Disease relevance of ACS1

• Binding of Adr1p synthesized by Escherichia coli to the ACS1 control region was shown by an electrophoretic mobility shift assay [1].

High impact information on ACS1

• The assay is based upon the quantitation of propionic acid using acetyl coenzyme A synthetase isolated from baker's yeast [2].
• A link between transcription and intermediary metabolism: a role for Sir2 in the control of acetyl-coenzyme A synthetase [3].
• Enzyme activity measurements and mRNA analyses showed that ACS1 and ACS2 were both expressed during carbon-limited growth on glucose, ethanol, and acetate in aerobic chemostat cultures [4].
• Experiments in which ethanol was pulsed to aerobic ethanol-limited chemostat cultures indicated that, in addition to glucose, ethanol also repressed ACS1 transcription, although to a lesser extent [4].
• Acetyl coenzyme A synthetase (EC 6.2.1.1) has been examined for its ability to accept various carboxylic acids as substrates in place of acetic acid [5].

Biological context of ACS1

• Disruption of the recently cloned ACS1 gene [De Virgilio, C., Burckert, N., Barth, G., Neuhaus, J., Boller, T. & Wiemken, A. (1992) Yeast 8, 1043-1051] did not cause an apparent phenotype, except for a prolonged lag-phase during growth on glucose or C2 compounds such as acetate and ethanol [6].
• The combined influence of the regulated UAS elements, CSRE and Adr1p binding site, mediates a strong increase in ACS1 expression under derepressing conditions [1].
• Site-directed mutagenesis of Abf1p binding sites in the ACS1 promoter significantly reduced gene expression in the ume6 mutant, grown under repressing conditions [1].
• Inactivation kinetics of the ACS1 product resembled those of isocitrate lyase [7].
• The ACS1 gene, encoding acetyl-coenzyme A synthetase, was mapped genetically at the left arm of chromosome I between pURA3 and PYK1 at 19 and 28 cM respectively [8].

Anatomical context of ACS1

• No peroxisomal remnants were observed inside vacuoles in the cells of acs1, acs2, acs3 and icl1 mutants after prolonged cultivation in ethanol medium [9].

Associations of ACS1 with chemical compounds

• Batch and glucose-pulse experiments revealed that transcription of ACS1 is subject to glucose repression [4].
• The yeast Saccharomyces cerevisiae contains two acetyl-CoA synthetase genes, ACS1 and ACS2 [10].
• Adenosine 5'-tetraphosphate and adenosine 5'-pentaphosphate are synthesized by yeast acetyl coenzyme A synthetase [11].
• Three target genes for the transcriptional activator Cat8p of Kluyveromyces lactis: acetyl coenzyme A synthetase genes KlACS1 and KlACS2 and lactate permease gene KlJEN1 [12].
• It is shown that a high level of ACS activity is necessary for full expression of the glyoxylate cycle enzymes [13].

Other interactions of ACS1

• In a mutant lacking the URS1-binding factor, Ume6p, ACS1 expression was partially glucose insensitive [1].
• (iv) The sequence ACGTSCRGAATGA was found in the promoters of the partially ethanol-repressed genes ACS1 and YAT1 [14].
• Gene ICL2 was identified as the structural gene encoding the monomer of ACS [13].

Analytical, diagnostic and therapeutic context of ACS1

• Western blots demonstrated that the ACS1 product, but not the ACS2 product, was degraded after a glucose pulse [7].
• The sequence analysis shows that ACS3 is a pseudogene representing a truncated version of ACS1 [15].
• Studies on the "aerobic" acetyl-coenzyme A synthetase of Saccharomyces cerevisiae: purification, crystallization, and physical properties of the enzyme [16].

References