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Gene Review

Pou2af1  -  POU domain, class 2, associating factor 1

Mus musculus

Synonyms: B-cell-specific coactivator OBF-1, BOB-1, BOB.1, BOB1, Bob-1, ...
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Disease relevance of Pou2af1


High impact information on Pou2af1

  • The later identification of a membrane bound, myristoylated form of OCA-B suggested additional, unique functions in B cell signaling pathways [4].
  • This study has shown that OCA-B also functions in the pre-B1-to-pre-B2 cell transition and, most surprisingly, that it directly interacts with SYK, a tyrosine kinase critical for pre-BCR and BCR signaling [4].
  • OcaB, a transcriptional coactivator also known as Bob-1 or OBF-1, was isolated on the basis of its ability to enhance transcription of immunoglobulin (Ig) genes in vitro [5].
  • To define the in vivo role of OBF-1 we have used gene targeting in embryonic stem cells to generate mice lacking the coactivator OBF-1 [6].
  • Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells [7].

Biological context of Pou2af1


Anatomical context of Pou2af1

  • Taken together, these results suggest that, at least in conventional B cells, Bob1 plays in important role in the antigen-driven stages of B cell activation and maturation [10].
  • Histological examination of sections from spleen and lymph nodes reveal that while Bob-1-deficient mice have primary follicles, they lack well-developed germinal centers [10].
  • RNAs differentially expressed in a BOB.1/OBF.1-deficient pre-B cell line and a derivative of this cell line expressing a hormone dependent BOB.1/OBF.1-estrogen receptor (BobER) fusion protein were isolated [8].
  • BOB.1/OBF.1 can efficiently activate octamer-dependent promoters in fibroblasts; however, it fails to stimulate octamer-dependent enhancer activity [11].
  • When BOB.1/OBF.1-deficient mice were crossed with Bcl2-transgenic mice, B cell development in the bone marrow and numbers of B cells in peripheral lymphoid organs were normalized [12].

Associations of Pou2af1 with chemical compounds

  • Induction of pre-B cells with lipopolysaccharide led to increased Oct2 levels but did not significantly increase octamer-dependent transcription in BOB.1 / OBF.1-deficient B cells [13].
  • In search of BOB.1/OBF.1 regulated genes we identified Btk--a cytoplasmic tyrosine kinase-as a direct target of BOB.1/OBF [14].
  • We show that BOB.1/OBF.1(-/-) B cells were incapable to induce BCR-triggered Ca(2+) mobilization [15].
  • Intracellular calcium (Ca2+i) was mobilized in the control cell lines but not in the OBF-1 and Vav1-deficient cells, while Xid B cell lines (btk mutant) showed a reduced Ca2+ mobilization [16].

Physical interactions of Pou2af1

  • These results indicate that Oct-2 and OCA-B interact with the 3' enhancer in regulation of the IgH locus during B cell activation [17].

Regulatory relationships of Pou2af1

  • Myosin light chain 1 atrial isoform (MLC1A) is expressed in pre-B cells under control of the BOB.1/OBF.1 coactivator [8].
  • These findings support the notion that Oct-2 regulates gene transcription by both OBF-1-dependent and -independent mechanisms [18].
  • Under these conditions, the loss of OBF-1 blocks the genetic program of ASC differentiation so that Blimp-1/prdm1 induction fails, and bcl-6, Pax5, and AID are not repressed as in control ASC [19].

Other interactions of Pou2af1

  • Consistent with this conclusion is the observation that transcriptional induction of the endogenous MLC1A gene by BOB.1/OBF.1 requires de novo protein synthesis [8].
  • Probably as a consequence of this reduction in mature B cells, Bob1-deficient mice show reduced serum titers of the immunoglobulin isotypes IgG1, IgG2a, IgG2b and IgA, but not IgM [10].
  • These results suggest that NFKB1 and OCA-B play compensatory roles in multiple aspects of B cell differentiation [20].
  • Oct-2 regulates CD36 gene expression via a consensus octamer, which excludes the co-activator OBF-1 [18].
  • 1. Btk, like BOB.1/OBF.1, plays a critical role in B cell development and B cell receptor signalling [14].

Analytical, diagnostic and therapeutic context of Pou2af1


  1. Up-regulation of BOB.1/OBF.1 expression in normal germinal center B cells and germinal center-derived lymphomas. Greiner, A., Müller, K.B., Hess, J., Pfeffer, K., Müller-Hermelink, H.K., Wirth, T. Am. J. Pathol. (2000) [Pubmed]
  2. Cutting edge: lack of peripheral B cells and severe agammaglobulinemia in mice simultaneously lacking Bruton's tyrosine kinase and the B cell-specific transcriptional coactivator OBF-1. Schubart, D.B., Rolink, A., Schubart, K., Matthias, P. J. Immunol. (2000) [Pubmed]
  3. Oct-2 and Bob-1 deficiency in Hodgkin and Reed Sternberg cells. Re, D., Müschen, M., Ahmadi, T., Wickenhauser, C., Staratschek-Jox, A., Holtick, U., Diehl, V., Wolf, J. Cancer Res. (2001) [Pubmed]
  4. Nontranscriptional regulation of SYK by the coactivator OCA-B is required at multiple stages of B cell development. Siegel, R., Kim, U., Patke, A., Yu, X., Ren, X., Tarakhovsky, A., Roeder, R.G. Cell (2006) [Pubmed]
  5. OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes. Casellas, R., Jankovic, M., Meyer, G., Gazumyan, A., Luo, Y., Roeder, R., Nussenzweig, M. Cell (2002) [Pubmed]
  6. B-cell-specific coactivator OBF-1/OCA-B/Bob1 required for immune response and germinal centre formation. Schubart, D.B., Rolink, A., Kosco-Vilbois, M.H., Botteri, F., Matthias, P. Nature (1996) [Pubmed]
  7. Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells. Zwilling, S., Dieckmann, A., Pfisterer, P., Angel, P., Wirth, T. Science (1997) [Pubmed]
  8. Myosin light chain 1 atrial isoform (MLC1A) is expressed in pre-B cells under control of the BOB.1/OBF.1 coactivator. Laumen, H., Brunner, C., Greiner, A., Wirth, T. Nucleic Acids Res. (2004) [Pubmed]
  9. The B lymphocyte-specific coactivator BOB.1/OBF.1 is required at multiple stages of B-cell development. Hess, J., Nielsen, P.J., Fischer, K.D., Bujard, H., Wirth, T. Mol. Cell. Biol. (2001) [Pubmed]
  10. B lymphocytes are impaired in mice lacking the transcriptional co-activator Bob1/OCA-B/OBF1. Nielsen, P.J., Georgiev, O., Lorenz, B., Schaffner, W. Eur. J. Immunol. (1996) [Pubmed]
  11. Functional characterization of the murine homolog of the B cell-specific coactivator BOB.1/OBF.1. Pfisterer, P., Zwilling, S., Hess, J., Wirth, T. J. Biol. Chem. (1995) [Pubmed]
  12. B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice. Brunner, C., Marinkovic, D., Klein, J., Samardzic, T., Nitschke, L., Wirth, T. J. Exp. Med. (2003) [Pubmed]
  13. The BOB.1 / OBF.1 co-activator is essential for octamer-dependent transcription in B cells. Laumen, H., Nielsen, P.J., Wirth, T. Eur. J. Immunol. (2000) [Pubmed]
  14. Btk expression is controlled by Oct and BOB.1/OBF.1. Brunner, C., Wirth, T. Nucleic Acids Res. (2006) [Pubmed]
  15. CD22 regulates early B cell development in BOB.1/OBF.1-deficient mice. Samardzic, T., Gerlach, J., Muller, K., Marinkovic, D., Hess, J., Nitschke, L., Wirth, T. Eur. J. Immunol. (2002) [Pubmed]
  16. Knockout B lymphoma cell lines as biochemical tools to explore multiple signalling pathways. Veale, M.F., Dietrich, W.M., Corcoran, L.M. Immunol. Cell Biol. (2003) [Pubmed]
  17. Transcriptional regulation of the murine 3' IgH enhancer by OCT-2. Tang, H., Sharp, P.A. Immunity (1999) [Pubmed]
  18. Oct-2 regulates CD36 gene expression via a consensus octamer, which excludes the co-activator OBF-1. Shore, P., Dietrich, W., Corcoran, L.M. Nucleic Acids Res. (2002) [Pubmed]
  19. Differential requirement for OBF-1 during antibody-secreting cell differentiation. Corcoran, L.M., Hasbold, J., Dietrich, W., Hawkins, E., Kallies, A., Nutt, S.L., Tarlinton, D.M., Matthias, P., Hodgkin, P.D. J. Exp. Med. (2005) [Pubmed]
  20. Genetic analyses of NFKB1 and OCA-B function: defects in B cells, serum IgM level, and antibody responses in Nfkb1-/-Oca-b-/- mice. Kim, U., Gunther, C.S., Roeder, R.G. J. Immunol. (2000) [Pubmed]
  21. Expression of the aldehyde dehydrogenase 2-like gene is controlled by BOB.1/OBF.1 in B lymphocytes. Brunner, C., Laumen, H., Nielsen, P.J., Kraut, N., Wirth, T. J. Biol. Chem. (2003) [Pubmed]
  22. The B-cell-specific transcription coactivator OCA-B/OBF-1/Bob-1 is essential for normal production of immunoglobulin isotypes. Kim, U., Qin, X.F., Gong, S., Stevens, S., Luo, Y., Nussenzweig, M., Roeder, R.G. Nature (1996) [Pubmed]
  23. Identification of transcription coactivator OCA-B-dependent genes involved in antigen-dependent B cell differentiation by cDNA array analyses. Kim, U., Siegel, R., Ren, X., Gunther, C.S., Gaasterland, T., Roeder, R.G. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  24. The Ets factor Spi-B is a direct critical target of the coactivator OBF-1. Bartholdy, B., Du Roure, C., Bordon, A., Emslie, D., Corcoran, L.M., Matthias, P. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
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