Disease relevance of LAMP3

• In the present study, HIV-1 p55Gag Ag was synthesized as a DNA plasmid with either lysosomal-associated membrane protein-1 (LAMP/gag) or human dendritic cell-LAMP (DC-LAMP/gag) and used to immunize mice [1].
• DC-LAMP staining was lost in solid type adenocarcinomas but persisted in well-differentiated areas [2].

High impact information on LAMP3

• We have identified a novel lysosome-associated membrane glycoprotein localized on chromosome 3q26.3-q27, DC-LAMP, which is homologous to CD68 [3].
• This suggests that DC-LAMP might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of DC [3].
• Mature DCs were defined by expression of CD83 and DC-Lamp [4].
• Since they also expressed DC-LAMP, these mDCs appear to correspond to interdigitating dendritic cells (IDCs) [5].
• During AIDS, DC-LAMP mRNA expression levels were significantly reduced in lymph nodes and spleen, and DC-SIGN levels were significantly reduced in spleen [6].

Biological context of LAMP3

• These findings indicated that up-regulation of the TSC403 transcript may be related to the development and/or progression of cancer in humans [7].
• The cDNA, designated TSC403, contained an open reading frame of 1248 nucleotides encoding 416 amino acids; the deduced amino acid sequence showed significant similarities to lysosomal membrane glycoproteins (lamps) 1 and 2 [7].
• However, LAMP/gag and DC-LAMP/gag plasmids also elicited significant responses to 23 additional cryptic epitopes that were not recognized after immunization with native gag DNA [1].
• DC-LAMP stains pulmonary adenocarcinoma with bronchiolar Clara cell differentiation [2].
• The vast majority of all types of dendritic cells in normal human skin was of immature phenotype, i.e. did not express DC-LAMP/CD208 [8].

Anatomical context of LAMP3

• We detected a high level of the TSC403 transcript in primary cancers of the esophagus, colon, fallopian tube, ovary, breast, and liver, although expression of this gene was barely detectable in corresponding normal tissues [7].
• Individually, DC-LAMP/gag elicited the most diverse response, with a specific T cell response against 35 peptides [1].
• When cells are treated with U18666A, a drug that mimics the Niemann-Pick type C syndrome, both proteins accumulate in late endosomes and fail to reach Weibel-Palade bodies efficiently, suggesting that P-selectin, like CD63/lamp3, cycles via late endosomes [9].
• Patients with relatively high numbers of mature CD208-positive infiltrating dendritic cells in the tumor epithelium had a shorter overall survival (P = 0.004) [10].
• Induction of lysosomal-associated membrane protein 3 required both hormones, and expression was localized to limiting membranes of lamellar bodies [11].

Associations of LAMP3 with chemical compounds

• Aerobic Oxidation of Benzyl- and Allylic Alcohols under Visible Light Irradiation of a Fluorescent Lamp in the Presence of Catalytic Iodine [12].

Other interactions of LAMP3

• Immature and mature DCs were defined by the expression of CD1a and DC-LAMP/CD83, respectively [13].
• It is striking that the LC subset is able to express a high level of CCR7 expression and the maturation marker DC-lysosome-associated membrane protein (DC-LAMP) [14].
• In addition, neutralization of IL-10 during the differentiation of DCs increased the expression of DC-LAMP and MHC class II by IFN-DCs, and the ability of IFN-DCs to stimulate allogeneic CD4(+) T-cell proliferation at similar levels, than IL-4-DCs [15].
• DC-LAMP and CC-10 reactivity was also observed in endometrial adenocarcinomas but not in other tumor types [2].
• Sequential colocalization of an asialoglycoprotein-pulse with rab7 and lamp3 demonstrates that early endosomes change into or fuse with late endosomes and lysosomes [16].

Analytical, diagnostic and therapeutic context of LAMP3

• Dendritic cells were identified with four different markers (S-100, HLA class II, CD208, and CD1a) in double immunohistochemistry, with laminin as second marker to support the exact localization [10].
• DC-LAMP marks pulmonary adenocarcinomas that show Clara cell characteristics by electron microscopy [2].
• In addition, 3 DC maturation markers not present on the microarrays (DEC205, DC LAMP and CCR7) were analyzed using real-time RT-PCR and found to be up-regulated at several time points [17].
• Confocal microscopy revealed abnormal distribution of LAMP-3 (lysosomal associated membrane protein-3) in fibroblasts from the patients, indicating abnormal trafficking of lysosomal lineage organelles [18].

References