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Gene Review

GOPC  -  golgi-associated PDZ and coiled-coil motif...

Homo sapiens

Synonyms: CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, GOPC1, ...
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Disease relevance of GOPC


High impact information on GOPC

  • Here we show that Golgi-associated PDZ- and coiled-coil motif-containing protein (GOPC), a recently identified Golgi-associated protein, is predominantly localized at the trans-Golgi region in round spermatids, and male mice in which GOPC has been disrupted are infertile with globozoospermia [5].
  • Thus, GOPC provides important clues to understanding the mechanisms underlying spermatogenesis, and the GOPC-deficient mouse may be a unique and valuable model for human globozoospermia [5].
  • Lack of acrosome formation in mice lacking a Golgi protein, GOPC [5].
  • These data suggest that TC10 regulates both total and plasma membrane CFTR expression by interacting with CAL [6].
  • Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST [7].

Biological context of GOPC

  • In addition, PIST was demonstrated to be involved in autophagy and linked to processes of neurodegeneration [8].
  • Molecular characters and morphological genetics of CAL gene in Chinese cabbage [9].
  • Calsequestrin, a muscle calcium binding protein, has been shown to bind the extracellular matrix protein laminin and evidence has been presented that CAL (initially called aspartactin) is on the cell surface, consistent with a role as a laminin receptor (1) [4].

Anatomical context of GOPC

  • These data reveal that CAL, a novel beta1AR-binding partner, modulates beta1AR intracellular trafficking, thereby revealing a new mechanism of regulation for beta1AR anterograde trafficking through the endoplasmic reticulum-Golgi complex to the plasma membrane [10].
  • Golgin-160 and PIST colocalize to Golgi membranes and interact in vivo [7].
  • The co-expression of hemagglutinin-tagged or Myc-tagged CAL with green fluorescent protein (GFP)-CFTR in COS-7 cells causes a dose-dependent reduction in mature GFP-CFTR, independent of its tags [1].
  • Endogenous SSTR5 in the neuroendocrine AtT-20 tumor cell line is colocalized with PIST in the Golgi apparatus [11].
  • Here, we show that PIST is expressed in pancreatic beta-cells and interacts with SSTR5 in these cells [2].

Associations of GOPC with chemical compounds

  • Mutation of the terminal valine residue resulted in markedly reduced association of the beta1AR-CT with CAL [10].
  • Interestingly, we also discovered a widely expressed isoform of golgin-160, golgin-160B, which lacks the exon encoding the leucine repeat that mediates binding to PIST [7].
  • Importantly, bafilomycin A1 reverses CAL-mediated CFTR degradation [1].
  • Glutathione S-transferase binding experiments identified an internal region of PIST that includes a coiled-coil domain, which interacts directly with golgin-160 [7].
  • CAL is located primarily at the Golgi apparatus, co-localizing with trans-Golgi markers and is sensitive to Brefeldin A treatment [12].

Physical interactions of GOPC

  • The PDZ domain of CAL binds to the C terminus of CFTR [12].
  • The multi-domain protein PIST (protein interacting specifically with Tc10) interacts with the SSTR5 (somatostatin receptor 5) and is responsible for its intracellular localization [2].
  • Here, we report that a Rho family small GTPase TC10 interacts with CAL [6].

Regulatory relationships of GOPC

  • Co-expression of CAL enhances the degradation of CFTR [1].
  • MIN6 cells over-expressing PIST display enhanced glucose-stimulated insulin secretion and a decreased sensitivity to SST-induced inhibition of insulin secretion [2].

Other interactions of GOPC


Analytical, diagnostic and therapeutic context of GOPC


  1. Modulation of mature cystic fibrosis transmembrane regulator protein by the PDZ domain protein CAL. Cheng, J., Wang, H., Guggino, W.B. J. Biol. Chem. (2004) [Pubmed]
  2. The PDZ/coiled-coil domain containing protein PIST modulates insulin secretion in MIN6 insulinoma cells by interacting with somatostatin receptor subtype 5. Wente, W., Efanov, A.M., Treinies, I., Zitzer, H., Gromada, J., Richter, D., Kreienkamp, H.J. FEBS Lett. (2005) [Pubmed]
  3. Gamma heavy chain disease: rapid, sustained response to cyclophosphamide and prednisone. Lyons, R.M., Chaplin, H., Tillack, T.W., Majerus, P.W. Blood (1975) [Pubmed]
  4. Evidence against a laminin receptor role for calsequestrin. Choi, E.S., Sullivan, P.D., Clegg, D.O. Biochem. Biophys. Res. Commun. (1991) [Pubmed]
  5. Lack of acrosome formation in mice lacking a Golgi protein, GOPC. Yao, R., Ito, C., Natsume, Y., Sugitani, Y., Yamanaka, H., Kuretake, S., Yanagida, K., Sato, A., Toshimori, K., Noda, T. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  6. Regulation of cystic fibrosis transmembrane regulator trafficking and protein expression by a Rho family small GTPase TC10. Cheng, J., Wang, H., Guggino, W.B. J. Biol. Chem. (2005) [Pubmed]
  7. Isoform-specific interaction of golgin-160 with the Golgi-associated protein PIST. Hicks, S.W., Machamer, C.E. J. Biol. Chem. (2005) [Pubmed]
  8. CALEB/NGC interacts with the Golgi-associated protein PIST. Hassel, B., Schreff, M., Stube, E.M., Blaich, U., Schumacher, S. J. Biol. Chem. (2003) [Pubmed]
  9. Molecular characters and morphological genetics of CAL gene in Chinese cabbage. Li, X.F., Shen, R.J., Liu, P.L., Tang, Z.C., He, Y.K. Cell Res. (2000) [Pubmed]
  10. Interaction with cystic fibrosis transmembrane conductance regulator-associated ligand (CAL) inhibits beta1-adrenergic receptor surface expression. He, J., Bellini, M., Xu, J., Castleberry, A.M., Hall, R.A. J. Biol. Chem. (2004) [Pubmed]
  11. Interactions with PDZ domain proteins PIST/GOPC and PDZK1 regulate intracellular sorting of the somatostatin receptor subtype 5. Wente, W., Stroh, T., Beaudet, A., Richter, D., Kreienkamp, H.J. J. Biol. Chem. (2005) [Pubmed]
  12. A Golgi-associated PDZ domain protein modulates cystic fibrosis transmembrane regulator plasma membrane expression. Cheng, J., Moyer, B.D., Milewski, M., Loffing, J., Ikeda, M., Mickle, J.E., Cutting, G.R., Li, M., Stanton, B.A., Guggino, W.B. J. Biol. Chem. (2002) [Pubmed]
  13. Solution structure of GOPC PDZ domain and its interaction with the C-terminal motif of neuroligin. Li, X., Zhang, J., Cao, Z., Wu, J., Shi, Y. Protein Sci. (2006) [Pubmed]
  14. Association of the cystic fibrosis transmembrane regulator with CAL: structural features and molecular dynamics. Piserchio, A., Fellows, A., Madden, D.R., Mierke, D.F. Biochemistry (2005) [Pubmed]
  15. Possible interaction of a Rho effector, Rhotekin, with a PDZ-protein, PIST, at synapses of hippocampal neurons. Ito, H., Iwamoto, I., Mizutani, K., Morishita, R., Deguchi, T., Nozawa, Y., Asano, T., Nagata, K. Neurosci. Res. (2006) [Pubmed]
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