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Gene Review

MET2  -  homoserine O-acetyltransferase

Saccharomyces cerevisiae S288c

Synonyms: Homoserine O-acetyltransferase, Homoserine O-trans-acetylase, N0615, YNL277W
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Disease relevance of MET2


High impact information on MET2

  • The activation region contained a repeated dyad sequence that is also found in the promoter regions of other unlinked but coordinately regulated genes (MET3, MET2, and SAM2) [2].
  • This finding indicated that met2 genes were silenced by methylation alone [3].
  • In 70% of the double-stranded-cut DNA transformants, one or more copies of the transforming DNA had integrated at the met2 locus, leading to tandem duplications of the met2 target region separated by plasmid DNA [3].
  • Circular single-stranded DNA carrying the met2 gene and double-stranded DNA linearized by cutting within the met2 gene both transformed protoplasts of a met2 mutant strain of A. immersus to prototrophy [3].
  • Electrophoretic karyotyping analyses, restriction fragment length polymorphism maps of PCR-amplified MET2 gene fragments, and the sequence analysis of a part of the two MET2 gene alleles found support the notion that these two strains constitute hybrids between Saccharomyces cerevisiae and Saccharomyces bayanus [4].

Biological context of MET2


Associations of MET2 with chemical compounds


Other interactions of MET2

  • Further analysis carried out with three other markers, BAP2, HO and MET2 showed that they have also diverged from their S. uvarum counterparts by MNMA [12].
  • Partial DNA sequencing of tagged genes showed that they were homologous to the S. cerevisiae genes RIB1, MET2, and SEF1 [13].
  • On the basis of these results, we conclude that met2 and met17 (met15) cause accumulation of hydrosulfide ions in the cell and that the increased level of hydrosulfide is responsible for detoxification of methylmercury [1].

Analytical, diagnostic and therapeutic context of MET2

  • Using Southern hybridization with species-specific molecular markers, RFLP of the MET2 gene and flow cytometry analysis, we showed that the non-S. cerevisiae parents are different in lager brewing yeasts and in wine hybrid strains [14].
  • Molecular cloning and regulation of the expression of the MET2 gene of Saccharomyces cerevisiae [9].
  • A polyadenylated mRNA of 1700 nt is detected by Northern blot hybridization with a MET2 probe [9].


  1. Role of hydrosulfide ions (HS-) in methylmercury resistance in Saccharomyces cerevisiae. Ono, B., Ishii, N., Fujino, S., Aoyama, I. Appl. Environ. Microbiol. (1991) [Pubmed]
  2. Elements involved in S-adenosylmethionine-mediated regulation of the Saccharomyces cerevisiae MET25 gene. Thomas, D., Cherest, H., Surdin-Kerjan, Y. Mol. Cell. Biol. (1989) [Pubmed]
  3. Targeted transformation of Ascobolus immersus and de novo methylation of the resulting duplicated DNA sequences. Goyon, C., Faugeron, G. Mol. Cell. Biol. (1989) [Pubmed]
  4. New hybrids between Saccharomyces sensu stricto yeast species found among wine and cider production strains. Masneuf, I., Hansen, J., Groth, C., Piskur, J., Dubourdieu, D. Appl. Environ. Microbiol. (1998) [Pubmed]
  5. Aerobic isolation of an ERG24 null mutant of Saccharomyces cerevisiae. Crowley, J.H., Smith, S.J., Leak, F.W., Parks, L.W. J. Bacteriol. (1996) [Pubmed]
  6. Genetic reidentification of the pectinolytic yeast strain SCPP as Saccharomyces bayanus var. uvarum. Naumov, G.I., Naumova, E.S., Aigle, M., Masneuf, I., Belarbi, A. Appl. Microbiol. Biotechnol. (2001) [Pubmed]
  7. Efficient selection of hybrids by protoplast fusion using drug resistance markers and reporter genes in Saccharomyces cerevisiae. Nakazawa, N., Iwano, K. J. Biosci. Bioeng. (2004) [Pubmed]
  8. The MET2 gene of Saccharomyces cerevisiae: molecular cloning and nucleotide sequence. Langin, T., Faugeron, G., Goyon, C., Nicolas, A., Rossignol, J.L. Gene (1986) [Pubmed]
  9. Molecular cloning and regulation of the expression of the MET2 gene of Saccharomyces cerevisiae. Baroni, M., Livian, S., Martegani, E., Alberghina, L. Gene (1986) [Pubmed]
  10. Saccharomyces carlsbergensis contains two functional MET2 alleles similar to homologues from S. cerevisiae and S. monacensis. Hansen, J., Kielland-Brandt, M.C. Gene (1994) [Pubmed]
  11. Inactivation of MET2 in brewer's yeast increases the level of sulfite in beer. Hansen, J., Kielland-Brandt, M.C. J. Biotechnol. (1996) [Pubmed]
  12. Evolutionary relationships between the former species Saccharomyces uvarum and the hybrids Saccharomyces bayanus and Saccharomyces pastorianus; reinstatement of Saccharomyces uvarum (Beijerinck) as a distinct species. Nguyen, H.V., Gaillardin, C. FEMS Yeast Res. (2005) [Pubmed]
  13. Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments. Dmytruk, K.V., Voronovsky, A.Y., Sibirny, A.A. Curr. Genet. (2006) [Pubmed]
  14. Molecular genetic study of introgression between Saccharomyces bayanus and S. cerevisiae. Naumova, E.S., Naumov, G.I., Masneuf-Pomarède, I., Aigle, M., Dubourdieu, D. Yeast (2005) [Pubmed]
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