Disease relevance of ouabain

• These data indicate that blockade of brain "oubain" prevents sodium-induced hypertension as well as the desensitization of the arterial baroreflex in Dahl S rats [1].
• 1. The 60 mM K+, 152 mM K+, Na-deficient medium and oubain-induced contractions of aorta were not so affected by severe hypoxia [2].
• Quabain was injected intravenously until ventricular ectopic beats accounted for at least 60% of the heart rate [3].

High impact information on ouabain

• Increased brain "oubain" may desensitize the arterial baroreflex and thereby facilitate the hypertension in Dahl S rats on high sodium [1].
• The differential action of the cardiac glycosides oubain and stophanthidin on interneurons from control versus head-injured rats indicated that the depolarization of interneurons was related to the trauma-induced decrease in the activity of the electrogenic Na(+)/K(+)-ATPase [4].
• In preliminary experiments, point mutations as evidenced by oubain resistance were not observed [5].
• Interdependence of ion transport and the action of quabain in heart muscle [6].
• Oubain, a Na(+)/glucose symport inhibitor, did not inhibit glucose uptake [7].

Biological context of ouabain

• The effect of cyclopropane and N2O, oubain, and aldosterone on muscle contraction closely paralleled their action on electrogenic Na+ transport [8].
• Laidlomycin propionate decreased (P = 0.032) the percentage of whole jejunum oxygen consumption due to oubain-sensitive respiration compared with control [9].
• Cell growth and quabain-sensitive 86Rg+ uptake and (Na++K+)-ATPase activity in 3T3 and SV40 transformed 3T3 fibroblasts [10].
• In women, the diastolic blood pressure correlated negatively with total Zn efflux from lymphocytes (r = -0.39), oubain-dependent Zn efflux from lymphocytes (r = -0.49), and serum Zn (r = -0.46); systolic blood pressure correlated negatively with lymphocyte zinc (r = -0.38) [11].

Anatomical context of ouabain

• Experiments in which 24-Na, amiloride, or sodium-free mucosal solutions were used, revealed that a large, though variable, percentage of the sodium gained by cells exposed to oubain, came from the mucosal medium, a finding consistent with the model of passive sodium entry from the mucosal medium followed by active sodium extrusion to the serosa [12].
• 5. Phosphoprotein formation and ATP-ADP phosphate exchange are not affected by azide, dinitrophenol, dicyclohexyl carbodiimide and oubain, whilst both activities are reduced by blockade of -SH groups localized on the outside of the sarcoplasmic reticulum membrane [13].
• The ultrastructural cytochemical localization of a potassium-dependent oubain-sensitive nitrophenyl phosphatase (transport ATPase) activity in human blood platelets is described [14].
• A panel of three bispecific monoclonal antibodies (bsMAbs) binding to follitropin (FSH) and to beta-galactosidase have been prepared by fusion of hybridoma cell lines resistant to oubain and neomycin [15].
• Different sensitivities of acetylcholine-induced "after-hyperpolarization" compared to dopamine-induced hyperpolarization, to oubain or to lithium-replacement of sodium, in rabbit sympathetic ganglia [16].

Associations of ouabain with other chemical compounds

• HL-60 sub-lines selected by exposure to a single high concentration of 5-bromo-2'-deoxyuridine (BUdR; 3.3 X 10(-2)mM) or oubain (Ou; 5 X 10(-3)mM) were not or were slightly cross-resistant to either granulocyte or monocyte inducers [17].

Gene context of ouabain

• Oubain increased blood glucose level from 114.1 plus minus 3.8 (control) to 152.1 plus minus 5.3 mg x dl (-1) [18].

References