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Gene Review

CNP  -  2',3'-cyclic nucleotide 3' phosphodiesterase

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Disease relevance of CNP


High impact information on CNP


Biological context of CNP


Anatomical context of CNP

  • We describe the isolation of cDNA clones for bovine brain 2',3'-cyclic-nucleotide 3'-phosphohydrolase (CNPase, EC, the third most abundant protein in central nervous system myelin [3].
  • The CNP activity was always higher in oligodendrocytes than in neurons, but not appreciably different from that in astrocytes from 20 days of age onward [4].
  • Molecular and biochemical characterization of a CNP-sensitive guanylyl cyclase in bovine tracheal smooth muscle [5].
  • Stimulation of basal plasma membrane GC activity by CNP was higher than that by ANP, whereas guanylin showed no effect, indicating that CNP-sensitive guanylyl cyclase (GC-B) is the predominant functional BTSM mGC subtype [5].
  • We describe here the immunohistochemical localization of CNP in adult bovine cerebrum and cerebellum [6].

Associations of CNP with chemical compounds

  • Additionally, CNP was able to reverse the chloride inhibition of BTSM mGC activity, suggesting that this is a novel G protein-coupled GC-B receptor [5].
  • 0. On SDS polyacrylamide gels, rat CNP appears as two protein bands of approximately 48,000 and 50,000 M.W., with an upper band intensity of about 1/10 that of the lower band [7].
  • From 15 g of rat brain, approximately 400 micrograms of pure CNP was obtained, with a specific activity of 1,200 (2',3'-cyclic AMP) units/mg protein [7].
  • Strong adenosine triphosphate inhibition of CNP-stimulated mGC activity supports the finding that the tracheal mGC isoform belongs to the natriuretic peptide-sensitive mGCs [5].
  • The muscarinic-agonist, muscarine, failed to increase cytosolic calcium, and was the weakest stimulator of cGMP production. cGMP formation, induced by sodium nitroprusside (SNP, 100 microM) and by C-type natriuretic peptide (CNP, 100 nM), was inhibited by 30-40% by increasing [Ca2+]i with ionomycin [8].

Other interactions of CNP

  • AEF derived from subcortical white matter contained two-fold more myelin basic protein and a three-fold greater content of 2' 3' cyclic nucleotide 3' phosphodiesterase (CNP) compared with AEF derived from well myelinated white matter [9].
  • The proteolipid protein has been purified in deoxycholate solutions by chromatography on a blue dye-ligand column, which retained all of the basic protein and 2',3'-cyclic nucleotide-3'-phosphodiesterase, and then on Sephacryl S300, which separated proteolipid protein from phospholipid and high-molecular-weight proteins [10].
  • Relative to other microsomal fractions, this same fraction was less enriched in a microsomal marker (cytochrome c reductase) and only moderately enriched in the activity of a myelin membrane marker (2',3' cyclic nucleotide 3' phosphohydrolase, CNPase) [11].
  • Axolemma-enriched fractions isolated from uniformly well-myelinated fibers (bovine spinal accessory nerve) contained lower CNPase activity and higher acetylcholinesterase activity than comparable fractions isolated from variably myelinated fibers (rabbit sciatic nerve and bovine intradural roots) [12].

Analytical, diagnostic and therapeutic context of CNP

  • We report here the specific and intense immunofluorescence staining of cultured oligodendrocytes obtained from the brains of mouse, rat, calf and human by rabbit antiserum specific for purified bovine CNP [13].
  • For this purpose, we treated the cell cultures with ANP, BNP and CNP, and modulation of PAI-1 synthesis was evaluated [14].
  • Much of the CNPase activity remained with the membrane fraction even after treatment with high concentrations of trypsin when WP were completely hydrolysed and no protein bands with M.W > 14KDa were detected on the gels [15].


  1. An immunochemical investigation of 2':3'-cyclic nucleotide 3'-phosphodiesterase (CNP) in bovine cerebrum and human oligodendroglioma. Sheedlo, H.J., Yaghmai, F., Wolfe, L., Sprinkle, T.J. J. Neurosci. Res. (1985) [Pubmed]
  2. cDNA cloning and amino acid sequence of bovine brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase. Kurihara, T., Fowler, A.V., Takahashi, Y. J. Biol. Chem. (1987) [Pubmed]
  3. Molecular cloning of the myelin specific enzyme 2',3'-cyclic-nucleotide 3'-phosphohydrolase. Vogel, U.S., Thompson, R.J. FEBS Lett. (1987) [Pubmed]
  4. Carbonic anhydrase, 5'-nucleotidase, and 2',3'-cyclic nucleotide-3'-phosphodiesterase activities in oligodendrocytes, astrocytes, and neurons isolated from the brains of developing rats. Snyder, D.S., Zimmerman, T.R., Farooq, M., Norton, W.T., Cammer, W. J. Neurochem. (1983) [Pubmed]
  5. Molecular and biochemical characterization of a CNP-sensitive guanylyl cyclase in bovine tracheal smooth muscle. Borges, A., de Villarroel, S.S., Winand, N.J., de Bécemberg, I.L., Alfonzo, M.J., de Alfonzo, R.G. Am. J. Respir. Cell Mol. Biol. (2001) [Pubmed]
  6. Immunohistochemical localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase in adult bovine cerebrum and cerebellum. Nishizawa, Y., Kurihara, T., Masuda, T., Takahashi, Y. Neurochem. Res. (1985) [Pubmed]
  7. Purification of rat 2',3'-cyclic nucleotide 3'-phosphodiesterase. Wells, M.R., Sprinkle, T.J. J. Neurochem. (1981) [Pubmed]
  8. Activation of NO:cGMP pathway by acetylcholine in bovine chromaffin cells. Possible role of Ca2+ in the down-regulation of cGMP signaling. Rodriguez-Pascual, F., Miras-Portugal, M.T., Torres, M. Biochem. Pharmacol. (1995) [Pubmed]
  9. Isolation and characterization of axolemma-enriched fractions from discrete areas of bovine CNS. Detskey, P.Z., Bigbee, J.W., DeVries, G.H. Neurochem. Res. (1988) [Pubmed]
  10. Structure of the proteolipid protein extracted from bovine central nervous system myelin with nondenaturing detergents. Smith, R., Cook, J., Dickens, P.A. J. Neurochem. (1984) [Pubmed]
  11. Isolation and characterization of unmyelinated axolemma from bovine splenic nerve. DeVries, G.H., Campbell, B., Saunders, R. J. Neurosci. Res. (1999) [Pubmed]
  12. Isolation and characterization of axolemma-enriched fractions from rabbit and bovine peripheral nerve. Yoshino, J.E., DeVries, G.H. Neurochem. Res. (1993) [Pubmed]
  13. Immunofluorescence demonstration of 2':3'-cyclic-nucleotide 3'-phosphodiesterase in cultured oligodendrocytes of mouse, rat, calf and human. Kim, S.U., McMorris, F.A., Sprinkle, T.J. Brain Res. (1984) [Pubmed]
  14. Natriuretic peptides reduce plasminogen activator inhibitor-1 expression in human endothelial cells. Pawłowska, Z., Jerczyńska, H., Szemraj, J., Barańska, P., Swiatkowska, M., Cierniewski, C.S. Cell. Mol. Biol. Lett. (2002) [Pubmed]
  15. Topology of Wolfgram proteins and 2', 3'-cyclic nucleotide 3'-phosphodiesterase in CNS myelin: studies with proteases. Vemuri, G.S., Rao, K.V., Sastry, P.S. Neurochem. Res. (1994) [Pubmed]
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