Disease relevance of OGN

• The polymerase chain reaction was used to obtain clones coding for human OIF from the osteosarcoma cell line, MG-63 [1].

High impact information on OGN

• Implantation of OIF plus transforming growth factor beta (TGF-beta) type 1 or 2 into subcutaneous tissues of rats induces formation of bone at the implantation site [2].
• Osteoinductive factor (OIF) is a glycoprotein in bone that induces ectopic bone formation [2].
• We found that OIF inhibited total multinucleated cell (MNC) formation in a dose-dependent fashion and preferentially inhibited formation of MNCs that react with monoclonal antibody 23c6 (23c6-positive MNCs), an antibody that identifies osteoclasts [2].
• Since TGF-beta is also present in bone matrix and inhibits formation of multinucleated cells that express an osteoclast phenotype in long-term human marrow cultures, we tested the effects of OIF on formation of these osteoclast-like cells to determine the effects of OIF on cells in the osteoclast lineage [2].
• The inhibition of 23c6-positive MNC formation by OIF was not mediated by prostaglandin synthesis [2].

Biological context of OGN

• Assignment of mimecan gene (OGN) to human chromosome band 9q22 by in situ hybridization [3].
• Porcine OGN and ASPN: mapping, polymorphisms and use for quantitative trait loci identification for growth and carcass traits in a Meishan x Piétrain intercross [4].
• Polymorphisms were revealed in both genes, including a pentanucleotide microsatellite (SCZ003) in OGN and two single nucleotide polymorphisms (AM181682.1:g.780G>T and AM181682.1:g.825T>C) in ASPN [4].
• The porcine orthologues of human chromosome HSA9q22.31 genes osteoglycin (OGN) and asporin (ASPN) were mapped to porcine chromosome SSC3 using linkage analysis and a somatic cell hybrid panel [4].
• Epidemiologic perspectives for OGN nursing research [5].

Anatomical context of OGN

• Northern blot analysis of bovine osteoblast mRNA revealed two OIF-specific transcripts of 1.9 and 2.4 kb [1].
• Northern blot analysis of various cell lines and tissues indicated that expression of OIF transcripts is limited and may be restricted to cells of bone lineage [1].
• Proteoglycan-Lb, a small dermatan sulfate proteoglycan expressed in embryonic chick epiphyseal cartilage, is structurally related to osteoinductive factor [6].
• However, osteoglycin mRNA expression was substantially increased in the adventitia and neointima 14 days after balloon injury, implying a role for this protein in vessel remodeling [7].
• Identification of osteoglycin as a component of the vascular matrix. Differential expression by vascular smooth muscle cells during neointima formation and in atherosclerotic plaques [7].

Associations of OGN with chemical compounds

• Although this tissue was found to express lumican and osteoglycin/mimecan, there was little evidence to suggest that these proteoglycans were a major source of the keratan sulfate glycosaminoglycans [8].
• However, the homology analysis revealed that chick PG-Lb showed a higher homology (about 50% in the region containing leucine-rich repeats) to human osteoinductive factor, OIF, rather than to the other small proteoglycans [6].
• Furthermore, 6 cysteine residues are detected in both PG-Lb and OIF with invariant relative positions [6].
• High glucose also induced the secretion of bone morphogenetic protein-2, a known osteoinductive factor, and further increased the secretion normally seen during calcification by 43% at day 7 and 57% at day 14 [9].
• We studied 5,671 cases of medevac reports during Operations Enduring and Iraqi Freedom (OEF and OIF) [10].

Other interactions of OGN

• Interestingly, the expression of several genes, such as osteoglycin, prostate stem cell antigen, and histone deacetylase 3, was variable in the two normal tissues but similar in the cancer tissues [11].

Analytical, diagnostic and therapeutic context of OGN

• Molecular cloning of a novel bone-forming compound: osteoinductive factor [1].
• Sequence analysis of these clones indicated that the 105-amino-acid OIF is synthesized as a larger 299-amino-acid precursor, the carboxyl terminus of which is cleaved to yield the mature protein [1].
• To obtain rapid healing, the expansion space that was created for the positioning of the implant was filled, following the concepts of tissue engineering, with bioactive glass synthetic bone graft material as an osteoconductive factor and autogenous platelet-rich plasma as an osteoinductive factor [12].

References