Disease relevance of Fasciola

• The aa sequence of Sj-FABPC exhibits 91% identity to a FABP of S. mansoni (Sm14) and 45% identity to a FABP of Fasciola hepatica (Fh15), putative vaccine candidates for schistosomiasis [1].
• Proline in fascioliasis: III. Activities of pyrroline-5-carboxylic acid reductase and pyrroline-5-carboxylic acid dehydrogenase in Fasciola [2].
• A study was conducted to understand pharmacokinetics and flukicidal activity of intraruminal administration of triclabendazole (TCBZ) at 12.0, 24.0 and 36.0 mg kg-1 body weight in experimentally Fasciola gigantica-infected buffaloes on Week 2 and 10 post-infection [3].
• The sensitivity of the test in experimentally and naturally infected goats was 57.1 and 52.5%, respectively, whereas its specificity using antisera raised against T. solium cysticercosis, hydatid cyst and Fasciola gigantica was 66.7 and 83.4% with partially purified and fractionated antigens, respectively [4].
• Antiphospholipid syndrome was secondary to systemic lupus erythematosus in two, to Fasciola hepatica infection in one and was considered as primary in two [5].

High impact information on Fasciola

• In Fasciola hepatica these receptors are coupled to an adenylate cyclase through a cellular component that requires guanosine triphosphate [6].
• The secretion and activation of the major cathepsin L1 cysteine protease involved in the virulence of the helminth pathogen Fasciola hepatica was investigated [7].
• We have identified a new spliced leader (SL) in Fasciola hepatica by characterizing the 5'-terminal sequences of its enolase mRNA, an mRNA also trans-spliced in the flatworm Schistosoma mansoni (Rajkovic, A., Davis, R.E., Simonsen, J.N., and Rottman, F.M. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 8879-8883) [8].
• The glutathione S-transferases (FhGST) of the liver fluke Fasciola hepatica have been identified as novel vaccine candidates that protect sheep against a fluke infection [9].
• Serotonin-activated adenylate cyclase from Fasciola hepatica. Properties of the solubilized enzyme [10].

Chemical compound and disease context of Fasciola

• Immunodiagnosis of Fasciola hepatica infection (fascioliasis) in a human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase [11].
• The effects of a subclinical fascioliasis at various stages of its development (at week 3, 6 and 9 after infection by oral administration of 20 metacercariae of Fasciola hepatica) in rats were determined on the activity of enzymes involved in liver metabolism of glutathione and on the subunit pattern of cytosolic glutathione S-transferase [12].
• Three-month-old, Polled Dorset rams infected with Fasciola hepatica and uninfected control rams were monitored throughout the peripuberal phase for peripheral concentrations of testosterone and for body weight [13].
• Toxicity of fluometuron and diuron on the intermediate snail host (Lymnea spp.) of Fasciola hepatica [14].
• Fascioliasis of livestock and snail host for Fasciola in the Altiplano Region of Bolivia [15].

Biological context of Fasciola

• The in vivo effects of rafoxanide on the energy metabolism of Fasciola hepatica [16].
• Degenerate oligonucleotide primers derived from conserved cysteine protease sequences were used in the reverse transcription polymerase chain reaction to amplify seven different cysteine protease cDNA clones, Fcp1-7, from RNA isolated from adult Fasciola hepatica [17].
• Developmental expression of a Fasciola hepatica sequence homologous to ABC transporters [18].
• Cell mediated immunity (CMI) to Fasciola hepatica antigens was detected by lymphocyte proliferation and interleukin-2 (IL-2) production tests in cattle during the first 4 weeks following liver fluke infection [19].
• The amino acid sequence of Cs28 showed 60% homology with the vitelline B precursor protein of Opisthorchis viverrini and of 33% homology with the vitelline B1 and B2 proteins of Fasciola hepatica [20].

Anatomical context of Fasciola

• Thioredoxin peroxidase secreted by Fasciola hepatica induces the alternative activation of macrophages [21].
• Basic fibroblast growth factor immunohistochemistry revealed on control brains the protein in the nuclei of glial cells throughout the forebrain and the midbrain and in the nuclei of neurons of the layer II of the retrosplenial granular cortex, the CA2 region of the hippocampus and the fasciola cinereum as well as in the nuclei of ependymal cells [22].
• 5-HT is a candidate for the excitatory transmitter at the neuromuscular junction in trematodes including Fasciola hepatica [23].
• The amount of 5-HT fibers was significantly lower in CA4 and fasciola cinereum of the dorsal hippocampus, caudate-putamen, and hypothalamus of the P as compared with NP rats (unpaired Student's t tests) [24].
• In the hippocampus, AMGBi was densest in the fasciola cinerum, polymorph cells of the dentate gyrus, and pyramidal cell layer of the CA3 regions [25].

Associations of Fasciola with chemical compounds

• Adenosine cyclic 3',5'-monophosphate in the liver fluke, Fasciola hepatica. II. Activation of protein kinase by 5-hydroxytryptamine [26].
• The secreted cathepsin L-like proteinases of the trematode, Fasciola hepatica, contain 3-hydroxyproline residues [27].
• This protein, which represents 6-7% of the total protein in mature Fasciola, is unique in containing rather high levels of the amino acid 3,4-dihydroxyphenylalanine (DOPA), i.e., 110 residues per 1000 [28].
• A histidine-rich protein from the vitellaria of the liver fluke Fasciola hepatica [29].
• Imported Fasciola hepatica infection in the United States and treatment with triclabendazole [30].

Gene context of Fasciola

• Mucin-type O-glycosylation in Fasciola hepatica: characterisation of carcinoma-associated Tn and sialyl-Tn antigens and evaluation of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase activity [31].
• Expression of interleukin 4, interleukin 4 splice variants and interferon gamma mRNA in calves experimentally infected with Fasciola hepatica [32].
• Identification of enolase as a plasminogen-binding protein in excretory-secretory products of Fasciola hepatica [33].
• In addition, high level secretion of IL-4 by Fasciola hepatica specific Th2 clones, but not by a Babesia bovis specific Th1 clone, was confirmed [34].
• Acyl-CoA transferase activities are present in crude homogenates of Fasciola, which could account for the synthesis of succinyl-CoA from succinate by the transfer of CoA from either propionyl-CoA or acetyl-CoA [35].

Analytical, diagnostic and therapeutic context of Fasciola

• Crystallization, structural determination and analysis of a novel parasite vaccine candidate: Fasciola hepatica glutathione S-transferase [36].
• Molecular cloning and expression of Cu/Zn-containing superoxide dismutase from Fasciola hepatica [37].
• Serodiagnosis of human fascioliasis by a cystatin capture enzyme-linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen [38].
• A proteolytic enzyme of the liver fluke Fasciola sp. was purified as described previously by ammonium sulfate fractionation, gel filtration on Sephadex G-200 column and L-phenylalanine-agarose chromatography [39].
• The effect of the deacetylated (amine) metabolite of diamphenethide (DAMD, 10 micrograms ml-1) on the uptake and incorporation by adult Fasciola hepatica of radioactively labelled precursors of DNA, RNA and protein synthesis ([3H]thymidine, [3H]uridine and [3H]leucine, respectively) was measured by liquid scintillation counting [40].

References