Disease relevance of HIVEP3

• Here we report the cloning and characterization of HIVEP3, the newest member in the human immunodeficiency virus type 1 enhancer-binding protein family that encodes large zinc finger proteins and regulates transcription via the kappaB enhancer motif [1].
• Both RASSF1C and RASSF1A suppressed the tumorigenicity of the KRC/Y RCC cell line in SCID mice [2].
• DNA analysis showed that the teratoma was derived from KRC(-/-) embryonic stem cells [3].
• On the other hand, 13 of 58 RCC without metastases expressed alpha 4 chain, alpha 4 and beta 1 expressions were also detected on 5 out of 5 human RCC cell lines, ACHN, KRC/Y, A498, Caki1 and Caki2, by flow-cytometric analysis [4].
• Establishment and characterization of a new human renal cell carcinoma cell line (KRC/Y) [5].

High impact information on HIVEP3

• The second, named KBP-1 (kappa-enhancer binding protein 1), encodes a factor that is similar to, but distinct from, NF-kappa B in its DNA-binding properties and methylation interference pattern, suggesting that at least two proteins can bind to the immunoglobulin kappa gene enhancer [6].
• KRC encodes a large zinc finger protein that binds to the kappaB motif and to the signal sequences of V(D)J recombination [7].
• In addition, RASSF1C was shown to induce cell cycle arrest in KRC/Y cells [2].
• The large zinc finger protein KRC regulates transcription of target genes via the kappaB gene enhancer element [8].
• Downregulation of KRC induces proliferation, anchorage independence, and mitotic cell death in HeLa cells [8].

Biological context of HIVEP3

• Structure of the human zinc finger protein HIVEP3: molecular cloning, expression, exon-intron structure, and comparison with paralogous genes HIVEP1 and HIVEP2 [1].
• The HIVEP3 gene is located in the chromosomal region 1p34 and is at least 300 kb with 10 exons [1].
• The largest open reading frame of HIVEP3 contains 2406 aa. and is approximately 80% identical to the mouse counterpart [1].
• Therefore, HIVEP3 may produce protein isoforms that contain or exclude the carboxyl DNA binding domain and the leucine zipper by alternative RNA splicing and differential polyadenylation [1].
• Sequence homologous to HIVEP3 exon 6 is not found in mouse nor are the paralogous genes HIVEP1 and HIVEP2 [1].

Anatomical context of HIVEP3

• Human renal cell cancer (RCC) cell lines, ACHN and KRC/Y, with or without exposure to cytokines, were examined for their susceptibility to lymphokine-activated killer (LAK) cells [9].
• As predicted from cDNAs, 5' untranslated region composed of the 2317 nucleotides is extremely long and contains upstream open reading frames, suggesting that translation initiation of ZAS3 transcripts by conventional cap-dependent ribosome scanning mechanism may be inefficient [10].
• Furthermore, in HeLa cells, downregulation of KRC conferred anchorage-independent growth and promoted cell cycle progression without an intervening cytokinesis, culminating in the formation of multinucleated giant cells [8].
• In this report, the function of KRC was studied in a RAG2-deficient blastocyst complementation animal model [3].
• However, by 6 months of age, there was a marked deficit in CD4(+)CD8(+) thymocytes in the chimeras, suggesting that KRC may be involved in T-lymphocyte survival [3].

Associations of HIVEP3 with chemical compounds

• The levels of cationic peroxidase activity and of KRCP transcripts in Korean radish seedlings (Raphanus sativus L. F1 Handsome Fall) were also upregulated by a low ratio of cytokinin to auxin, but not by high concentrations of cytokinin [11].

Regulatory relationships of HIVEP3

• ZAS3 is a large zinc finger protein that regulates kappaB-mediated transcription and TNF-driven signal transduction pathway [10].

Analytical, diagnostic and therapeutic context of HIVEP3

• Tumorigenicity was identified by tumor formation after subcutaneous injections of KRC/Y cells in nude mice, which showed close resemblance to the original tumor by light and electron microscope observations [5].

References