Disease relevance of LOC511073

• The purification was monitored by a recently developed radioreceptor assay, specific for GIP, using membrane preparations from hamster beta-cell tumors [1].

High impact information on LOC511073

• Fecal consistency was significantly higher during wk 1 of life, and gastrin and glucose-dependent insulinotropic polypeptide increased only on d 1 and/or 2 of life in calves already fed colostrum on d 1, expressing improved functioning of the gastrointestinal tract [2].
• Upon proteolytic digestion of GIP with the staphylococcal V8 protease and with enterokinase, two fragments are formed in each case, corresponding to GIP1-3, GIP4-42, and GIP1-16, GIP17-42, respectively [1].
• The sequence is: Tyr-Ala-Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-Arg- Gln-Gln - Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly-Lys-Lys-Ser-Asp-Trp-Ile-His- Asn-Ile - Thr-Gln, which differs from that of the previously characterized porcine GIP by having isoleucine instead of lysine at position 37 [1].
• By contrast, intraduodenal infusion of 60 mM-HCl caused complete inhibition of gastric emptying, reduction of acid secretion, and an immediate increase in plasma somatostatin from 121.3 +/- 9.4 (S.E.M.) to 286.3 +/- 16.3 pg/ml (P less 0.01) but levels of GIP, insulin, glucagon and glucose were unaltered [3].
• Glucose-dependent insulinotropic polypeptide, growth hormone and cortisol concentrations, and iron intake were low and comparable in both groups and therefore were etiologically not involved in the development of insulin resistance [4].

Biological context of LOC511073

• Plasma concentration and flux rate of palmitate was increased by GIP only at the higher infusion level suggesting lipolysis and possibly fatty acid re-esterification was stimulated at high concentration [5].
• The effects of increasing concentrations (0, 0.1, 1.0, 10.0 nM) of GIP and glucagon-like peptide-1 (GLP-1) on basal and insulin-stimulated lipogenesis in bovine adipose tissue in vitro were investigated after 4 and 24 h of incubation [5].
• By 19 d postpartum, concentrations of GLP-1, GIP, and CCK increased by 2.3-, 1.8-, and 2.8-fold, respectively, compared with values at 11 d before calving [6].
• Effects of transition from late gestation to early lactation on plasma concentrations of glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide 1-(7-36) amide (GLP-1), and cholecystokinin (CCK) have not been reported in cattle [6].

Anatomical context of LOC511073

• We have compared the effects of porcine GIP to that of bovine GIP and bovine GIP(1-39) on glucose-induced insulin secretion from the perfused rat pancreas [7].
• Gastric inhibitory polypeptide (GIP), a hormone secreted by the gastrointestinal tract in response to nutrient absorption, exerts anabolic effects on adipose tissue in some species [5].
• Cells containing immunoreactive GIP were confined to the mucosa of the duodenum, jejunum and ileum and were not observed in the forestomachs, abomasum, large intestine and pancreas [8].
• The objective of the present study was to measure plasma concentrations of GLP-1, GIP, CCK, insulin, glucose, and nonesterified fatty acids in blood plasma obtained from the coccygeal vein of 32 Holstein cows at an average of 11 d before, and 5, 12, and 19 d after calving [6].

Analytical, diagnostic and therapeutic context of LOC511073

• A combination of ion-exchange and reverse-phase high-performance liquid chromatography was used in the isolation which resulted in homogeneous bovine GIP [1].
• A sensitive, precise and specific radioimmunoassay method for measuring plasma gastric inhibitory polypeptide (GIP) is described [9].
• Cells containing gastric inhibitory peptide (GIP) and glucagon immunoreactivity were localized in the alimentary tract of the adult sheep, young lamb, calf and goat kid by indirect immunocytochemistry, using antisera raised to the porcine peptides [8].

References