Disease relevance of HIVEP2

• Furthermore, like HIV-EP1, expression of HIV-EP2 mRNA was greatly induced by mitogen and phorbol ester treatment of Jurkat T cells, suggesting that HIV-EP2 acts in HIV production from latently infected T cells [1].
• In the present study, we investigated HIVEP2 gene abnormalities in microdissected breast cancer tissue [2].
• We now show that the EP element, a key regulatory sequence within hepatitis B virus enhancer I, also associates with MIBP1 and RFX1 [3].

High impact information on HIVEP2

• Of the several hundred sequences identified in this manner we focused on HIV-EP2/Schnurri-2, a member of a family of genes encoding related zinc finger transcription factors [4].
• Our data indicate that MBP-2 is a transcription factor involved in the regulation of MHC class I gene expression [5].
• The same antiserum also precipitates a protein of greater than 250 kDa from COS cells transfected with a MBP-2 expression vector [5].
• MHC-binding protein 2 (MBP-2) is a 275-kDa protein, containing two sets of widely separated zinc fingers and a stretch of highly acidic amino acids, a putative transactivation domain [5].
• One, named MBP-2 (major histocompatibility complex-binding protein 2), encodes a factor that has DNA-binding properties similar, if not identical, to the H2TF1 transcription factor [6].

Biological context of HIVEP2

• Sequence homologous to HIVEP3 exon 6 is not found in mouse nor are the paralogous genes HIVEP1 and HIVEP2 [7].
• Chromosome 11 contains the genes for human MBP1 (hMBP1) and a second novel MBP, hMBP2, in the centromere to 11q12 region [8].
• Overall, while conservation of hMBP2's amino acid sequence (63% identity with hMBP1) suggests a common function(s) with hMBP1, hMBP2's substantially reduced charge and the existence of the similar mMBP2 argue for additional, unique functions for hMBP2 [8].
• Frequent down-regulation of HIVEP2 in human breast cancer [2].
• The human gene encoding the human immunodeficiency virus type 1 enhancer binding protein HIV-EP2 has been isolated [9].

Anatomical context of HIVEP2

• Northern blot analysis of HIV-EP2 mRNA indicated relatively high expression in the T cell line Molt-4 and in some tumor cell lines [1].
• Like hMBP1, hMBP2 is in the eosinophil secondary granule [8].
• There is abundant mRNA for hMBP1 in both bone marrow (eosinophils and basophils) and placenta, but hMBP2 mRNA is present only in bone marrow (eosinophils) [8].
• Using Southern analysis of human-rodent somatic cell hybrid DNA with a human HIV-EP2-specific cDNA probe, the HIV-EP2 gene was assigned to chromosome 6 [9].
• In addition, we show that treatment with retinoic acid induces both MIBP1 and RFX1 protein, as well as their DNA-binding activity, upon granulocytic differentiation of HL60 cells, with a gel mobility pattern identical to that of HeLa cells [10].

Associations of HIVEP2 with chemical compounds

• MBP 2 appears to recognize the core sugars of asparagine-linked oligosaccharides as well as the terminal sugars [11].
• The other mannose-binding protein (MBP 2) has a native Mr of 200,000 with subunits of Mr 28,000 and is specific only for mannose and fucose [11].
• Our data confirm that metallothionein-like cadmium binding protein, MBP-1, has the same affinity for Zn, while MBP-2 displays comparatively higher affinity for Zn than Cd [12].
• Induction of Myc-intron-binding polypeptides MIBP1 and RFX1 during retinoic acid-mediated differentiation of haemopoietic cells [10].

Physical interactions of HIVEP2

• Screening by the yeast two-hybrid system revealed that the MIBP1 protein interacts with the Ski-interacting protein (SKIP) [13].

Other interactions of HIVEP2

• The DNA-binding property of HIV-EP2 was similar to that of HIV-EP1 [1].
• We previously demonstrated that MIBP1 and RFX1 polypeptides associate in vivo to form a complex that binds to the MIF-1 element in the c-myc gene and the major histocompatibility complex class II X-box recognition sequence [3].

Analytical, diagnostic and therapeutic context of HIVEP2

• We then investigated SSTR-2, SEF-2, and MIBP1 mRNA expression patterns in the developing and adult murine brain by Northern blotting and in situ hybridization [14].

References