Disease relevance of 10S

• Later, a sharp fall in "10S" and "4S" AChE occurs about seven days after denervation when muscle atrophy develops with loss of weight and proteins [1].
• (greater than 10S) virus RNA was inhibited in a manner parallel to that of total virus RNA, the synthesis of small (3S to 7S) virus RNA was inhibited by only 40 to 50% in the presence of DRB [2].

High impact information on 10S

• Nucleic acid hybridization analysis with a cDNA probe specific for the constant region of kappa light chains revealed that microsomal RNA from the wild-type cell contains both 14S and a 10S species of kappa specific RNA, whereas the variant contains only the 10S species [3].
• To assess the effect of CNLAC1 deletion on virulence, a Mel- mutant (10S) was obtained by disruption of the 5' end of the gene [4].
• Poly(A)-containing 10S human beta-globin mRNA was detected in the cytoplasm of the hybrid cells [5].
• Characterization of a Mr = 56,000 polypeptide associated with 10S DNA polymerase alpha purified from calf thymus using monoclonal antibody [6].
• As judges by sedimentation in SDS and in formamide gradients, the size of the RNA synthesized is heterogeneous from smaller than 10S to larger than 45S, thus resembling in vivo made RNA [7].

Biological context of 10S

• Reduced axonal transport of 10S acetylcholinesterase in dystrophic mice [8].
• The number of binding sites in preparations from Tfm carriers was intermediate between that in the Tfm and the normal female . In 0.5M KCl the 8S receptor of normal submandibular gland sediments at about 3S, and the 10S receptor of normal kidney sediments at about 4.5S [9].

Anatomical context of 10S

• However, as measured by the 24-hour accumulation of enzyme activity proximal to a ligature on the sciatic nerve, the axonal transport of 10S AChE was only about one third as great in dystrophic as in normal nerve [8].
• Production and characterization of monoclonal antibody against 10S DNA polymerase alpha from calf thymus [10].
• Ad37-injected corneas expressed adenoviral E1A 10S and E1B 19k mRNA but not IIIa, and viral titers had fallen two logs by day 4 after injection [11].
• The poly(A) + RNA was fractionated by sucrose density-gradient centrifugation and the 8S and 10S fractions were found to induce Ep(FMLC) synthesis when they were injected into the oocytes [12].
• The principal form of AChE in septal cells is the 10S isoform bound on the external plasma membrane [13].

Associations of 10S with chemical compounds

• Three forms of AChE, distinguished by their sedimentation on sucrose gradients, were synthesized: 4-6S, 10S, and 16S [14].
• Furthermore, pulse-chase experiments indicated that some large RNAs with globin mRNA sequences could be chased to 10S RNA during a 30 min chase in the presence of a high concentration of actinomycin D (10 microgram/ml) [15].
• 3-Phenyloctahydropyrido[2,1-c][1,4]oxazine hydrochloride and the 10R and 10S diastereomers have been synthesized from (+/-)-, (+)-, and (-)-2-piperidinemethanol [16].
• This complex was precipitated at 35 and 40% saturation with ammonium sulfate in the kidney and the submandibular gland, respectively, and had an approximate sedimentation coefficient of 8S in the submandibular gland and 10S in the kidney [9].
• To gain insight into the mechanisms of oncogenic transformation, we point mutated the first splice donor in the Ad12-E1A gene, leading to the loss of the Ad12-E1A(9.5S) and Ad12-E1A(11S/10S) proteins and to a conservative amino acid (aa) exchange at position aa 30 (valine vs. leucine) in the Ad12-E1A(13S) and Ad12-E1A(12S ) proteins [17].

Physical interactions of 10S

• The antigen complex is trypsin-sensitive collagenase insensitive and bands as a complex of 10S on sucrose gradients in NP40 [18].

Regulatory relationships of 10S

• The time course of recovery of the molecular forms in the diaphragm occurred rather quickly with the smaller 4S and 10S forms recovering to control levels faster than the larger 16S form [19].

Other interactions of 10S

• The 4-6S and 10S forms appeared 1 day after the cells began to fuse, whereas the 16S form appeared only 2 days after fusion began [14].
• 5080 T did not oligomerize beyond 5 to 10S in size compared with normal T, which oligomerized predominantly to 14 to 20S species [20].
• Decreased G4 (10S) acetylcholinesterase content in motor nerves to fast muscles of dystrophic 129/ReJ mice: lack of a specific compartment of nerve acetylcholinesterase [21]?
• Three size classes of virus specific poly-A containing mRNA are identified and they are 35S, 20S-25S, and 10S-15S [22].
• Analysis of the size-distribution of partially purified leucyl-tRNA synthetase complexes indicated that, while enzyme preparations from young animals tended to consist of complexes of larger sizes, those from older animals contained smaller complexes, a 10S complex being a major component [23].

Analytical, diagnostic and therapeutic context of 10S

• Septal cultures and septal-hippocampal cocultures contain principally (63% and 51%, respectively) the 10S isoform; whereas hippocampal cultures contain little (22%) of this AChE form [13].
• When cells labeled at this temperature are lysed on alkaline sucrose gradients and their DNA is sedimented by ultracentrifugation, the incorporation into DNA smaller than 10S (Okazaki pieces), is found to be normal or slightly inhibited [24].
• Anti-H-2d serum precipitated cell-free translation products of 17S (heavy chain) and 10S (beta 2-microglobulin) mRNA fractions obtained by centrifugation on 10-30% linear sucrose gradient. beta 2-Microglobulin synthesis was 5-fold higher as compared with the heavy chain synthesis [25].

References