Disease relevance of Clonorchis sinensis

• A gene encoding cysteine proteinase from Clonorchis sinensis has been cloned and expressed in Escherichia coli [1].
• A counterimmunoelectrophoresis test was used to detect antibodies against the adult worm antigen of Clonorchis sinensis in sera from 70 clonorchiasis patients, 20 uninfected healthy persons and 7 patients infected with other helminths [2].
• The antibody test (ELISA) was evaluated for its significance by reacting human sera from clinically diagnosed metagonimiasis, fascioliasis, clonorchiasis and paragonimiasis with 4 crude extracts of Metagonimus yokogawai (metacercariae), adults of Fasciola hepatica, Clonorchis sinensis and Paragonimus westermani [3].

High impact information on Clonorchis sinensis

• Heterogeneity of the "oval-cell" response in the hamster liver during cholangiocarcinogenesis following Clonorchis sinensis infection and dimethylnitrosamine treatment [4].
• Vaccination with DNA encoding cysteine proteinase confers protective immune response to rats infected with Clonorchis sinensis [5].
• Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis [6].
• A copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized [6].
• A novel 28 kDa cysteine protease (Cs28CF) secreted by the hepatobiliary trematode, Clonorchis sinensis was identified [7].

Biological context of Clonorchis sinensis

• METHODS: The origin and fate of small "oval" cells expressing different immunohistologic phenotypes and ultrastructural appearance were examined in livers of Syrian hamsters during cholangiocarcinogenesis induced by dimethylnitrosamine and promoted by Clonorchis sinensis infection [4].

Anatomical context of Clonorchis sinensis

• We performed bromodeoxyuridine (BrdU) staining to observe the proliferation pattern of epithelial cells on the biliary mucosa in Clonorchis sinensis infection [8].

Associations of Clonorchis sinensis with chemical compounds

• Crystallization of Clonorchis sinensis 26 kDa glutathione S-transferase and its fusion proteins with peptides of different lengths [9].
• A Clonorchis sinensis 26 kDa glutathione S-transferase (CsGST) and its fusion proteins containing 14 and 48 amino-acid peptides at the N-terminus have been crystallized using polyethylene glycol monomethylether 550 as a precipitant [9].
• A 7-kDa protein was purified from extracts of adult Clonorchis sinensis by a combination of ammonium sulfate precipitation, anion exchange chromatography, cation exchange chromatography, gel-filtration chromatography, and reversed-phase FPLC [10].
• In the two cases where ghosts of degenerated cells and partially preserved liver reticulin pattern were noted, worms were identified, one being Clonorchis sinensis [11].
• Studies on the use of cobalt-60 gamma irradiation to control infectivity of Clonorchis sinensis metacercariae [12].

Gene context of Clonorchis sinensis

• CONCLUSION: Clonorchis sinensis infection is related to aberrant COX-2 expression in patients with CC [13].
• Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis [14].
• A cathepsin F of adult Clonorchis sinensis and its phylogenetic conservation in trematodes [7].
• Metacercariae of Clonorchis sinensis were collected from freshwater fish (Pseudorasbora parva) after storage in a freezer at -12 degrees C for 10-20 days, in a freezer at -20 degrees for 3-7 days, or treating with heavy salt (fish/salt = 10 gm/3 gm) at 26 degrees for 5-15 days [15].
• Cloning and characterization of Clonorchis sinensis myoglobin using immune sera against excretory-secretory antigens [16].

Analytical, diagnostic and therapeutic context of Clonorchis sinensis

• Molecular cloning and characterization of a mu-class glutathione S-transferase from Clonorchis sinensis [17].
• A 28-kDa glutathione S-transferase (Cs28GST) was purified from a Clonorchis sinensis cytosolic fraction through anion-exchange and glutathione-affinity column chromatographies [18].
• Sera of 31 patients infected with Clonorchis sinensis were examined using fraction 1 antigen by ELISA during a one-year observation [19].
• The most closely-related GST of OV28GST among those published for trematodes is a 28 kDa GST of Clonorchis sinensis as shown by multiple sequence alignment and phylogenetic analysis [20].

References