  • Biochemical characterization showed that protein XY40 (40 kDa; pI 5.7-5.8) can be extracted from rat pachytene spermatocytes and recovered in particles of 9.5 S with a native molecular mass of approximately 152 kDa [4].
  • The ATPase associated with different cellular activities family member p97, associated p47, and the t-SNARE syntaxin 5 are necessary for the cell-free reconstitution of transitional endoplasmic reticulum (tER) from starting low-density microsomes [8].
  • Immunocytochemical localization on rat testis cryosections with a XY40-specific monoclonal antibody revealed that the labeling is confined to the axial elements of sex chromosomes [4].
  • Phospholipid species act as modulators in p97/p47-mediated fusion of Golgi membranes [6].
  • Here we show for the first time that fusion between mitotic Golgi membranes induced by adding cytosol or purified p97/p47 is modulated by PE present in Golgi membranes [6].

Associations of Nsfl1c with chemical compounds

  • Like the ubiquitin fold ubiquitin regulatory X (UBX) domain in p47, the Npl4-UBD interacts with p97 via the loop between its strands 3 and 4 and a conserved arginine in strand 1 [5].
  • We therefore propose that at physiological conditions PE-induced conformational changes in p97/p47 are relevant in triggering this activity [6].
  • Previously it had been shown that p97-mediated reassembly of Golgi cisternae from mitotic Golgi fragments requires p47 which mediates the binding of p97 to a Golgi t-SNARE (soluble N-ethylmaleimide-sensitive factor attachment factor receptor), syntaxin 5 [9].
  • RESULTS: Incubation for 24 h in 0.1 mmol/l palmitic acid or a pro-inflammatory cytokine cocktail increased p47( phox ) protein production by 1.5-fold or by 1.75-fold, respectively, in the BRIN BD11 beta cell line [10].
  • AIMS/HYPOTHESIS: Acute or chronic exposure of beta cells to glucose, palmitic acid or pro-inflammatory cytokines will result in increased production of the p47( phox ) component of the NADPH oxidase and subsequent production of reactive oxygen species (ROS) [10].

Other interactions of Nsfl1c

  • The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism [5].
  • Since the transcripts of both XY40 and Munc18-c show a similar expression pattern, it is tempting to speculate that this common sequence is involved in translation regulation [7].

Analytical, diagnostic and therapeutic context of Nsfl1c


  1. VCIP135 acts as a deubiquitinating enzyme during p97-p47-mediated reassembly of mitotic Golgi fragments. Wang, Y., Satoh, A., Warren, G., Meyer, H.H., Wang, Y. J. Cell Biol. (2004) [Pubmed]
  2. Cytosolic ATPases, p97 and NSF, are sufficient to mediate rapid membrane fusion. Otter-Nilsson, M., Hendriks, R., Pecheur-Huet, E.I., Hoekstra, D., Nilsson, T. EMBO J. (1999) [Pubmed]
  3. A novel adaptor-related protein complex. Simpson, F., Bright, N.A., West, M.A., Newman, L.S., Darnell, R.B., Robinson, M.S. J. Cell Biol. (1996) [Pubmed]
  4. Meiosis-specific protein selectively associated with sex chromosomes of rat pachytene spermatocytes. Smith, A., Benavente, R. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  5. The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism. Bruderer, R.M., Brasseur, C., Meyer, H.H. J. Biol. Chem. (2004) [Pubmed]
  6. Phospholipid species act as modulators in p97/p47-mediated fusion of Golgi membranes. Pécheur, E.I., Martin, I., Maier, O., Bakowsky, U., Ruysschaert, J.M., Hoekstra, D. Biochemistry (2002) [Pubmed]
  7. Molecular characterization and expression pattern of XY body-associated protein XY40 of the rat. Alsheimer, M., Imamichi, Y., Heid, H., Benavente, R. Chromosoma (1997) [Pubmed]
  8. Tyrosine phosphorylation of p97 regulates transitional endoplasmic reticulum assembly in vitro. Lavoie, C., Chevet, E., Roy, L., Tonks, N.K., Fazel, A., Posner, B.I., Paiement, J., Bergeron, J.J. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  9. The p47 co-factor regulates the ATPase activity of the membrane fusion protein, p97. Meyer, H.H., Kondo, H., Warren, G. FEBS Lett. (1998) [Pubmed]
  10. Glucose, palmitate and pro-inflammatory cytokines modulate production and activity of a phagocyte-like NADPH oxidase in rat pancreatic islets and a clonal beta cell line. Morgan, D., Oliveira-Emilio, H.R., Keane, D., Hirata, A.E., Santos da Rocha, M., Bordin, S., Curi, R., Newsholme, P., Carpinelli, A.R. Diabetologia (2007) [Pubmed]
  11. Binding sites for hepatocyte nuclear factor 3 beta or 3 gamma and pancreas transcription factor 1 are required for efficient expression of the gene encoding pancreatic alpha-amylase. Cockell, M., Stolarczyk, D., Frutiger, S., Hughes, G.J., Hagenbüchle, O., Wellauer, P.K. Mol. Cell. Biol. (1995) [Pubmed]
  12. H2O2 activates Nox4 through PLA2-dependent arachidonic acid production in adult cardiac fibroblasts. Colston, J.T., de la Rosa, S.D., Strader, J.R., Anderson, M.A., Freeman, G.L. FEBS Lett. (2005) [Pubmed]
WikiGenes - Universities