Disease relevance of HLA-DOA

• New HLA DNA polymorphisms associated with autoimmune diseases [1].
• The results indicate that the DRB1*0404 may be associated with risk for the development of gold-induced enterocolitis in this population and suggest that HLA DNA typing should be considered in Jews who may be undergoing chrysotherapy [2].
• The genetic origin of hydatidiform moles was analysed utilizing HLA-DNA typing [3].
• HLA-DNA typing using PCR-SSOP and PCR-DCP methods was performed in 85 patients with Takayasu arteritis and 492 healthy controls who had been typed for HLA by serological method [4].
• A case study of sibling relationship in sarcoidosis by HLA-DNA genotyping [5].

Psychiatry related information on HLA-DOA

• METHODS: HLA-DNA analysis was carried out in 20 healthy Jewish patients (age 23.9+/-6.3 years; 13 Ashkenazi, seven non-Ashkenazi) who had objective measures of hypersomnolence [6].

High impact information on HLA-DOA

• We have isolated two new class II-like complementary DNA sequences, RING6 and RING7, which map between the HLA-DNA and -DOB loci [7].
• One means of overcoming these problems involves a molecular genetic analysis of human lymphoblastoid cell line (LCL) mutants having gamma-ray-induced physical deletions of HLA DNA to map DNA fragments in the MHC [8].
• Polymerase chain reaction showed that peripheral blood mononuclear cells from one subject at different times matched by HLA DNA typing [9].
• To further evaluate the role of DO in Ag processing and presentation, we generated transgenic mice that expressed the human HLA-DOA and HLA-DOB genes under the control of a dendritic cell (DC)-specific promoter [10].
• HLA DNA typing was done on fresh white blood cells from the 4 patients [2].

Biological context of HLA-DOA

• The HLA-DOA gene has been identified in other mammals as well, and the nucleotide sequences were well conserved among these species [11].
• We have previously recognized that all eight of the allelic variations of the HLA-DOA gene represent non-synonymous amino acid substitution [12].
• In this study, we have analyzed genetic polymorphism and allelic variation of the HLA-DOA gene in 37 HLA class II homozygous typing cells using the direct DNA sequencing technique [11].
• To investigate the relationship between meiotic crossover hot spots and block-like linkage disequilibrium (LD), we have extended our high-resolution studies of the human MHC class II region to a 90-kb segment upstream of the HLA-DOA gene [13].
• The HLA-DNA gene of the human major histocompatibility complex (MHC) produces RNA transcripts of unusual size compared to the 1.3 kilobase (kb) transcripts of other class II genes [14].

Anatomical context of HLA-DOA

• Novel polymorphisms in HLA-DOA and HLA-DOB in B-cell malignancies [15].
• The diagnosis was confirmed by HLA-DNA typing with PCR-sequence-specific oligonucleotide that revealed the presence of complex HLA-DR chimerism in the peripheral lymphocytes collected after onset of GVHD [16].
• Parallel technological developments in HLA DNA typing in the clinical laboratory have resulted in a more precise awareness of the role of HLA matching for the classical HLA antigens in bone marrow and solid organ transplantation, while alternative strategies and techniques for donor selection are currently under evaluation [17].
• This method of diagnosis can be combined with genotyping of the fetus by HLA-DNA probes on chorionic villus sampling or can be used alone [18].

Associations of HLA-DOA with chemical compounds

• First trimester prenatal diagnosis of 21-hydroxylase deficiency by linkage analysis to HLA-DNA probes and by 17-hydroxyprogesterone determination [19].

Other interactions of HLA-DOA

• HLA-DO, encoded by the HLA-DOA and HLA-DOB genes, has been shown to function as a modulator of Ag presentation [20].
• Orientation of HLA-DNA gene and identification of a CpG island-associated gene adjacent to DNA in human major histocompatibility complex class II region [21].
• Association between ankylosing spondylitis and a 9.2 kb Pvu II class I HLA DNA restriction fragment: a reassessment [22].
• The ability to type for HLA-DPB alleles routinely by automatic sequence determination and subsequent automated analysis offers new perspectives for HLA-DNA typing [23].
• A system for HLA-DNA typing is described, which uses RFLP patterns generated by sequential hybridization of TaqI-digested DNAs with short DR beta, DQ beta and DQ alpha cDNA probes [24].

Analytical, diagnostic and therapeutic context of HLA-DOA

• HLA DNA typing and transplantation [25].
• Sequence analysis revealed that the H-2Oa gene is the murine equivalent of the human HLA-DNA gene [26].
• The results were compared with those obtained by HLA typing and linkage analysis to a HLA DNA probe [27].
• At months 3 and 4 with donor biopsy specimen class I HLA DNA no longer detectable, immunosuppression was tapered off [28].
• In this study, we performed HLA-DNA typing by PCR to clarify the relationship between AAA and HLA genotypes in Japanese patients with AAA [29].

References