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Lig1  -  ligase I, DNA, ATP-dependent

Rattus norvegicus

Synonyms: DNA ligase 1, DNA ligase I
 
 
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Disease relevance of Lig1

  • These results support the idea that, when compared with other DNA ligases, the novel form of DNA ligase has a unique AMP-binding site, may have an absolute requirement for single-strand breaks and, furthermore, may have an altered reaction mechanism to that which is conserved from bacteriophage to mammalian DNA ligase I [1].
  • DNA from T7 phage containing AP (apurinic/apyrimidinic) sites was repaired by the successive actions of three chromatin enzymes [AP endodeoxyribonuclease, DNAase IV (5'----3'-exodeoxyribonuclease) and DNA polymerase-beta] prepared from rat liver and T4-phage DNA ligase [2].
 

High impact information on Lig1

  • In contrast, DNA ligase was not detected on the nuclear matrix even though significant activity was present in isolated nuclei [3].
  • Eukaryotic DNA ligase. Purification and properties of the enzyme from bovine thymus, and immunochemical studies of the enzyme from animal tissues [4].
  • On the other hand the presence of a basal level of DNA ligase I in nondividing cells, strongly suggests an involvement of this enzyme in DNA repair [5].
  • DNA ligase I gene expression during differentiation and cell proliferation [5].
  • The high stability of DNA ligase I mRNA in both resting and proliferating human fibroblasts suggests a cell proliferation dependent rate of transcription [5].
 

Biological context of Lig1

 

Anatomical context of Lig1

  • This conclusion is supported by a threefold increase in DNA ligase I observed 24 h after UV irradiation of human confluent primary fibroblasts [5].
  • A decrease in DNA ligase I mRNA is observed in HL-60 and NIH-3T3 cells after differentiation [5].
  • Nuclear extracts of hepatocytes were separated into soluble and chromatin fractions, and multiple forms of DNA ligase activity were obtained by AcA34 gel filtration chromatography [10].
  • Since DNA ligase is also found in rat liver chromatin, all the activities used for the successful repair in vitro are thus present in the chromatin of a eukaryotic cell [2].
  • To resolve the BER pathway, the activity of essential BER enzymes was examined in mitochondria using oligonucleotide incision assay, DNA polymerase assay, and DNA ligase assay employing specific DNA substrates [11].
 

Associations of Lig1 with chemical compounds

 

Other interactions of Lig1

  • Both rat liver DNA ligases were inhibited by deoxyadenosinetriphosphate, however this inhibition was competitive with respect to ATP, for DNA ligase I (Ki 22 microM) and non-competitive for the 100-kDa DNA ligase (Ki 170 microM) [1].
  • For this to happen, the conditions required are the presence of 5'-PO4 on the downstream primer and supplementation of aging neuronal extracts with DNA-ligase in addition to recombinant DNA polymerase beta [16].
 

Analytical, diagnostic and therapeutic context of Lig1

  • Immunochemical titrations revealed that the increased activity of DNA ligase observed after partial hepatectomy of rat and 16-fold higher activity level of mouse Ehrlich tumor cells compared with the host liver are due to a change in the enzyme quantity but not to a change in the catalytic efficiency of the enzyme molecule [4].
  • We have studied the regulation of mammalian DNA ligase I gene by using a cDNA probe in Northern blot experiments with RNA extracted from several cell types in different growth conditions [5].
  • The presence of convenient restriction sites in the mutagenic primer and in the amplified DNA permit direct replacement of a wild type DNA segment with the mutated segment by treating the PCR mixture with the appropriate restriction endonucleases followed by DNA ligase [17].
  • After SDS-PAGE, such radiolabeled complexes correspond to polypeptides of 130,000 and 80,000 Da for DNA ligase I and to 100,000 Da for DNA ligase II [7].

References

  1. Rat liver DNA ligases. Catalytic properties of a novel form of DNA ligase. Elder, R.H., Montecucco, A., Ciarrocchi, G., Rossignol, J.M. Eur. J. Biochem. (1992) [Pubmed]
  2. Repair of depurinated DNA with enzymes from rat liver chromatin. Goffin, C., Verly, W.G. Biochem. J. (1984) [Pubmed]
  3. Pre-replicative association of multiple replicative enzyme activities with the nuclear matrix during rat liver regeneration. Tubo, R.A., Berezney, R. J. Biol. Chem. (1987) [Pubmed]
  4. Eukaryotic DNA ligase. Purification and properties of the enzyme from bovine thymus, and immunochemical studies of the enzyme from animal tissues. Teraoka, H., Tsukada, K. J. Biol. Chem. (1982) [Pubmed]
  5. DNA ligase I gene expression during differentiation and cell proliferation. Montecucco, A., Biamonti, G., Savini, E., Focher, F., Spadari, S., Ciarrocchi, G. Nucleic Acids Res. (1992) [Pubmed]
  6. The androgenic regulation of the activities of enzymes engaged in the synthesis of deoxyribonucleic acid in rat ventral prostate gland. Rennie, P.S., Symes, E.K., Mainwaring, W.J. Biochem. J. (1975) [Pubmed]
  7. DNA ligases from rat liver. Purification and partial characterization of two molecular forms. Elder, R.H., Rossignol, J.M. Biochemistry (1990) [Pubmed]
  8. Do histones bind to a specific group of DNA sequences in chromatin? A test based on DNA ligase action on reconstituted chromatin. Zimmerman, S.B., Levin, C.J. Biochem. Biophys. Res. Commun. (1975) [Pubmed]
  9. Modulation of carcinogen-induced DNA damage and repair enzyme activity by dietary riboflavin. Webster, R.P., Gawde, M.D., Bhattacharya, R.K. Cancer Lett. (1996) [Pubmed]
  10. DNA ligase activities during hepatocarcinogenesis induced by N-2-acetylaminofluorene. Chan, J.Y., Becker, F.F. Carcinogenesis (1985) [Pubmed]
  11. Detection of DNA base-excision repair activity for oxidative lesions in adult rat brain mitochondria. Chen, D., Lan, J., Pei, W., Chen, J. J. Neurosci. Res. (2000) [Pubmed]
  12. Mammalian DNA ligase. Structure and function in rat-liver tissues. Mezzina, M., Rossignol, J.M., Philippe, M., Izzo, R., Bertazzoni, U., Sarasin, A. Eur. J. Biochem. (1987) [Pubmed]
  13. Modulation by dietary copper of aflatoxin B1-induced activity of DNA repair enzymes poly (ADP-ribose) polymerase, DNA polymerase beta and DNA ligase. Webster, R.P., Gawde, M.D., Bhattacharya, R.K. In Vivo (1996) [Pubmed]
  14. Effect of different vitamin A status on carcinogen-induced DNA damage and repair enzymes in rats. Webster, R.P., Gawde, M.D., Bhattacharya, R.K. In Vivo (1996) [Pubmed]
  15. Induction of DNA ligase during stimulation of DNA synthesis in intact rat liver by a dietary manipulation. Teraoka, H., Okamoto, N., Tamura, S., Tsukada, K. Biochim. Biophys. Acta (1981) [Pubmed]
  16. Reduced DNA gap repair in aging rat neuronal extracts and its restoration by DNA polymerase beta and DNA-ligase. Krishna, T.H., Mahipal, S., Sudhakar, A., Sugimoto, H., Kalluri, R., Rao, K.S. J. Neurochem. (2005) [Pubmed]
  17. Construction of mutant and chimeric genes using the polymerase chain reaction. Vallette, F., Mege, E., Reiss, A., Adesnik, M. Nucleic Acids Res. (1989) [Pubmed]
 
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