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Akap5  -  A kinase (PRKA) anchor protein 5

Rattus norvegicus

Synonyms: A-kinase anchor protein 150 kDa, A-kinase anchor protein 5, AKAP 150, AKAP-5, AKAP150, ...
 
 
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Psychiatry related information on Akap5

 

High impact information on Akap5

  • Here we show that the regulatory subunits of PKA are colocalized with AKAP150 (an AKAP isoform that is expressed in the brain) in the lateral amygdala (LA) and that infusion to the LA of the peptide St-Ht31, which blocks PKA anchoring onto AKAPs, impairs memory consolidation of auditory fear conditioning [2].
  • The protocol involves a sequence of: (a) ammonium sulfate precipitation, (b) DEAE-cellulose ion exchange chromatography, (c) gel filtration chromatography on Bio-Gel P150 in 1.0 M ammonium acetate, (d) hydrophobic chromatography on dodecyl agarose, and (e) gel filtration chromatography in 6.0 M guanidine hydrochloride [3].
  • AKAP 150 is abundant in Purkinje cells and in neurons of the olfactory bulb, basal ganglia, cerebral cortex, and other forebrain regions [4].
  • cAMP signaling in neurons: patterns of neuronal expression and intracellular localization for a novel protein, AKAP 150, that anchors the regulatory subunit of cAMP-dependent protein kinase II beta [4].
  • A high proportion of total AKAP 150 is concentrated in primary branches of dendrites, where it is associated with microtubules [4].
 

Biological context of Akap5

  • High affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II-B. Cloning, characterization, and expression of cDNAs for rat brain P150 [5].
  • LTP but not seizure is associated with up-regulation of AKAP-150 [1].
  • Expression of AKAP-150 and quick down-regulation of Na(+),K(+)-ATPase by AKAP-anchored PKA in response to cAMP elevation are characteristics specific to PG among the three major salivary glands, suggesting the presence of PG-specific regulatory mechanisms for saliva production/secretion [6].
  • Thus, the mechanisms behind exercise training-enhanced adipocyte lipolysis could involve the increased activities of PKA and HSL with enhanced expressions of AKAP150 and some subunits of PKA, all of which may be compartmentalized within adipocytes [7].
 

Anatomical context of Akap5

 

Associations of Akap5 with chemical compounds

  • In addition, polyproteins encoded by both of these FeSV isolates bound to, and phosphorylated tyrosine acceptor sites within, a 150,000-molecular-weight cellular substrate (P150) [10].
  • Moreover, total cellular levels of phosphotyrosine remained unaltered in McDonough FeSV-transformed cells, and the major McDonough FeSV polyprotein translational product lacked binding affinity for P150 [10].
  • There was some overlap between AKAP150 and GABA receptor type A receptor beta2/3-immunoreactivity intracellularly in perinuclear clusters [11].
  • This implies that the AKAP-150 harbours a novel property of selective responsiveness to the stimulation patterns that trigger NMDA-dependent LTP in vivo [1].
  • Its selective up-regulation during LTP and its identified functions as a scaffold for protein kinase A, protein kinase C, calmodulin, calcineurin and ionotropic glutamate receptors suggest that AKAP-150 encodes is an important effector protein in the expression of late LTP [1].
 

Regulatory relationships of Akap5

 

Analytical, diagnostic and therapeutic context of Akap5

  • Sequence analyses disclosed that P150 is a previously uncharacterized protein that contains multiple octapeptide repeats as well as unique sequences [5].
  • AKAP79 also associates with conventional, novel and atypical isoforms of PKC in vitro and in vivo, and immunofluorescence staining of rat hippocampal neurons demonstrates that the murine anchoring-protein homologue AKAP150 is co-distributed with PKCalpha/beta, PKCepsilon or PKCiota [12].
  • AKAP-150 protein in BLMVs was shown by immunoblotting and an RII overlay assay and was coimmunoprecipitated by anti-RII antibody [13].
  • We have combined this with in situ hybridization and have identified A-kinase anchoring protein of 150 kDa (AKAP-150) as a gene selectively up-regulated during the maintenance phase of LTP [1].
  • The localization of neurofilament triplet proteins in PC12 cells grown in the absence of (PC12-) or maintained in the presence of (PC12+) nerve growth factor (NGF) was studied using indirect immunofluorescence and monospecific, immunosorbent purified antibodies to 68,000 (P68), 150,000 (P150) and 200,000 (P200) dalton neurofilament proteins [14].

References

  1. LTP but not seizure is associated with up-regulation of AKAP-150. Génin, A., French, P., Doyère, V., Davis, S., Errington, M.L., Maroun, M., Stean, T., Truchet, B., Webber, M., Wills, T., Richter-Levin, G., Sanger, G., Hunt, S.P., Mallet, J., Laroche, S., Bliss, T.V., O'Connor, V. Eur. J. Neurosci. (2003) [Pubmed]
  2. A-kinase anchoring proteins in amygdala are involved in auditory fear memory. Moita, M.A., Lamprecht, R., Nader, K., LeDoux, J.E. Nat. Neurosci. (2002) [Pubmed]
  3. Mitogenic polypeptide of the mammalian seminiferous epithelium: biochemical characterization and partial purification. Feig, L.A., Klagsbrun, M., Bellvé, A.R. J. Cell Biol. (1983) [Pubmed]
  4. cAMP signaling in neurons: patterns of neuronal expression and intracellular localization for a novel protein, AKAP 150, that anchors the regulatory subunit of cAMP-dependent protein kinase II beta. Glantz, S.B., Amat, J.A., Rubin, C.S. Mol. Biol. Cell (1992) [Pubmed]
  5. High affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II-B. Cloning, characterization, and expression of cDNAs for rat brain P150. Bregman, D.B., Bhattacharyya, N., Rubin, C.S. J. Biol. Chem. (1989) [Pubmed]
  6. Specific expression of an A-kinase anchoring protein subtype, AKAP-150, and specific regulatory mechanism for Na(+),K(+)-ATPase via protein kinase A in the parotid gland among the three major salivary glands of the rat. Kurihara, K., Nakanishi, N., Amano, O., Yamamoto, M., Iseki, S. Biochem. Pharmacol. (2003) [Pubmed]
  7. Possible mechanisms by which adipocyte lipolysis is enhanced in exercise-trained rats. Nomura, S., Kawanami, H., Ueda, H., Kizaki, T., Ohno, H., Izawa, T. Biochem. Biophys. Res. Commun. (2002) [Pubmed]
  8. Regulation of Na,K-ATPase by cAMP-dependent protein kinase anchored on membrane via A-kinase anchoring protein subtype, AKAP-150, in rat parotid gland. Kurihara, K., Nakanishi, N. Ann. N. Y. Acad. Sci. (2003) [Pubmed]
  9. Progesterone prevents the pregnancy-related decline in protein kinase A association with rat myometrial plasma membrane and A-kinase anchoring protein. Ku, C.Y., Sanborn, B.M. Biol. Reprod. (2002) [Pubmed]
  10. Differences in mechanisms of transformation by independent feline sarcoma virus isolates. Reynolds, F.H., Van de Ven, W.J., Blomberg, J., Stephenson, J.R. J. Virol. (1981) [Pubmed]
  11. Synaptic and subcellular localization of A-kinase anchoring protein 150 in rat hippocampal CA1 pyramidal cells: Co-localization with excitatory synaptic markers. Lilly, S.M., Alvarez, F.J., Tietz, E.I. Neuroscience (2005) [Pubmed]
  12. Mechanism of A-kinase-anchoring protein 79 (AKAP79) and protein kinase C interaction. Faux, M.C., Rollins, E.N., Edwards, A.S., Langeberg, L.K., Newton, A.C., Scott, J.D. Biochem. J. (1999) [Pubmed]
  13. Regulation of Na(+)-K(+)-ATPase by cAMP-dependent protein kinase anchored on membrane via its anchoring protein. Kurihara, K., Nakanishi, N., Ueha, T. Am. J. Physiol., Cell Physiol. (2000) [Pubmed]
  14. Induction of neurofilament triplet proteins in PC12 cells by nerve growth factor. Lee, V., Trojanowski, J.Q., Schlaepfer, W.W. Brain Res. (1982) [Pubmed]
 
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