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Gene Review

GAL7  -  UDP-glucose:hexose-1-phosphate...

Saccharomyces cerevisiae S288c

Synonyms: Gal-1-P uridylyltransferase, Galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase, YBR018C, YBR0226
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Disease relevance of GAL7


High impact information on GAL7

  • The cloned fragment Sc481 contains coding regions for all or part of three galactose"induced RNAs and may correspond to the GAL 1, GAL 7, GAL 10 gene cluster region of chromosome II [6].
  • The synthesis of heat-shock-inducible HSP26 mRNA and galactose-inducible GAL7 and GAL10 mRNAs is also drastically inhibited in the rad3-ts mutant at the restrictive temperature [7].
  • A strain carrying a plasmid with the 35S rRNA coding region fused to the GAL7 promoter and transcribed by Pol II contained a rounded nucleolus that often lacked extensive contact with the nuclear envelope [8].
  • The sequence was located 4-5 nucleotides upstream from the 3' end, i.e. the polyadenylation site, of the GAL7 mRNA [9].
  • In vivo footprinting data reveal that GAL7 promoter occlusion is associated with the displacement of Gal4p transcription factors from the promoter [10].

Biological context of GAL7

  • Expression of the Saccharomyces cerevisiae GAL7 gene on autonomous plasmids [11].
  • The induction kinetics and final accumulation of the chromosomal GAL10 mRNA were also affected by the presence of multiple copies of the GAL7 gene; these results are consistent with a model involving limiting amounts of regulatory factors [11].
  • Increasing the gene dosage to more than 200 copies per cell resulted in constitutive expression of the GAL7 mRNA; fully induced mRNA levels were increased more than 10-fold at these high gene dosages [11].
  • Normal expression of GAL7 could occur in the absence of DNA encoding the functional genes of the GAL cluster region and was not altered when the gene was adjacent to other plasmid elements such as autonomously replicating sequences or centromeres [11].
  • We investigated the genetic basis of this constitutive gene expression and found no recombinants between the constitutive and Gal- phenotypes among 76 tetrads, suggesting that either GAL7 or a tightly linked gene codes for a regulatory function [12].

Anatomical context of GAL7


Associations of GAL7 with chemical compounds

  • The chromosomal and single-copy centromeric plasmid locations of GAL7 were indistinguishable in their response to growth conditions (induction by galactose, repression by glucose) and positive and negative regulatory factors (GAL4 and GAL80) [11].
  • We present the nucleotide sequence of a 1599-base pair (bp) DNA fragment containing the entire GAL7 gene that encodes galactose-1-phosphate uridyltransferase of Saccharomyces cerevisiae [14].
  • Poly(A) signals control both transcriptional termination and initiation between the tandem GAL10 and GAL7 genes of Saccharomyces cerevisiae [15].
  • Our observation of strand-selective repair of thymine glycols in the GAL7 gene is the first evidence that this repair process occurs for a nonbulky lesion [16].
  • We examined the role of transcription in directing repair of DNA damage in active genes by comparing the repair of thymine glycols produced by H2O2 and of UV-induced pyrimidine dimers on each strand of the GAL7 gene of Saccharomyces cerevisiae [16].

Physical interactions of GAL7


Regulatory relationships of GAL7

  • Analysis with host regulatory mutants delta gal14 and delta gal180 suggests that these sequences are the site at which the GAL4 product exerts its action to activate the GAL7 gene [2].

Other interactions of GAL7

  • About 5 kilobases of the sequence was determined, which includes the entire GAL1 gene, the two intercistronic regions, and portions of the coding sequences of GAL10 and GAL7 [18].
  • Transcription of multiple copies of the yeast GAL7 gene is limited by specific factors in addition to GAL4 [19].
  • In experiments in which the presence of either the plasmid-carried cloned GAL3 gene or the plasmid-carried cloned GAL1-10-7 genes allows MEL1 induction of a gal3 gal1 gal7 cell, we find that loss of the plasmid results in the shutoff of MEL1 expression even when galactose is continuously present [20].
  • Moreover, expression of UGP1 allows a gal7 strain to grow on galactose as a sole carbon source [13].
  • Nonetheless, essential signals have seemed to be confined to compact regions in vivo, and we find that a short RNA with only 70 bases of GAL7 sequence upstream and 8 to 10 bases downstream of the poly(A) addition site is processed in vitro, as is an analogous CYC1 pre-RNA [21].

Analytical, diagnostic and therapeutic context of GAL7


  1. Multiple copies of MRG19 suppress transcription of the GAL1 promoter in a GAL80-dependent manner in Saccharomyces cerevisiae. Kabir, M.A., Khanday, F.A., Mehta, D.V., Bhat, P.J. Mol. Gen. Genet. (2000) [Pubmed]
  2. Duplicate upstream activating sequences in the promoter region of the Saccharomyces cerevisiae GAL7 gene. Tajima, M., Nogi, Y., Fukasawa, T. Mol. Cell. Biol. (1986) [Pubmed]
  3. Expression of the human adenovirus E1a product in yeast. Handa, H., Toda, T., Tajima, M., Wada, T., Iida, H., Fukasawa, T. Gene (1987) [Pubmed]
  4. Production of polyomavirus structural protein VP1 in yeast cells and its interaction with cell structures. Palková, Z., Adamec, T., Liebl, D., Stokrová, J., Forstová, J. FEBS Lett. (2000) [Pubmed]
  5. Functional consequence of substitutions at residue 171 in human galactose-1-phosphate uridylyltransferase. Crews, C., Wilkinson, K.D., Wells, L., Perkins, C., Fridovich-Keil, J.L. J. Biol. Chem. (2000) [Pubmed]
  6. Isolation of galactose-inducible DNA sequences from Saccharomyces cerevisiae by differential plaque filter hybridization. St John, T.P., Davis, R.W. Cell (1979) [Pubmed]
  7. DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase II. Guzder, S.N., Qiu, H., Sommers, C.H., Sung, P., Prakash, L., Prakash, S. Nature (1994) [Pubmed]
  8. Mutational analysis of the structure and localization of the nucleolus in the yeast Saccharomyces cerevisiae. Oakes, M., Aris, J.P., Brockenbrough, J.S., Wai, H., Vu, L., Nomura, M. J. Cell Biol. (1998) [Pubmed]
  9. Signal sequence for generation of mRNA 3' end in the Saccharomyces cerevisiae GAL7 gene. Abe, A., Hiraoka, Y., Fukasawa, T. EMBO J. (1990) [Pubmed]
  10. Balancing transcriptional interference and initiation on the GAL7 promoter of Saccharomyces cerevisiae. Greger, I.H., Aranda, A., Proudfoot, N. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  11. Expression of the Saccharomyces cerevisiae GAL7 gene on autonomous plasmids. Baker, S.M., Okkema, P.G., Jaehning, J.A. Mol. Cell. Biol. (1984) [Pubmed]
  12. Genetic and biochemical characterization of the galactose gene cluster in Kluyveromyces lactis. Riley, M.I., Dickson, R.C. J. Bacteriol. (1984) [Pubmed]
  13. Expression of human inositol monophosphatase suppresses galactose toxicity in Saccharomyces cerevisiae: possible implications in galactosemia. Mehta, D.V., Kabir, A., Bhat, P.J. Biochim. Biophys. Acta (1999) [Pubmed]
  14. Primary structure of the Saccharomyces cerevisiae GAL7 gene. Tajima, M., Nogi, Y., Fukasawa, T. Yeast (1985) [Pubmed]
  15. Poly(A) signals control both transcriptional termination and initiation between the tandem GAL10 and GAL7 genes of Saccharomyces cerevisiae. Greger, I.H., Proudfoot, N.J. EMBO J. (1998) [Pubmed]
  16. Strand-selective repair of DNA damage in the yeast GAL7 gene requires RNA polymerase II. Leadon, S.A., Lawrence, D.A. J. Biol. Chem. (1992) [Pubmed]
  17. A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeast. Lorch, Y., Kornberg, R.D. J. Mol. Biol. (1985) [Pubmed]
  18. Sequence of the Saccharomyces GAL region and its transcription in vivo. Citron, B.A., Donelson, J.E. J. Bacteriol. (1984) [Pubmed]
  19. Transcription of multiple copies of the yeast GAL7 gene is limited by specific factors in addition to GAL4. Baker, S.M., Johnston, S.A., Hopper, J.E., Jaehning, J.A. Mol. Gen. Genet. (1987) [Pubmed]
  20. Genetic and molecular analysis of the GAL3 gene in the expression of the galactose/melibiose regulon of Saccharomyces cerevisiae. Torchia, T.E., Hopper, J.E. Genetics (1986) [Pubmed]
  21. Unusual aspects of in vitro RNA processing in the 3' regions of the GAL1, GAL7, and GAL10 genes in Saccharomyces cerevisiae. Sadhale, P.P., Platt, T. Mol. Cell. Biol. (1992) [Pubmed]
  22. Unique distribution of GAL genes on chromosome XI in the yeast Saccharomyces naganishii. Kodama, T., Hisatomi, T., Kakiuchi, M., Aya, R., Yoshida, K., Bando, Y., Takami, T., Tsuboi, M. Curr. Microbiol. (2003) [Pubmed]
  23. Polymerase chain reaction mapping of yeast GAL7 mRNA polyadenylation sites demonstrates that 3' end processing in vitro faithfully reproduces the 3' ends observed in vivo. Sadhale, P.P., Sapolsky, R., Davis, R.W., Butler, J.S., Platt, T. Nucleic Acids Res. (1991) [Pubmed]
  24. Constitutive expression in gal7 mutants of Kluyveromyces lactis is due to internal production of galactose as an inducer of the Gal/Lac regulon. Cardinali, G., Vollenbroich, V., Jeon, M.S., de Graaf, A.A., Hollenberg, C.P. Mol. Cell. Biol. (1997) [Pubmed]
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