Disease relevance of Microscopy, Polarization

• New advances in polarized light microscopy were used to image Congo red-stained cerebral amyloidosis in sharp relief [1].
• A study of isoproterenol-induced (1 mg/kg) myocardial fibrosis in the rat was undertaken, taking advantage of the differential colorization provided by thick and thin collagen fibers to picrosirius red and polarization microscopy [2].
• DESIGN--We evaluated 22 pheochromocytomas using routine hematoxylin-eosin and Congo red stains, polarization microscopy, and electron microscopy [3].
• Twelve segments of aortic arch containing atherosclerotic plaques obtained from cadavers were imaged with an 8-MHz US transducer, then sectioned, stained with picrosirius red and examined with polarized light microscopy [4].
• CONCLUSION: Polarization microscopy and sirius red staining is an ideal method in analyzing the distribution and arrangement of the collagen in hypertrophic scars [5].

High impact information on Microscopy, Polarization

• Arrays of prion rods ultrastructurally resembled purified amyloid and showed green birefringence by polarization microscopy after staining with Congo red dye [6].
• Here we utilize modulated polarization microscopy and computerized 3D reconstruction of tubulin and LFA-1 immunofluorescence images to investigate how this is accomplished [7].
• Polarized light microscopy of fresh, minced tissue showed rare droplets of free cholesterol ester [8].
• Some crystals were rod-like and had positive birefringence, thus tending to be confused with calcium pyrophosphate dihydrate when observed with only compensated polarized light microscopy [9].
• The mesomorphic phases of the cholesteryl oleate/probucol mixtures were identified by visual inspection and polarized light microscopy [10].

Biological context of Microscopy, Polarization

• The structural arrangement of collagen fibers in cervical ripening was studied in normal pregnant rats by picrosirius red staining and polarized light microscopy [11].
• Fibril formation of synthetic peptides having sequence homology with wild-type or mutant gelsolins was monitored by Congo-red staining and polarization microscopy, negative staining electron microscopy and quantitative thioflavine-T fluorometry [12].

Anatomical context of Microscopy, Polarization

• The orientation of an amphipathic, long acyl chain fluorescent carbocyanine dye [diI-C18-(3)] in a biological membrane is examined by steady-state fluorescence polarization microscopy on portions of single erythrocyte ghosts [13].
• Abdominal subcutaneous fat pad aspiration (ASFA) was performed, and smears were stained with Congo red and observed for apple-green birefringence under polarized light microscopy [14].
• OBJECTIVE: To study the fibrillar nature and structural features of the collagenous interstitium of the myocardium in normal and cardiomyopathic human hearts, employing the picrosirius red technique and polarization microscopy [15].
• A patient with pyrophosphate arthropathy is reported who had no calcifications on joint radiographs, and no crystals were found in polarized light microscopy of the synovial fluid [16].
• Congo red plus polarization microscopy failed to impart birefringence to the elastic fibers, but they could be identified by their intense staining and isotropic appearance against the bright background of birefringent collagen fibers [17].

Associations of Microscopy, Polarization with chemical compounds

• Particles were detected by polarized light microscopy and alizarin red staining [18].
• The morphologic appearance and volume fractions of interstitial (matrix) and perivascular (adventitial) collagen were analyzed with polarization microscopy and computerized videodensitometry in picrosirius red-stained sections [19].
• Polarized light microscopy, however, revealed the calcifications to be calcium oxalate crystals [20].
• However, postmortem polarization microscopy of all 24 canthaxanthin-treated animals showed a circular zone in the peripheral retina containing birefringent, polymorphous red, orange, or white inclusions [21].
• Polarized light microscopy at 37 degrees C revealed a multiplicity of brilliant white inclusions between crossed polars in cells incubated with palmitic acid [22].

Gene context of Microscopy, Polarization

• The thermotropic liquid crystalline behavior of these compounds was investigated by polarized light microscopy, DSC and X-ray scattering, and the influence of the length of the lateral chain on the mesomorphic properties was studied [23].
• Routine screening of periapical lesions with polarization microscopy and identification of possible cellulose fibers with Calcofluor White is advocated [24].
• To relate the present results to earlier studies, we examined mixtures of actin and filamin using a macroscopic assay of gelation (tube tipping assay), and polarized light microscopy [25].
• Following caries-like lesion formation (modified ten Cate solution), longitudinal sections (three per tooth quarter, 54 lesions per group) were obtained and evaluated for mean lesion depths (water imbibition, polarized light microscopy, ANOVA, DMR) [26].

Analytical, diagnostic and therapeutic context of Microscopy, Polarization

• Amyloid formation was monitored by Congo-red staining and polarization microscopy of the peptide aggregates, by negative staining electron microscopy, and by quantitative fluorometry with thioflavine T [27].
• The phase diagram for the binary system nafcillin sodium-water, was determined using differential scanning calorimetry (DSC) and polarized light microscopy [28].
• The lesions were evaluated by light microscopy (LM) (hematoxylin and eosin, Azan, van Gieson's, and Masson-Goldner's trichrome stains), transmission electron microscopy (TEM), and polarization microscopy after Sirius-red staining [29].
• Tissue from the dental periapical lesions of 36 rheumatoid and 23 control patients, as well as from the gingival buccal fold of 11 of the rheumatoid and 11 of the control patients, were examined by the direct immunofluorescence technique and by polarization microscopy of sections stained with Congo red [30].
• Vessels from autopsy were fixed at normal arterial distending pressure with 10% formalin, and prepared for polarized light microscopy, with paraffin embedding and staining with picrosirius red for birefringent enhancement [31].

References