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Gene Review

GNA13  -  guanine nucleotide binding protein (G...

Homo sapiens

Synonyms: G alpha-13, G-protein subunit alpha-13, G13, Guanine nucleotide-binding protein subunit alpha-13, MGC46138
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Disease relevance of GNA13

  • The alpha-subunits of the G-proteins G12 and G13 were expressed with a baculovirus system in insect cells and analysed for acylation [1].
  • These genetic and proliferative effects can be induced by activation of receptors that signal through heterotrimeric GTP-binding proteins (G-proteins) of the Gq family, pertussis toxin-sensitive Gi/Go proteins, Gs, or G12/G13 [2].
  • 2. Normal median nerve SEPs (P20-N30, N20-P30, and P25-N35) were recorded from the cortical surface of a patient (G13) undergoing a cortical excision for relief of focal seizures [3].
  • A series of viral reassortants was prepared between equine rotaviruses H1 (G5), H2 (G3), and L338 (G13) and human rotavirus ST3 (G4) [4].
  • Concomitant regulation of Ca2+ mobilization and G13 expression in human erythroleukemia cells [5].

High impact information on GNA13

  • Recombinant p115 RhoGEF and a fusion protein containing the amino terminus of p115 had specific activity as GTPase activating proteins toward the alpha subunits of the G proteins G12 and G13, but not toward members of the Gs, Gi, or Gq subfamilies of Galpha proteins [6].
  • Here, we show that RGS16 inhibits G alpha 13-mediated, RhoA-dependent reversal of stellation and SRE activation [7].
  • These results elucidate a new mechanism whereby a classical RGS protein regulates G alpha 13-mediated signal transduction independently of the RGS box [7].
  • In conclusion, G2A represents a family of GPCRs expressed in lymphocytes that may link diverse stimuli to cytoskeletal reorganization and transcriptional activation through a pathway involving G alpha 13 and RhoA [8].
  • In membranes of human thyroid gland, we immunologically identified alpha subunits of the G proteins Gs short, Gs long, Gi1, Gi2, Gi3, G(o) (Go2 and another form of Go, presumably Go1), Gq, G11, G12, and G13 [9].

Chemical compound and disease context of GNA13

  • However, other pertussis toxin-insensitive G proteins such as Gq, G12 and G13 were unable to interact with the melatonin receptor [10].

Biological context of GNA13


Anatomical context of GNA13

  • Using subtype-specific antisera, we were able to identify the recently described alpha subunits of G12 and G13 in platelet membranes as 43-kDa proteins [12].
  • Treatment of K562 cells with DMSO reduced the levels of thrombin receptor mRNA, without simultaneous changes in the expression of G12 alpha and G13 alpha [15].
  • Consistent with this observation, ADP-ribosylation experiments revealed the presence of two PT substrates which co-migrated with human erythrocyte G12 alpha and G13 alpha [15].
  • Characterization of G alpha 13-dependent plasma membrane recruitment of p115RhoGEF [16].
  • Studies with the constitutively activated mutants of G alpha 12 and G alpha 13 have indicated that they stimulate mitogenic signaling pathways leading to the oncogenic transformation of fibroblast cell lines [17].

Associations of GNA13 with chemical compounds

  • Effective activation of G12 and G13 via the thromboxane A2 and the thrombin receptors was not dependent on the presence of GDP [12].
  • GTP gamma S significantly (P < .05) decreased the affinity of the receptor for 125I-BOP in COS-7 cell membranes coexpressing HEL-TXR and G alpha 13 to a value comparable to HEL-TXA2 receptor alone [18].
  • In separate experiments, it was found that the TXA2 receptor agonist U46619 stimulated [35S]guanosine 5'-O-(3-thiotriphosphate) incorporation into G13 alpha-subunit [19].
  • We were interested especially in the potential of the isoprostane 8-iso-prostaglandin F (8-iso-PGF2alpha), among other ligands examined, to activate G12 and G13 through TPalpha explicitly [20].
  • Fatty acid analysis after labeling with [3H]palmitic acid showed that palmitate represents the predominant fatty acid linked to G alpha 12 and G alpha 13 [1].

Physical interactions of GNA13

  • Using in vitro binding and coimmunoprecipitation assays, we have shown that only activated G alpha 13 binds to AKAP110, suggesting a potential role for AKAP110 as a G alpha subunit effector protein [21].

Regulatory relationships of GNA13

  • G alpha 12 required a higher Mg2+ concentration for AlF4- -induced dissociation from immobilized G beta gamma than did G alpha 13 [22].
  • Importantly, truncated G alpha 13 retained its ability to stimulate apoptosis signal-regulated kinase ASK1 and c-Jun terminal kinase, JNK [23].

Other interactions of GNA13


Analytical, diagnostic and therapeutic context of GNA13


  1. The alpha-subunits of G-proteins G12 and G13 are palmitoylated, but not amidically myristoylated. Veit, M., Nürnberg, B., Spicher, K., Harteneck, C., Ponimaskin, E., Schultz, G., Schmidt, M.F. FEBS Lett. (1994) [Pubmed]
  2. G protein-coupled receptors and signaling pathways regulating growth responses. Post, G.R., Brown, J.H. FASEB J. (1996) [Pubmed]
  3. Cortical somatosensory evoked potentials. II. Effects of excision of somatosensory or motor cortex in humans and monkeys. Allison, T., Wood, C.C., McCarthy, G., Spencer, D.D. J. Neurophysiol. (1991) [Pubmed]
  4. Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes. Isa, P., Snodgrass, D.R. Virology (1994) [Pubmed]
  5. Concomitant regulation of Ca2+ mobilization and G13 expression in human erythroleukemia cells. Michel, M.C. Eur. J. Pharmacol. (1998) [Pubmed]
  6. p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13. Kozasa, T., Jiang, X., Hart, M.J., Sternweis, P.M., Singer, W.D., Gilman, A.G., Bollag, G., Sternweis, P.C. Science (1998) [Pubmed]
  7. RGS16 inhibits signalling through the G alpha 13-Rho axis. Johnson, E.N., Seasholtz, T.M., Waheed, A.A., Kreutz, B., Suzuki, N., Kozasa, T., Jones, T.L., Brown, J.H., Druey, K.M. Nat. Cell Biol. (2003) [Pubmed]
  8. Direct genetic demonstration of G alpha 13 coupling to the orphan G protein-coupled receptor G2A leading to RhoA-dependent actin rearrangement. Kabarowski, J.H., Feramisco, J.D., Le, L.Q., Gu, J.L., Luoh, S.W., Simon, M.I., Witte, O.N. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  9. The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families. Laugwitz, K.L., Allgeier, A., Offermanns, S., Spicher, K., Van Sande, J., Dumont, J.E., Schultz, G. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  10. Stimulation of cAMP accumulation by the cloned Xenopus melatonin receptor through Gi and Gz proteins. Yung, L.Y., Tsim, S.T., Wong, Y.H. FEBS Lett. (1995) [Pubmed]
  11. Expression of GTP-binding protein alpha subunits in human thymocytes. Kabouridis, P.S., Waters, S.T., Escobar, S., Stanners, J., Tsoukas, C.D. Mol. Cell. Biochem. (1995) [Pubmed]
  12. G proteins of the G12 family are activated via thromboxane A2 and thrombin receptors in human platelets. Offermanns, S., Laugwitz, K.L., Spicher, K., Schultz, G. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  13. Galpha12 and galpha13 are phosphorylated during platelet activation. Offermanns, S., Hu, Y.H., Simon, M.I. J. Biol. Chem. (1996) [Pubmed]
  14. Coupling interaction between thromboxane A2 receptor and alpha-13 subunit of guanine nucleotide-binding protein. Chou, K.C. J. Proteome Res. (2005) [Pubmed]
  15. Ca2+ signalling in K562 human erythroleukaemia cells: effect of dimethyl sulphoxide and role of G-proteins in thrombin- and thromboxane A2-activated pathways. Thomas, C.P., Dunn, M.J., Mattera, R. Biochem. J. (1995) [Pubmed]
  16. Characterization of G alpha 13-dependent plasma membrane recruitment of p115RhoGEF. Bhattacharyya, R., Wedegaertner, P.B. Biochem. J. (2003) [Pubmed]
  17. Signaling by the G12 class of G proteins. Dhanasekaran, N., Dermott, J.M. Cell. Signal. (1996) [Pubmed]
  18. Characterization of the cloned HEL cell thromboxane A2 receptor: evidence that the affinity state can be altered by G alpha 13 and G alpha q. Allan, C.J., Higashiura, K., Martin, M., Morinelli, T.A., Kurtz, D.T., Geoffroy, O., Meier, G.P., Gettys, T.W., Halushka, P.V. J. Pharmacol. Exp. Ther. (1996) [Pubmed]
  19. Identification of Galpha13 as one of the G-proteins that couple to human platelet thromboxane A2 receptors. Djellas, Y., Manganello, J.M., Antonakis, K., Le Breton, G.C. J. Biol. Chem. (1999) [Pubmed]
  20. The G12 family of G proteins as a reporter of thromboxane A2 receptor activity. Zhang, L., DiLizio, C., Kim, D., Smyth, E.M., Manning, D.R. Mol. Pharmacol. (2006) [Pubmed]
  21. Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation. Niu, J., Vaiskunaite, R., Suzuki, N., Kozasa, T., Carr, D.W., Dulin, N., Voyno-Yasenetskaya, T.A. Curr. Biol. (2001) [Pubmed]
  22. Distinct biochemical properties of the native members of the G12 G-protein subfamily. Characterization of G alpha 12 purified from rat brain. Harhammer, R., Nürnberg, B., Harteneck, C., Leopoldt, D., Exner, T., Schultz, G. Biochem. J. (1996) [Pubmed]
  23. G alpha 13-mediated transformation and apoptosis are permissively dependent on basal ERK activity. Adarichev, V.A., Vaiskunaite, R., Niu, J., Balyasnikova, I.V., Voyno-Yasenetskaya, T.A. Am. J. Physiol., Cell Physiol. (2003) [Pubmed]
  24. Functional consequences of G alpha 13 mutations that disrupt interaction with p115RhoGEF. Grabocka, E., Wedegaertner, P.B. Oncogene (2005) [Pubmed]
  25. Regulation of apoptosis by alpha-subunits of G12 and G13 proteins via apoptosis signal-regulating kinase-1. Berestetskaya, Y.V., Faure, M.P., Ichijo, H., Voyno-Yasenetskaya, T.A. J. Biol. Chem. (1998) [Pubmed]
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