Disease relevance of ECs1712

• Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene [1].
• Phosphoribosylpyrophosphate synthetase of Bacillus subtilis. Cloning, characterization and chromosomal mapping of the prs gene [2].
• Structure of the gene encoding phosphoribosylpyrophosphate synthetase (prsA) in Salmonella typhimurium [3].
• Unlike virulence determinants associated with urinary tract infection and/or neonatal meningitis (pap or prs, sfa, kps, and hly), the 2.1-kb insertion is shared by all group B2 strains of the E. coli Reference Collection [4].
• The gene, prs, encoding phosphoribosylpyrophosphate (PRPP) synthetase of Escherichia coli was isolated from a library of E. coli genes cloned in the bacteriophage lambda D69 vector [5].

High impact information on ECs1712

• It has been previously proposed that a divalent cation binds to PRPP synthetase and serves as a bridge to the alpha-phosphate of ATP and AMP at the active site [6].
• Phosphoribosylpyrophosphate (P-Rib-PP) synthetase of Escherichia coli has been purified to near homogeneity from a strain harboring the prs gene, encoding P-Rib-PP synthetase, on a multicopy plasmid [1].
• The nucleotide sequence of the E. coli prs gene has been determined and the coding segment established [1].
• The deduced amino acid sequence of P-Rib-PP synthetase contained 314 amino acid residues and the molecular weight was calculated as 34,060 [1].
• Immediately downstream of the trp operon are genes encoding kynureninase and ribose phosphate pyrophosphokinase [7].

Chemical compound and disease context of ECs1712

• Complementation analyses, using various mutant Escherichia coli isolates, indicate that the tryptophan biosynthesis, kynureninase and ribose phosphate pyrophosphokinase gene products are functional [7].
• The prs gene encoding phosphoribosyl diphosphate (PRPP) synthase of the hyperthermophilic autotrophic methanogenic archaeon Methanocaldococcus jannaschii has been cloned and expressed in Escherichia coli [8].
• Mutation in the phosphoribosylpyrophosphate synthetase gene (prs) that results in simultaneous requirements for purine and pyrimidine nucleosides, nicotinamide nucleotide, histidine, and tryptophan in Escherichia coli [9].

Biological context of ECs1712

• Horizontal gene transfer of the Escherichia coli pap and prs pili operons as a mechanism for the development of tissue-specific adhesive properties [10].
• Initiation of replication of the Escherichia coli chromosome is rendered temperature-sensitive by the dnaR130 mutation in the prs gene that encodes phosphoribosylpyrophosphate synthetase [11].
• We now describe a novel gene cluster from J96, prs, which is responsible for the agglutination of sheep erythrocytes [12].
• A group of genes (cnf1, hra, hly, and prs) known to be present in a pathogenicity island of the uropathogenic strain E. coli J96 colocalized with region 6 [13].
• It is linked to the P-pilus (prs) and F1C fimbrial (foc) gene clusters on a pathogenicity island and appears to have been acquired by IS1230-mediated horizontal transmission [14].

Associations of ECs1712 with chemical compounds

• The DNA fragment was found to include the entire prs-coding sequence and specify a 34,000 M(r) protein with phosphoribosylpyrophosphate synthetase activity [15].
• Despite the lack of a detectable PRPP pool or PRPP synthetase activity at 40 degrees C, the strain was fully viable at this temperature as long as guanosine, uridine, histidine, tryptophan, and nicotinamide mononucleotide were all added to the growth medium [9].
• Of five genes examined in which the putative pur operator is upstream of the coding sequence, glnB, prsA, and speA bound purified purine repressor in vitro [16].
• In vivo, the usual function of PRPP synthetase is to synthesize PRPP from ribose-5-phosphate and ATP [17].
• The prs suppressor mutations led to the synthesis of a new phosphoryl compound that may act as a precursor for a new NAD biosynthetic pathway [18].

Analytical, diagnostic and therapeutic context of ECs1712

• By a complementation test using Kohara's mini-set of clones and by subcloning of a fragment from the phage clone 246, we found that both mutations were in the prs gene that encodes phosphoribosyl pyrophosphate synthetase [19].
• Northern blot analysis showed that the prs message in E. coli was on 1.3 and 2.7 kb transcripts [20].

References