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Gene Review

YWHAQ  -  tyrosine 3-monooxygenase/tryptophan 5...

Homo sapiens

Synonyms: 14-3-3, 14-3-3 protein T-cell, 14-3-3 protein tau, 14-3-3 protein theta, 1C5, ...
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Disease relevance of YWHAQ


High impact information on YWHAQ


Biological context of YWHAQ


Anatomical context of YWHAQ

  • Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling [9].
  • Expression of the human HS1 protein from an expression vector introduced into one of the variant cell lines restored the sensitivity of the cells to apoptosis induced by surface IgM crosslinking [5].
  • Additionally, no tyrosine phosphorylation of HS1 was induced by mitogenic pairs of anti-CD2 mAbs capable of activating the transcription factor NFAT (nuclear factor of activated T cells) [8].
  • All together these results indicate that HS1 Tyr phosphorylation catalyzed by Syk and Lyn plays a crucial role in the translocation of the protein to the membrane and is involved in the cytoskeleton rearrangement triggered by thrombin in human platelets [10].
  • Immunofluorescence microscopic analysis shows that the agonist promotes HS1 migration to the plasma membrane and that the inhibition of Lyn-mediated secondary phosphorylation of HS1 abrogates the subcellular translocation of the protein [10].

Associations of YWHAQ with chemical compounds

  • 1C5 recognizes the region 119-130 corresponding to the GXXXG motif, the glycine zipper region, conserved in all mammals [11].
  • By means of specific inhibitors (PP2, SU6656, and piceatannol) and phosphopeptide-specific antibodies, as well as by coimmunoprecipitation and binding competition experiments, we show that Syk acts as the primary kinase that phosphorylates HS1 at Tyr397 and that Syk phosphorylation is required for HS1 interaction with the Lyn SH2 domain [10].
  • 1C5 can be used for the staining of routine formalin-fixed and paraffin-embedded tissue sections [3].
  • 1C5 also reacts with 95% ethanol-fixed malignant cells in cervical smears [3].
  • Related studies have shown that citrate removed calcium bound by PPG; N6-substituted adenines were bound by PPG while three polyphosphorylated dinucleosides, HS3, HS2, and HS1, were displaced from it [12].

Other interactions of YWHAQ


Analytical, diagnostic and therapeutic context of YWHAQ


  1. A 14-3-3 mRNA is up-regulated in amyotrophic lateral sclerosis spinal cord. Malaspina, A., Kaushik, N., de Belleroche, J. J. Neurochem. (2000) [Pubmed]
  2. HS1 protein is differentially expressed in chronic lymphocytic leukemia patient subsets with good or poor prognoses. Scielzo, C., Ghia, P., Conti, A., Bachi, A., Guida, G., Geuna, M., Alessio, M., Caligaris-Cappio, F. J. Clin. Invest. (2005) [Pubmed]
  3. New monoclonal antibody, 1C5, reactive with human cervical adenocarcinoma of the uterus, with immunodiagnostic potential. Koizumi, M., Uede, T., Shijubo, N., Kudo, R., Hashimoto, M., Kikuchi, K. Cancer Res. (1988) [Pubmed]
  4. Detection of macrophage migration inhibitory factor by monoclonal antibody in Sézary syndrome. Neumann, C., Schlegel, R., Steckel, F., Sorg, C. J. Invest. Dermatol. (1987) [Pubmed]
  5. Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate. Fukuda, T., Kitamura, D., Taniuchi, I., Maekawa, Y., Benhamou, L.E., Sarthou, P., Watanabe, T. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  6. Characterization of the DNase I hypersensitive site 3' of the human beta globin gene domain. Fleenor, D.E., Kaufman, R.E. Blood (1993) [Pubmed]
  7. Molecular features underlying the sequential phosphorylation of HS1 protein and its association with c-Fgr protein-tyrosine kinase. Brunati, A.M., Donella-Deana, A., James, P., Quadroni, M., Contri, A., Marin, O., Pinna, L.A. J. Biol. Chem. (1999) [Pubmed]
  8. Uncoupling activation-dependent HS1 phosphorylation from nuclear factor of activated T cells transcriptional activation in Jurkat T cells: differential signaling through CD3 and the costimulatory receptors CD2 and CD28. Hutchcroft, J.E., Slavik, J.M., Lin, H., Watanabe, T., Bierer, B.E. J. Immunol. (1998) [Pubmed]
  9. Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling. Yamanashi, Y., Okada, M., Semba, T., Yamori, T., Umemori, H., Tsunasawa, S., Toyoshima, K., Kitamura, D., Watanabe, T., Yamamoto, T. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  10. Thrombin-induced tyrosine phosphorylation of HS1 in human platelets is sequentially catalyzed by Syk and Lyn tyrosine kinases and associated with the cellular migration of the protein. Brunati, A.M., Deana, R., Folda, A., Massimino, M.L., Marin, O., Ledro, S., Pinna, L.A., Donella-Deana, A. J. Biol. Chem. (2005) [Pubmed]
  11. Generation of Monoclonal Antibody Recognized by the GXXXG Motif (Glycine Zipper) of Prion Protein. Choi, J.K., Park, S.J., Jun, Y.C., Oh, J.M., Jeong, B.H., Lee, H.P., Park, S.N., Carp, R.I., Kim, Y.S. Hybridoma (2005) (2006) [Pubmed]
  12. Glucose transport in Achlya: characterization and possible regulatory aspects. Goh, S.H., LéJohn, H.B. Can. J. Biochem. (1978) [Pubmed]
  13. 14-3-3 protein masks the DNA binding interface of forkhead transcription factor FOXO4. Silhan, J., Vacha, P., Strnadova, P., Vecer, J., Herman, P., Sulc, M., Teisinger, J., Obsilova, V., Obsil, T. J. Biol. Chem. (2009) [Pubmed]
  14. Human factors impact successful lay person automated external defibrillator use during simulated cardiac arrest. Callejas, S., Barry, A., Demertsidis, E., Jorgenson, D., Becker, L.B. Crit. Care Med. (2004) [Pubmed]
  15. Purification and characterization of Heteroscorpine-1 (HS-1) toxin from Heterometrus laoticus scorpion venom. Uawonggul, N., Thammasirirak, S., Chaveerach, A., Arkaravichien, T., Bunyatratchata, W., Ruangjirachuporn, W., Jearranaiprepame, P., Nakamura, T., Matsuda, M., Kobayashi, M., Hattori, S., Daduang, S. Toxicon (2007) [Pubmed]
  16. Immunohistochemical comparison of new monoclonal antibody 1C5 and carcinoembryonic antigen in the differential diagnosis of adenocarcinoma of the uterine cervix. Kudo, R., Sasano, H., Koizumi, M., Orenstein, J.M., Silverberg, S.G. Int. J. Gynecol. Pathol. (1990) [Pubmed]
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