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MeSH Review

Paraffin Embedding

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Disease relevance of Paraffin Embedding


High impact information on Paraffin Embedding

  • We demonstrated that the purified MoAb did not inhibit binding of MoAb CA19-9 to any colon Ca lines and reacted with fresh human colon carcinoma specimens regardless of whether they were processed by cryostat or paraffin embedding after fixation in formalin for 24 through 96 h [6].
  • The pathogenesis of 210Po-induced tumors in the peripheral lung of Syrian golden hamsters has been studied in a serial sacrifice experiment utilizing both plastic (glycol methacrylate) and routine paraffin embedding procedures for lung sections [7].
  • Here we describe an ethanol fixation and paraffin-embedding protocol that preserves tissue architecture and cellular morphology of the mouse endometrium, and allows for the recovery of high-quality RNA from microdissected cells [8].
  • However, when these tissues were studied after formalin fixation and paraffin embedding, a loss of nuclear staining was observed concurrent with the appearance of c-myc protein immunoreactivity in the cytoplasm [9].
  • AA-protein is not denatured by formalin and retains its typical electrophoretic, chromatographic, and immunologic characteristics even 30 years after fixation and paraffin-embedding [10].

Chemical compound and disease context of Paraffin Embedding


Biological context of Paraffin Embedding


Anatomical context of Paraffin Embedding


Associations of Paraffin Embedding with chemical compounds

  • This antibody recognizes a protein antigen at the cell surface, which is preserved after formalin fixation and paraffin embedding [20].
  • Using a general strategy for evaluating clinical tissue specimens, we found that 70% ethanol fixation and paraffin embedding is a useful method for molecular profiling studies [21].
  • The influence of the sequential stages of conventional formaldehyde fixation and paraffin embedding of cutaneous tissue on monoclonal antibody labeling of cell surface antigens is described [22].
  • Under light microscopy the storage material was granular, intensely PAS-positive and dissolved by paraffin embedding [23].
  • With increasing age, GFA protein could be demonstrated using higher aldehyde concentrations, longer fixation times, and longer paraffin embedding schedules [24].

Gene context of Paraffin Embedding


Analytical, diagnostic and therapeutic context of Paraffin Embedding


  1. A monoclonal antibody pool for routine immunohistochemical detection of human respiratory syncytial virus antigens in formalin-fixed, paraffin-embedded tissue. Wright, C., Oliver, K.C., Fenwick, F.I., Smith, N.M., Toms, G.L. J. Pathol. (1997) [Pubmed]
  2. The use of different fixatives and hydrophilic embedding media (Historesin and Unicryl) for the study of embryonic tissues. González Santander, R., Martínez Cuadrado, G., González-Santander Martínez, M., Monteagudo, M., Martínez Alonso, F.J., Toledo Lobo, M.V. Microsc. Res. Tech. (1997) [Pubmed]
  3. The relationship between cyclooxygenase-2 expression and characteristics of malignant transformation in human colorectal adenomas. Sheehan, K.M., O'Connell, F., O'Grady, A., Conroy, R.M., Leader, M.B., Byrne, M.F., Murray, F.E., Kay, E.W. European journal of gastroenterology & hepatology. (2004) [Pubmed]
  4. Comparison of snap freezing versus ethanol fixation for gene expression profiling of tissue specimens. Perlmutter, M.A., Best, C.J., Gillespie, J.W., Gathright, Y., González, S., Velasco, A., Linehan, W.M., Emmert-Buck, M.R., Chuaqui, R.F. The Journal of molecular diagnostics : JMD. (2004) [Pubmed]
  5. Comparison of static and flow cytofluorometry for estimation of DNA index and S-phase fraction in fresh and paraffin-embedded breast carcinoma tissue. Wingren, S., Hatschek, T., Stål, O., Boeryd, B., Nordenskjöld, B. Acta oncologica (Stockholm, Sweden) (1988) [Pubmed]
  6. New monoclonal antibodies against colon cancer-associated antigens. Drewinko, B., Yang, L.Y., Chan, J., Trujillo, J.M. Cancer Res. (1986) [Pubmed]
  7. Serial sacrifice study of pathogenesis of 210Po-induced lung tumors in Syrian golden hamsters. Kennedy, A.R., McGundy, R.B., Little, J.B. Cancer Res. (1978) [Pubmed]
  8. Expression profiling of mouse endometrial cancers microdissected from ethanol-fixed, paraffin-embedded tissues. Kabbarah, O., Pinto, K., Mutch, D.G., Goodfellow, P.J. Am. J. Pathol. (2003) [Pubmed]
  9. c-myc protein in normal tissue. Effects of fixation on its apparent subcellular distribution. Loke, S.L., Neckers, L.M., Schwab, G., Jaffe, E.S. Am. J. Pathol. (1988) [Pubmed]
  10. Demonstration of AA-protein in formalin-fixed, paraffin-embedded tissues. Shtrasburg, S., Pras, M., Langevitch, P., Gal, R. Am. J. Pathol. (1982) [Pubmed]
  11. A modification of the chronic tartrate silver impregnation technique for block impregnation of the central nervous system and paraffin wax embedding. Abadía-Fenoll, F., Ostos Garrido, M.V., López-Lendínez, J.L., Carmona Martos, R. Journal of microscopy. (1985) [Pubmed]
  12. Chemical dehydration for rapid paraffin embedding. Möller, W., Möller, G. Biotechnic & histochemistry : official publication of the Biological Stain Commission. (1994) [Pubmed]
  13. Immunohistochemical detection of steroid binding in rabbit cornea. Rojanasakul, Y., Robinson, J.R. Journal of ocular pharmacology. (1988) [Pubmed]
  14. Renal chromophobe cell carcinoma: limitations of paraffin-embedded tissue. Bonsib, S.M., Bray, C., Timmerman, T.G. Ultrastructural pathology. (1993) [Pubmed]
  15. Multifocal micronodular pneumocyte hyperplasia and lymphangioleiomyomatosis in tuberous sclerosis with a TSC2 gene. Maruyama, H., Seyama, K., Sobajima, J., Kitamura, K., Sobajima, T., Fukuda, T., Hamada, K., Tsutsumi, M., Hino, O., Konishi, Y. Mod. Pathol. (2001) [Pubmed]
  16. Detection of Epstein-Barr virus in CNS lymphomas by in-situ hybridization. Bashir, R.M., Harris, N.L., Hochberg, F.H., Singer, R.M. Neurology (1989) [Pubmed]
  17. Responses of the fetal cerebral vasculature to prenatal cytotoxic brain damage. Misztal, D.R., Ashwell, K.W., Zhang, L.L. Neurotoxicology and teratology. (1994) [Pubmed]
  18. Growth hormone regulates nucleolar organizer regions during odontogenesis in the rat. Zhang, C.Z., Young, W.G., Li, H., Rolinson, S., Waters, M.J. J. Oral Pathol. Med. (1992) [Pubmed]
  19. Experience with bone marrow trephine biopsy. Rege, J.D., Phatak, A.M., Issac, R., Bichile, S.K. The Journal of the Association of Physicians of India. (1992) [Pubmed]
  20. Immunohistochemical analysis of human pulmonary carcinomas using monoclonal antibody 44-3A6. Lee, I., Radosevich, J.A., Ma, Y.X., Combs, S.G., Rosen, S.T., Gould, V.E. Cancer Res. (1985) [Pubmed]
  21. Evaluation of non-formalin tissue fixation for molecular profiling studies. Gillespie, J.W., Best, C.J., Bichsel, V.E., Cole, K.A., Greenhut, S.F., Hewitt, S.M., Ahram, M., Gathright, Y.B., Merino, M.J., Strausberg, R.L., Epstein, J.I., Hamilton, S.R., Gannot, G., Baibakova, G.V., Calvert, V.S., Flaig, M.J., Chuaqui, R.F., Herring, J.C., Pfeifer, J., Petricoin, E.F., Linehan, W.M., Duray, P.H., Bova, G.S., Emmert-Buck, M.R. Am. J. Pathol. (2002) [Pubmed]
  22. Monoclonal antibody labeling of mononuclear cell surface antigens in formaldehyde-fixed paraffin-embedded cutaneous tissue. Cerio, R., Dupuy, P.F., Allen, M.H., MacDonald, D.M. J. Invest. Dermatol. (1986) [Pubmed]
  23. Chloroquine intoxication induces ganglioside storage in nervous tissue: a chemical and histopathological study of brain, spinal cord, dorsal root ganglia, and retinal in the miniature pig. Klinghardt, G.W., Fredman, P., Svennerholm, L. J. Neurochem. (1981) [Pubmed]
  24. Glial fibrillary acidic protein in the optic nerve of the developing albino rat: an immunoperoxidase study of paraffin-embedded tissue. Dixon, R.G., Eng, L.F. J. Comp. Neurol. (1981) [Pubmed]
  25. Immunohistochemical demonstration of nonspecific cross-reacting antigen in normal and neoplastic human tissues using a monoclonal antibody. Comparison with carcinoembryonic antigen localization. Tsutsumi, Y., Onoda, N., Misawa, M., Kuroki, M., Matsuoka, Y. Acta Pathol. Jpn. (1990) [Pubmed]
  26. Immunolocalization of acidic and basic fibroblast growth factors during mouse odontogenesis. Cam, Y., Neumann, M.R., Oliver, L., Raulais, D., Janet, T., Ruch, J.V. Int. J. Dev. Biol. (1992) [Pubmed]
  27. Expression of carcinoembryonic or related cross-reacting antigens in primary pediatric neuroectodermal brain tumors. Bodey, B., Siegel, S.E., Peacock, J.S. Mod. Pathol. (1992) [Pubmed]
  28. Nuclear p53 immunoreaction associated with poor prognosis of breast cancer. Iwaya, K., Tsuda, H., Hiraide, H., Tamaki, K., Tamakuma, S., Fukutomi, T., Mukai, K., Hirohashi, S. Jpn. J. Cancer Res. (1991) [Pubmed]
  29. Reaction patterns of monoclonal antibodies to HLA-G in human tissues and on cell lines: a comparative study. Blaschitz, A., Hutter, H., Leitner, V., Pilz, S., Wintersteiger, R., Dohr, G., Sedlmayr, P. Hum. Immunol. (2000) [Pubmed]
  30. Markers of cell proliferation in normal epithelia and dysplastic leukoplakias of the oral cavity. Liu, S.C., Sauter, E.R., Clapper, M.L., Feldman, R.S., Levin, L., Chen, S.Y., Yen, T.J., Ross, E., Engstrom, P.F., Klein-Szanto, A.J. Cancer Epidemiol. Biomarkers Prev. (1998) [Pubmed]
  31. Use of freeze-drying technology for the study of renal biopsies. Muda, A.O., Crescenzi, A., Mercorella, I., Faraggiana, T., Marinozzi, V. Mod. Pathol. (1990) [Pubmed]
  32. Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue. Biedermann, K., Dandachi, N., Trattner, M., Vogl, G., Doppelmayr, H., Moré, E., Staudach, A., Dietze, O., Hauser-Kronberger, C. J. Clin. Microbiol. (2004) [Pubmed]
  33. Colocalization of estrogen and progesterone receptors with an estrogen-regulated heat shock protein in paraffin sections of human breast and endometrial cancer tissue. Ciocca, D.R., Stati, A.O., Amprino de Castro, M.M. Breast Cancer Res. Treat. (1990) [Pubmed]
  34. Ultrasound-accelerated Tissue Fixation/Processing Achieves Superior Morphology and Macromolecule Integrity with Storage Stability. Chu, W.S., Liang, Q., Tang, Y., King, R., Wong, K., Gong, M., Wei, M., Liu, J., Feng, S.H., Lo, S.C., Andriko, J.A., Orr, M. J. Histochem. Cytochem. (2006) [Pubmed]
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