Disease relevance of Ranunculaceae

• The cytosolic superoxide dismutase (SOD) of Fasciola hepatica, a causative agent of fascioliasis, was purified and characterized [1].
• By contrast, the Th1 response to B. pertussis antigens was markedly suppressed and the bacterial infection was exacerbated following infection with F. hepatica [2].
• These experiments demonstrate that F. hepatica glycoprotein antigens induce in mice significant protection to infection with S. mansoni and offer an interesting approach to the study of vaccines in experimental schistosomiasis [3].
• Fasciola hepatica suppresses a protective Th1 response against Bordetella pertussis [2].
• Sera from individuals with schistosomiasis mansoni or trichinosis or from normal controls did not recognize the 17-kDa F. hepatica antigenic polypeptide [4].

High impact information on Ranunculaceae

• Vaccination of sheep against Fasciola hepatica with glutathione S-transferase. Identification and mapping of antibody epitopes on a three-dimensional model of the antigen [5].
• The antibody was able to elicit passive cutaneous anaphylaxis in the rat skin against Sj with the highest titer of 1/256,000 in an ascitic form but did not cross-react with any of antigens extracted from S. mansoni, Fasciola hepatica, Paragoniumus westermani, or Trichinella spiralis [6].
• 5-Hydroxytryptamine and motility in Fasciola hepatica [7].
• Adonis aestivalis (Ranunculaceae) is unusual among plants in synthesizing and accumulating large amounts of astaxanthin and other ketocarotenoids [8].
• Triclabendazole and bithionol are the most effective drugs against F. hepatica [9].

Chemical compound and disease context of Ranunculaceae

• Cathepsin L1, a cysteine protease secreted by the gastrodermis of juvenile and adult Fasciola hepatica, was expressed in Escherichia coli as a fusion protein containing the proregion, supplied with six histidyl residues at the N-terminal end (rproCL1) [10].
• The anthelmintic efficacy of albendazole was evaluated as an oral drench at dosages of 15.0, 10.0, and 7.5 mg/kg of body weight in 3 groups crossbred Brahman calves (n = 12 group) infected with Fasciola hepatica [11].
• In limited trials against experimentally induced Fasciola hepatica infections, diamfenetide (N,N'-[oxybis(2,1-ethanediyloxy-4,1-phenylene)]bisacetamide) showed potential value for the prevention of acute fascioliasis in sheep and cattle [12].
• A new fasciolicide, triclabendazole, tested at single oral dose rates of 5 and 10 mg/kg body-weight, proved to be highly effective (greater than 99%) against chronic field infections of Fasciola hepatica and F. gigantica in sheep [13].
• The anthelmintic efficacy of mebendazole against adult Fasciola hepatica and a concurrent mixed nematode infection in sheep [14].

Biological context of Ranunculaceae

• Beneficial effect of dexamethasone on attenuated hormone-induced uptake of calcium and glycogenolysis by perfused liver of rats infected with Fasciola hepatica [15].
• The marked decrease in ATP and glycogen, and the increase in total [NAD+]/[NADH] and [oxaloacetate]/[malate], together with the changed content of other metabolites, led to the conclusion that the mode of action of rafoxanide against F. hepatica in vivo is by uncoupling oxidative phosphorylation [16].
• Fasciola hepatica adult worm cysteine proteinases were active-site, affinity radio-labeled with benzyloxicarbonyl-L-tyrosine-L-alanine diazomethylketone (Z-Tyr125I-Ala-CHN2) [17].
• In vivo regulation of phosphorylation level and activity of phosphofructokinase by serotonin in Fasciola hepatica [18].
• This report demonstrates the essential function of RQ in anaerobic energy metabolism during the entire life cycle of Fasciola hepatica, as the amount of RQ present reflected the importance of fumarate reduction in various stages [19].

Anatomical context of Ranunculaceae

• This study reports that bovine IL-10 mRNA is expressed by Th0, Th1, and Th2 clones of bovine T cells specific for either Babesia bovis or Fasciola hepatica but not by two CD8+ T-cell clones [20].
• Our data suggest that the Th2 responses induced by the helminth F. hepatica are mediated through the secretion of molecules, one of which is TPx, that induce the recruitment and alternative activation of macrophages [21].
• Sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis of [3H]LSD labeled membranes from F. hepatica showed two proteins which were selectively labeled by the photoreactive [3H]LSD [22].
• Fasciola hepatica: the effect of the microtubule inhibitors colchicine and tubulozole-C on the ultrastructure of the adult fluke [23].
• Protection of Fasciola hepatica in the gut mucosa of immune rats is associated with infiltrates of eosinophils, IgG1 and IgG2a antibodies around the parasites [24].

Associations of Ranunculaceae with chemical compounds

• Glutathione S-transferase. Novel vaccine against Fasciola hepatica infection in sheep [25].
• Adenylate cyclase from Fasciola hepatica. 1. Ligand specificity of adenylate cyclase-coupled serotonin receptors [26].
• Adenylate cyclase from Fasciola hepatica. 2. Role of guanine nucleotides in coupling adenylate cyclase and serotonin receptors [27].
• AGA and AGG are found in the F. hepatica mitochondrial protein genes and both codons appear to specify serine [28].
• Effect of some phosphodiesterase inhibitors on adenylate cyclase from the liver fluke, Fasciola hepatica [29].

Gene context of Ranunculaceae

• Cloning of peroxiredoxin, a novel antioxidant enzyme, from the helminth parasite Fasciola hepatica [30].
• Since a human IL-4delta2 was assumed to act as competitive inhibitor of IL-4, it was important to know whether expression of these splice variants of bovine IL-4 have a regulatory function during an immune response to infection with F. hepatica [31].
• Interestingly, PBMCs responded to stimulation with F. hepatica secretory-excretory products (FhSEP) already on PID 10 and expressed high amounts of IL-4 but not of IFN-gamma mRNA suggesting that F. hepatica induced a Th2 biased early immune response which was not restricted to the site of infection [31].
• Inhibition of COXs and 5-LOX and activation of PPARs by Australian Clematis species (Ranunculaceae) [32].
• Mice with a coinfection of F. hepatica and B. pertussis exhibited a Th2 cytokine profile in response to F. hepatica antigens, similar to those infected with F. hepatica alone [2].

Analytical, diagnostic and therapeutic context of Ranunculaceae

• A diagnostic ELISA with recombinant Fasciola hepatica cathepsin L-like protease as antigen was developed to detect antibodies against F. hepatica in sheep and cattle [33].
• This DNP-labeled peptide, DNP-Ahx-Gly-Phe-Ala-CHN2, was used to detect a 30-kDa cathepsin L-like protease, produced by Fasciola hepatica, on Western blots with high sensitivity and relatively low background staining [34].
• Sequence analysis of a Fasciola hepatica glutathione S-transferase cDNA clone [35].
• The head region of the trematode parasite Fasciola hepatica contains 3.47 +/- 0.42 pmol/mg wet wt. of serotonin as measured by high-performance liquid chromatography coupled to electrochemical detection [36].
• Total RNA was extracted from mature and juvenile Fasciola hepatica by homogenizing in 5.0 M guanidine thiocyanate and centrifugation through a 5.7 M CsCl cushion [37].

References