Disease relevance of Cd207

• CD8 alpha- and Langerin-negative dendritic cells, but not Langerhans cells, act as principal antigen-presenting cells in leishmaniasis [1].

High impact information on Cd207

• Langerin is a C-type lectin expressed by a subset of dendritic leukocytes, the Langerhans cells (LC) [2].
• Functionally, the langerin(-/-) LC were able to capture antigen, to migrate towards skin draining lymph nodes, and to undergo phenotypic maturation [2].
• Finally, chemical mutagenesis resulted in a similar rate of skin tumor development in langerin(-/-) and wild-type mice [2].
• Langerin is a cell surface receptor that induces the formation of an LC-specific organelle, the Birbeck granule (BG) [2].
• Notably, the Birbeck granule-associated Langerin antigen, as well as CD103 (the mouse homologue of the rat DC marker OX62), were specifically expressed by the CD8alpha+ DC subset [3].

Biological context of Cd207

• The organization of human and mouse Langerin genes are similar, consisting of six exons, three of which encode the carbohydrate recognition domain [4].
• The cognate mouse and rat genes were assigned to chromosome 6D1-D2 and chromosome 4q33 distal-q34.1 proximal respectively, syntenic to the h-Langerin gene on chromosome 2p13 [5].
• Characterization of antigen-positive cells showed that L. major co-localized with DC of a CD11c(+) CD8 alpha(-) Langerin(-) phenotype [1].
• Nevertheless, these LC expressed normal levels of major histocompatibility complex (MHC)-class II and Langerin/ CD207 in situ [6].
• The use of LC-specific markers like Langerin or knock-in mice expressing green fluorescent protein under the control of the Langerin promotor now facilitates the dissection of LCs from other dendritic cell (DC) subsets [7].

Anatomical context of Cd207

• Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues [4].
• Fibroblasts transfected with mouse Langerin cDNA contained typical Birbeck granules (BG) and cored tubules, whereas DeltaE3Langerin cDNA did not induce BG or cored tubule formation in transfected fibroblasts [8].
• DC derived from blood monocytes by GM-CSF + IL-4 lacked m-Langerin unless the cultures were supplemented with transforming growth factor (TGF)-beta1 [5].
• DC generated from bone marrow precursors by granulocyte macrophage colony stimulating factor (GM-CSF) expressed m-Langerin, but this was shut down during maturation with CD40 ligand or lipopolysaccharide [5].
• Without addition of exogenous antigen only the CD8 alpha(-) Langerin(-) DC were capable of stimulating antigen-specific T cell proliferation [1].

Associations of Cd207 with chemical compounds

• Most notably, the estrogen-dependent CD11b(int)Ly6C(-) DC express langerin (CD207) and contain Birbeck granules characteristic of Langerhans cells [9].
• A major lung CD103 (alphaE)-beta7 integrin-positive epithelial dendritic cell population expressing Langerin and tight junction proteins [10].
• Langerin/CD207 is a mannose-binding C-type lectin that is specifically expressed by LC [11].
• Increased numbers of Langerhans cells, as determined by immunostaining for Langerin/CD207, appeared in the lymph nodes in response to a contact allergen [12].
• However, Langerin did bind to surfaces of primary skin fibroblasts and NIH3T3 cells. "Ligand blotting" of fibroblast membrane-enriched fractions with Langerin revealed reproducible binding to 140 and 240 kDa proteins resolved in reduced denaturing gels [13].

Other interactions of Cd207

• Mouse Langerin protein isoforms were readily detected in fibroblasts transfected with cDNAs encoding epitope-tagged Langerin and DeltaE3Langerin [8].
• High and low affinity carbohydrate ligands revealed for murine SIGN-R1 by carbohydrate array and cell binding approaches, and differing specificities for SIGN-R3 and langerin [14].

Analytical, diagnostic and therapeutic context of Cd207

• An alternatively spliced variant of mouse Langerin that lacked the extracellular neck domain (DeltaE3Langerin) was detected in RNA derived from FSDDC and epidermal LC by RT-PCR [8].
• Recombinant soluble Langerin did not bind to lymph node or spleen cells, or keratinocytes as assessed via flow cytometry [13].
• We have recently combined high resolution two-photon immunofluorescence (using anti MHC-II and Langerin/CD207 antibodies) imaging with microspectroscopy and advanced image-processing/volume-rendering modalities [15].

References