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Gene Review

Crybb2  -  crystallin, beta B2

Mus musculus

Synonyms: Aey2, Beta-B2 crystallin, Beta-crystallin B2, Beta-crystallin Bp, Cryb-2, ...
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Disease relevance of Crybb2

  • Using rat epsilon BP cDNA, we have succeeded in expressing recombinant epsilon BP in Escherichia coli [1].
  • Influenza virus A/Phil/82 (H3N2) grew to significantly higher titers in the lungs of diabetic than nondiabetic mice [2].
  • We chose to study the effects of loss of GH signaling on growth and aging in a mouse model for Laron Syndrome (LS) in which the GHR/BP gene has been disrupted [3].
  • In their study Philp and coworkers address the issue of the effect of 17beta-estradiol on ventricular vulnerability in a rat model of ischemia [4].
  • While mutations in betaB2-crystallin are known to result in lens opacities, alterations in tissues besides the lens have not been previously investigated in these mutants [5].

High impact information on Crybb2

  • To create a mammalian model for this disease, we generated mice bearing a disrupted GHR/binding protein (GHR/BP) gene through a homologous gene targeting approach [6].
  • The purified r epsilon BP exhibits binding activity to various saccharides, with affinity for N-acetyllactosamine greater than thiodigalactoside greater than lactose much greater than D-galactose greater than L-arabinose, an order identical to that exhibited by native epsilon BP isolated from RBL cells [1].
  • Although epsilon BP shares sequence homology to other lectins containing S-type (thiol-dependent) carbohydrate-recognition domains, r epsilon BP is resistant to air oxidation and does not require reducing agents for maintaining its activity [1].
  • The availability of a source for a large quantity of epsilon BP should facilitate the analysis of biological function(s) and structure-activity relationships of this lectin [1].
  • More recently, CBP35 (epsilon BP) was found to be equivalent to Mac-2, a surface marker on activated macrophages [1].

Biological context of Crybb2

  • Two of them are lens fiber cell structural proteins: the Cryba1 locus encoding crystallinbetaA3/A1 maps to chromosome 11, 2.5 +/- 2.5 cM distal to D11Mit31, and the Crybb2 locus encoding crystallinbetaB2 maps to chromosome 5, 9.1 +/- 4.3 cM distal to D5Mit88 [7].
  • Sequence, initial functional analysis and protein-DNA binding sites of the mouse beta B2-crystallin-encoding gene [8].
  • An 800-bp fragment of genomic DNA upstream from the origin of transcription of the mouse beta B2-crystallin-encoding gene (beta B2-Cry) has been isolated and its nucleotide sequence determined [8].
  • The epsilon BP gene is composed of six exons separated by five introns [9].
  • This alternative splicing results in different 5' untranslated regions of the murine epsilon BP mRNA [9].

Anatomical context of Crybb2

  • Surprisingly, blot hybridization analysis of RNA from nine various normal rat tissues showed that the epsilon BP gene is transcribed in all the tissues tested as well as in a mouse macrophage-like cell line [10].
  • We show that IgE-binding protein (epsilon BP) is found primarily in the cytoplasm of rat basophilic leukemia (RBL) cells and COS-1 cells transfected with epsilon BP cDNA [10].
  • RESULTS: In the mouse testis, betaB2-crystallin mRNA is found at low levels at birth, but its expression upregulates in this tissue as the testis is primed to initiate spermatogenesis [5].
  • To characterize alternative 5'UTR usage in mouse GHR/BP mRNA, we carried out 5' rapid amplification of cDNA ends using RNA from non-pregnant mouse liver and adipose tissue [11].
  • Western blotting detected betaB2-crystallin protein in sperm obtained from mice, cattle, and humans while immunolocalization detected this protein in developing sperm from the spermatocyte stage onward [5].

Associations of Crybb2 with chemical compounds

  • Milligram quantities of homogeneous epsilon BP could be obtained from bacterial lysate in a one-step affinity purification procedure utilizing lactosyl-Sepharose 4B and elution with a lactose gradient [1].
  • To elucidate the novel WNK1 BP control pathway active in distal nephron, WNK1 expression in mouse was studied [12].
  • Glucose is a ligand for SP-D, and neutralization of A/Phil/82 virus by SP-D was abolished in the presence of glucose at levels commonly found in diabetic mice [2].

Analytical, diagnostic and therapeutic context of Crybb2


  1. Expression of biologically active recombinant rat IgE-binding protein in Escherichia coli. Frigeri, L.G., Robertson, M.W., Liu, F.T. J. Biol. Chem. (1990) [Pubmed]
  2. Increased susceptibility of diabetic mice to influenza virus infection: compromise of collectin-mediated host defense of the lung by glucose? Reading, P.C., Allison, J., Crouch, E.C., Anders, E.M. J. Virol. (1998) [Pubmed]
  3. Assessment of growth parameters and life span of GHR/BP gene-disrupted mice. Coschigano, K.T., Clemmons, D., Bellush, L.L., Kopchick, J.J. Endocrinology (2000) [Pubmed]
  4. Sex hormones and arrhythmia in myocardial ischemia. Korte, T., Grohé, C. Br. J. Pharmacol. (2006) [Pubmed]
  5. Subfertility in mice harboring a mutation in betaB2-crystallin. Duprey, K.M., Robinson, K.M., Wang, Y., Taube, J.R., Duncan, M.K. Mol. Vis. (2007) [Pubmed]
  6. A mammalian model for Laron syndrome produced by targeted disruption of the mouse growth hormone receptor/binding protein gene (the Laron mouse). Zhou, Y., Xu, B.C., Maheshwari, H.G., He, L., Reed, M., Lozykowski, M., Okada, S., Cataldo, L., Coschigamo, K., Wagner, T.E., Baumann, G., Kopchick, J.J. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  7. Mapping of four mouse genes encoding eye lens-specific structural, gap junction, and integral membrane proteins: Cryba1 (crystallin beta A3/A1), Crybb2 (crystallin beta B2), Gja8 (MP70), and Lim2 (MP19). Kerscher, S., Church, R.L., Boyd, Y., Lyon, M.F. Genomics (1995) [Pubmed]
  8. Sequence, initial functional analysis and protein-DNA binding sites of the mouse beta B2-crystallin-encoding gene. Chambers, C., Cvekl, A., Sax, C.M., Russell, P. Gene (1995) [Pubmed]
  9. Genomic cloning of the gene for an IgE-binding lectin reveals unusual utilization of 5' untranslated regions. Gritzmacher, C.A., Mehl, V.S., Liu, F.T. Biochemistry (1992) [Pubmed]
  10. IgE-binding protein. Subcellular location and gene expression in many murine tissues and cells. Gritzmacher, C.A., Robertson, M.W., Liu, F.T. J. Immunol. (1988) [Pubmed]
  11. Alternative 5'-untranslated regions of mouse GH receptor/binding protein messenger RNA are derived from sequences adjacent to the major L2 promoter. Moffat, J.G., Dao, H., Talamantes, F. J. Endocrinol. (2000) [Pubmed]
  12. WNK1, a gene within a novel blood pressure control pathway, tissue-specifically generates radically different isoforms with and without a kinase domain. O'Reilly, M., Marshall, E., Speirs, H.J., Brown, R.W. J. Am. Soc. Nephrol. (2003) [Pubmed]
  13. Aey2, a new mutation in the betaB2-crystallin-encoding gene of the mouse. Graw, J., Löster, J., Soewarto, D., Fuchs, H., Reis, A., Wolf, E., Balling, R., Hrabé de Angelis, M. Invest. Ophthalmol. Vis. Sci. (2001) [Pubmed]
  14. Deletion mutation in an eye lens beta-crystallin. An animal model for inherited cataracts. Chambers, C., Russell, P. J. Biol. Chem. (1991) [Pubmed]
  15. Energetics of domain-domain interactions and entropy driven association of beta-crystallins. Sergeev, Y.V., Hejtmancik, J.F., Wingfield, P.T. Biochemistry (2004) [Pubmed]
  16. Association properties of betaB2- and betaA3-crystallin: ability to form dimers. Hejtmancik, J.F., Wingfield, P.T., Chambers, C., Russell, P., Chen, H.C., Sergeev, Y.V., Hope, J.N. Protein Eng. (1997) [Pubmed]
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