Disease relevance of (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium

• Acute lymphoblastic leukemia with brilliant cresyl blue erythrocytic inclusions--acquired hemoglobin H [1]?
• Usefulness of brilliant cresyl blue staining as an auxiliary method of screening for alpha-thalassemia [2].
• The brilliant cresyl blue test reliably detects couples at risk for hemoglobin Bart's hydrops fetalis [3].
• Effect of brilliant cresyl blue on erythrocytes in hereditary elliptocytosis [4].
• To evaluate the effectiveness of this method, we used brilliant cresyl blue (BCB) staining of red blood cells in 509 patients with microcytosis and erythrocytosis caused by various conditions [2].

High impact information on (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium

• Morphologic observation of the neonatal RBCs established the fact that only puckered RBCs that exhibited brilliant cresyl blue staining reticulum were capable of undergoing receptor-mediated endocytosis of transferrin [5].
• However, significant differences in nuclear-encoded regulatory protein expression were observed between BCB(+) and BCB(-) oocytes and between untreated oocytes and those treated with ddC [6].
• Bone marrow supravital staining with brilliant cresyl blue and electron microscopy studies showed large inclusion bodies in about 3% of erythroblasts [7].
• There was significant correlation of our method with the conventional immunofluorescence staining method for F-cells (r2 = 0.99; slope = 0.99) and with the accepted brilliant cresyl blue method for reticulocytes (r2 = 0.97; slope = 0.96) [8].
• The demonstration of HbH inclusions in red blood cells after incubation with brilliant cresyl blue confirms the diagnosis [9].

Biological context of (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium

• In Exp. 2, embryos were cultured with D-[1-14C]glucose, and L-[3,4-3H(N)]glutamine (a measure of Krebs cycle activity) in the presence of brilliant cresyl blue, a stimulator of the PPP [10].
• More widespread use of brilliant cresyl blue staining for HbH inclusions in individuals with the facial phenotype of ATR-X and/or ambiguous genitalia may lead to the identification of more affected patients and improved understanding of the clinical spectrum of ATR-X [11].
• The following vital tests were found to be useful: succinic dehydrogenase for parietal cells, Nile blue/brilliant cresyl blue stains for chief cells, and different phagocytosis assays for endothelial cells and gastric phagocytes [12].
• In contrast, useful membrane potential indicators were more difficult to find, with Di-4-ANEPPS and Brilliant cresyl blue being the only promising candidates for further studies [13].
• We show here that acidification and recovery converted the insulated interphase nuclei of KB carcinoma and nontumorigenic Chang cells into intense nuclear accumulating states marked by sequestration of the exogenous supravital dyes neutral red, methylene blue, and brilliant cresyl blue [14].

Anatomical context of (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium

• Brilliant cresyl blue staining of blood of these 3 cats revealed prominent Heinz bodies within, and projecting from, the erythrocytes [15].
• Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer [16].
• Brilliant cresyl blue causes the formation of a large number of small inclusions which are invariably attached to the inner surface of the red cell membrane [17].
• It seems likely that the positive lysosomal reactions were obtained with younger blood cells which did not stain with brilliant cresyl blue but still retained single lysosomes in their cytoplasm [18].
• The present study was designed to evaluate the brilliant cresyl blue (BCB) test as a means of selection of immature pig oocytes to improve the performance of the homologous in vitro penetration (hIVP) assay carried out with immature oocytes to assess the penetrating ability of boar spermatozoa [19].

Associations of (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium with other chemical compounds

• Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation [20].
• The author describes age-related changes in the disintegration of rat red blood cells (RBC) caused by brilliant cresyl blue (BCB) in isotonic saline containing NaCl and phosphates [21].
• This paper describes a comparison between the microscopic brilliant cresyl blue and the flow cytometric thiazol orange (FACScan) and auramine O (Sysmex) methods for enumeration of reticulocytes [22].
• Electron microscope studies have been performed to investigate the ultrastructural basis of the characteristic golf-ball-like light microscope appearance which develops when the erythrocytes of patients with HbH disease are stained supravitally with brilliant cresyl blue or new methylene blue [23].
• The staining for Hb H inclusion bodies uses brilliant cresyl blue (BCB) or methylene blue (MB) as an oxidant to denature Hb H as intracellular inclusions [24].

Gene context of (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium

• For this purpose, prior to in vitro maturation (IVM), oocytes were selected for their developmental competence on the basis of glucose-6-phosphate dehydrogenase (G6PDH) activity indicated by brilliant cresyl blue (BCB) staining [16].
• The aim of this study was to evaluate the effectiveness and toxicity of single and double application of the brilliant cresyl blue (BCB) test on the selection of porcine oocytes as an indirect measure of oocyte growth for in vitro fertilization (IVF) and nuclear transfer [25].
• In vitro, oocyte competence can be assessed through the presence of glucose-6-phosphate dehydrogenase (G6PD) as indicated by the dye, brilliant cresyl blue (BCB) [26].

Analytical, diagnostic and therapeutic context of (7-amino-2-methyl-phenoxazin-3-ylidene)-diethyl-azanium

• In contrast, Heinz bodies were not identified on scanning electron microscopy of intact erythrocytes of the remaining 2 cats even though Heinz bodies were found on their blood films stained with brilliant cresyl blue [15].
• Using 8 mice per treatment group, intra-assay precision determined for all of the assays in the study showed coefficients of variation of 12.1-28.4% for the brilliant cresyl blue method, 14.1-30.8% for the selective hemolysis method and 8.5-19.7% for the flow cytometry method [27].
• Huge enhancements of 70-80x, corresponding to net enhancements of >10(4), were observed for brilliant cresyl blue test analyte when Ag-coated tips made from or precoated with low refractive index materials were applied [28].

References