The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

NFATC4  -  nuclear factor of activated T-cells,...

Homo sapiens

Synonyms: NF-AT3, NF-ATC4, NF-ATc4, NFAT3, NFATc4, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of NFATC4

  • Although the strength of the associations was rather weak, these observations raise the hypothesis that the G160A polymorphism of the NFATC4 gene plays a role in the development of human cardiac hypertrophy [1].
  • Knockdown of endogenous NFAT3 reduced the growth of human breast cancer ZR75-1 cells in a ligand-independent manner [2].
  • Transgenic mice that express activated forms of calcineurin or NF-AT3 in the heart develop cardiac hypertrophy and heart failure that mimic human heart disease [3].
  • Previously, we showed that activation of the Ca(2+)/calmodulin-dependent protein phosphatase calcineurin or its target transcription factor NFAT3 was sufficient to evoke myocardial hypertrophy in vivo [4].
  • Nfatc2(-/-) Nfatc4(-/-) mice are also protected from diet-induced obesity [5].

High impact information on NFATC4

  • We show that NFATc4 is specifically expressed in estrogen receptor positive breast cancer cells and inhibits their motility by downregulating the expression of LCN2 [6]
  • We find that mice with disruptions of both NFATc4 and the related NFATc3 genes die around E11 with generalized defects in vessel assembly as well as excessive and disorganized growth of vessels into the neural tube and somites [7].
  • NF-AT3 interacts with the cardiac zinc finger transcription factor GATA4, resulting in synergistic activation of cardiac transcription [3].
  • We show that cardiac hypertrophy is induced by the calcium-dependent phosphatase calcineurin, which dephosphorylates the transcription factor NF-AT3, enabling it to translocate to the nucleus [3].
  • We have isolated two additional members of this gene family, NF-AT3 and NF-AT4, which encode proteins 65% identical to the other NF-AT proteins within the Rel domain [8].
  • Moreover, atrogin-1 attenuates agonist-induced calcineurin activity and represses calcineurin-dependent transactivation and NFATc4 translocation [9].

Chemical compound and disease context of NFATC4

  • NFAT3 belongs to the NFAT family of transcription factors playing important roles in the development of several organ systems and was found to act as a transcriptional coactivator of estrogen receptors (ERalpha and ERbeta) in breast cancer cells [10].

Biological context of NFATC4


Anatomical context of NFATC4


Associations of NFATC4 with chemical compounds

  • A Gly/Ala substitution at position 160 of the NFATC4 protein (G160A) was associated with left ventricular mass and wall thickness (P=0.02 and 0.006, respectively, GA+AA vs GG), the minor allele (Ala) being associated with lower mean values of these parameters [1].
  • NFAT3 increased binding of ERalpha to the estrogen-responsive element and was recruited to endogenous estrogen-responsive promoters [2].
  • We demonstrate here that two transactivation domains, located at the NH(2) and COOH termini of NFATc4, are critical for interacting with CBP [16].
  • Conversely, mutation of the KIX or CH3 domain prevents CBP-mediated potentiation of NFATc4 transcription activation [16].
  • Together, our results demonstrate that COX-2 induction by arsenite is through NFAT3-dependent and AP-1- or NFkappaB-independent pathways and plays a crucial role in antagonizing arsenite-induced cell apoptosis in human bronchial epithelial Beas-2B cells [17].

Physical interactions of NFATC4

  • NFAT3 interacted with ERalpha and ERbeta both in vitro and in mammalian cells in a ligand-independent fashion [2].

Regulatory relationships of NFATC4

  • The knockdown of NFAT3 by its specific small interfering RNA significantly attenuated the silica-induced TNF-alpha transcription [18].
  • Replacement of Ser168 and Ser170, the amino acid residues on which NFAT3 can be phosphorylated, with Ala did not change the ability of NFAT3 to inhibit the transcriptional activity of ERalpha and ERbeta [10].
  • The involvement of Rho-kinase in PE- and 20-HETE-induced NFAT3 translocation in pulmonary artery suggests a level of control not previously recognized in smooth muscle [15].

Other interactions of NFATC4

  • We used a yeast two-hybrid system and identified NFAT3 as a novel ERbeta-binding protein [2].
  • Taken together, these results suggest that NFAT3 may play important roles in ER signaling and represent a novel target for breast cancer therapy [2].
  • Calcineurin, a heterodimeric protein composed of a catalytic and a regulatory subunit, activates the nuclear factor NFATC4 which after translocation to the nucleus associates with the transcription factor GATA4 to activate several cardiac genes involved in hypertrophic response [1].
  • Treatment of granule neurons with Li(+), an inhibitor of the NFAT export kinase GSK3, prevented the nuclear export of NFAT3 and increased granule cell survival even under pro-apoptotic conditions [12].
  • In macrophage-like cells in culture, NFATc1 through NFATc4 are expressed at similar low levels [19].

Analytical, diagnostic and therapeutic context of NFATC4

  • In order to verify the activated calcineurin pathway as described in animal models, we compared the protein expression of NFAT-3 in homogenates within nuclear extracts [20].



  1. Polymorphisms of genes of the cardiac calcineurin pathway and cardiac hypertrophy. Poirier, O., Nicaud, V., McDonagh, T., Dargie, H.J., Desnos, M., Dorent, R., Roizès, G., Schwartz, K., Tiret, L., Komajda, M., Cambien, F. Eur. J. Hum. Genet. (2003) [Pubmed]
  2. Stimulatory cross-talk between NFAT3 and estrogen receptor in breast cancer cells. Zhang, H., Xie, X., Zhu, X., Zhu, J., Hao, C., Lu, Q., Ding, L., Liu, Y., Zhou, L., Liu, Y., Huang, C., Wen, C., Ye, Q. J. Biol. Chem. (2005) [Pubmed]
  3. A calcineurin-dependent transcriptional pathway for cardiac hypertrophy. Molkentin, J.D., Lu, J.R., Antos, C.L., Markham, B., Richardson, J., Robbins, J., Grant, S.R., Olson, E.N. Cell (1998) [Pubmed]
  4. CaM kinase signaling induces cardiac hypertrophy and activates the MEF2 transcription factor in vivo. Passier, R., Zeng, H., Frey, N., Naya, F.J., Nicol, R.L., McKinsey, T.A., Overbeek, P., Richardson, J.A., Grant, S.R., Olson, E.N. J. Clin. Invest. (2000) [Pubmed]
  5. Role of transcription factor NFAT in glucose and insulin homeostasis. Yang, T.T., Suk, H.Y., Yang, X., Olabisi, O., Yu, R.Y., Durand, J., Jelicks, L.A., Kim, J.Y., Scherer, P.E., Wang, Y., Feng, Y., Rossetti, L., Graef, I.A., Crabtree, G.R., Chow, C.W. Mol. Cell. Biol. (2006) [Pubmed]
  6. NFAT3 transcription factor inhibits breast cancer cell motility by targeting the Lipocalin 2 gene. Fougère, M., Gaudineau, B., Barbier, J., Guaddachi, F., Feugeas, J.P., Auboeuf, D., Jauliac, S. Oncogene. (2010) [Pubmed]
  7. Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature. Graef, I.A., Chen, F., Chen, L., Kuo, A., Crabtree, G.R. Cell (2001) [Pubmed]
  8. Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins. Hoey, T., Sun, Y.L., Williamson, K., Xu, X. Immunity (1995) [Pubmed]
  9. Atrogin-1/muscle atrophy F-box inhibits calcineurin-dependent cardiac hypertrophy by participating in an SCF ubiquitin ligase complex. Li, H.H., Kedar, V., Zhang, C., McDonough, H., Arya, R., Wang, D.Z., Patterson, C. J. Clin. Invest. (2004) [Pubmed]
  10. Tissue type-specific modulation of ER transcriptional activity by NFAT3. Zhang, H., Wang, X., Li, J., Zhu, J., Xie, X., Yuan, B., Yang, Z., Zeng, M., Jiang, Z., Li, J., Huang, C., Ye, Q. Biochem. Biophys. Res. Commun. (2007) [Pubmed]
  11. Phosphorylation of NFATc4 by p38 mitogen-activated protein kinases. Yang, T.T., Xiong, Q., Enslen, H., Davis, R.J., Chow, C.W. Mol. Cell. Biol. (2002) [Pubmed]
  12. The transcription factor NFAT3 mediates neuronal survival. Benedito, A.B., Lehtinen, M., Massol, R., Lopes, U.G., Kirchhausen, T., Rao, A., Bonni, A. J. Biol. Chem. (2005) [Pubmed]
  13. NFATc4 and ATF3 Negatively Regulate Adiponectin Gene Expression in 3T3-L1 Adipocytes. Kim, H.B., Kong, M., Kim, T.M., Suh, Y.H., Kim, W.H., Lim, J.H., Song, J.H., Jung, M.H. Diabetes (2006) [Pubmed]
  14. Substance P initiates NFAT-dependent gene expression in spinal neurons. Seybold, V.S., Coicou, L.G., Groth, R.D., Mermelstein, P.G. J. Neurochem. (2006) [Pubmed]
  15. Constrictor-induced translocation of NFAT3 in human and rat pulmonary artery smooth muscle. Yaghi, A., Sims, S.M. Am. J. Physiol. Lung Cell Mol. Physiol. (2005) [Pubmed]
  16. Requirement of two NFATc4 transactivation domains for CBP potentiation. Yang, T., Davis, R.J., Chow, C.W. J. Biol. Chem. (2001) [Pubmed]
  17. Cyclooxygenase-2 induction by arsenite is through a nuclear factor of activated T-cell-dependent pathway and plays an antiapoptotic role in Beas-2B cells. Ding, J., Li, J., Xue, C., Wu, K., Ouyang, W., Zhang, D., Yan, Y., Huang, C. J. Biol. Chem. (2006) [Pubmed]
  18. Essential role of ROS-mediated NFAT activation in TNF-alpha induction by crystalline silica exposure. Ke, Q., Li, J., Ding, J., Ding, M., Wang, L., Liu, B., Costa, M., Huang, C. Am. J. Physiol. Lung Cell Mol. Physiol. (2006) [Pubmed]
  19. NFAT expression in human osteoclasts. Day, C.J., Kim, M.S., Lopez, C.M., Nicholson, G.C., Morrison, N.A. J. Cell. Biochem. (2005) [Pubmed]
  20. Increased regulatory activity of the calcineurin/NFAT pathway in human heart failure. Diedrichs, H., Chi, M., Boelck, B., Mehlhorn, U., Mehlhorm, U., Schwinger, R.H. Eur. J. Heart Fail. (2004) [Pubmed]
WikiGenes - Universities