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Gene Review:

Ccl12 - chemokine (C-C motif) ligand 12

Mus musculus

Synonyms: C-C motif chemokine 12, MCP-1-related chemokine, MCP-5, Mcp5, Monocyte chemoattractant protein 5, ...

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Disease relevance of Ccl12

The role of CCL12 in the recruitment of fibrocytes and lung fibrosis [1].
From these results, we postulate that mMCP-5 plays a pivotal role during the early stages of allergic lung inflammation [2].
We tested the inhibitory properties of S. epidermidis strains RP62A (slime producer) and RP62NA (nonslime producer) and Staphylococcus aureus strain 502a, using an in vitro tritiated thymidine murine fibroblast (ATCC CCL-12) proliferation assay [3].

High impact information on Ccl12

MCP-5 induced a calcium flux in peripheral blood mononuclear cells, but not in purified murine eosinophils or neutrophils [4].
These data indicate that MCP-5 is a novel and potent monocyte active chemokine that is involved in allergic inflammation and the host response to pathogens [4].
Constitutive expression of MCP-5 mRNA was detected predominantly in lymph nodes, and its expression was markedly induced in macrophages activated in vitro and in vivo [4].
In contrast to Eotaxin, mRNA for mMCP-5 can be produced by mast cells [2].
The presence of lymphocytes is essential for expression of mMCP-5 by alveolar macrophages and smooth muscle cells in the lung, and the induction of mMCP-5 RNA occurs earlier than that of the eosinophil chemokine Eotaxin during allergic inflammation [2].

Biological context of Ccl12

CCL2 and CCL12 are both elevated in the lung after FITC injury, but with different kinetics [1].
0. Microsequence analyses showed that two polypeptides of about 29 and 30 kDa had NH2 terminal amino acid sequences homologous to mouse MC proteinase 5 (MCP-5), whereas the amino terminals of the 33, 35, and 36 kDa proteins were homologous to MC carboxypeptidase A (MC-CPA) [5].
Based on the expression on various mast cell-specific proteases and several additional hematopoietic differentiation markers, the cell lines CFTL-15 and MCP5 / L were clearly identified as mast cells, although with a relatively immature phenotype [6].
Murine mast cells of line MCP5/L were used to investigate the effect of antigenic stimulation on cell adhesion to fN and mediator secretion. effect of antigenic stimulation on cell adhesion to FN and mediator secretion [7].

Anatomical context of Ccl12

Neutralization of mMCP-5 protein with specific antibodies during an allergic inflammatory reaction in vivo resulted in a reduction in the number of eosinophils that accumulated in the lung [2].
Under noninflammatory conditions, expression of mMCP-5 in the lymph nodes and thymus is constitutive and is generally restricted to stromal cells [2].
Immunohistochemistry for MCP-1, MCP-5, and MIP-1alpha located their expression to plaque macrophages [8].
We thus first identified MC-1 on bone marrow cultured murine mast cells (BMCMC) and a murine mast cell line (MCP-5) employing flow cytometry and through detection of specific binding [9].
The nontreated, heat-treated, or trypsin-digested in vivo PGF was incubated with an in vitro [3H]thymidine murine fibroblast (ATCC CCL-12) proliferation assay [10].

Associations of Ccl12 with chemical compounds

However, addition of recombinant IFN-gamma resulted in markedly increased secretion of MCP-1 and MCP-5 only in the cells derived from estrogen-treated mice [11].
RT-PCR and cytofluorimetric analysis were used to assess the expression of alpha 5 integrin in MCP5/L mast cells [12].

Other interactions of Ccl12

A kinetic analysis showed that chemokines tended to increase with stimulation time, but only MCP-1 and MCP-5 showed a biological trend of increasing in splenocytes from estrogen-treated mice, and KC was decreased significantly in estrogen-treated splenocytes at 18 h [11].
ELISA for MCP-1 and MCP-5 showed similar results [8].
Real-time polymerase chain reaction revealed a plaque progression-dependent increase in mRNA levels of MCP-1, MCP-5, MIP-1alpha, and MIP-1beta [8].
An inhibiting antibody for MCP-1 and MCP-5 (11K2) was designed and administered to ApoE-/- mice for 12 weeks starting at the age of 5 or 17 weeks [8].
Murine bone marrow-cultured mast cells (BMCMC) and peritoneal mast cells, as well as two mast cell lines (C57 and MCP-5), constitutively expressed Fas Ag [13].

References

The role of CCL12 in the recruitment of fibrocytes and lung fibrosis. Moore, B.B., Murray, L., Das, A., Wilke, C.A., Herrygers, A.B., Toews, G.B. Am. J. Respir. Cell Mol. Biol. (2006) [Pubmed]
Distinct expression and function of the novel mouse chemokine monocyte chemotactic protein-5 in lung allergic inflammation. Jia, G.Q., Gonzalo, J.A., Lloyd, C., Kremer, L., Lu, L., Martinez-A, C., Wershil, B.K., Gutierrez-Ramos, J.C. J. Exp. Med. (1996) [Pubmed]
Bacterial components inhibit fibroblast proliferation in vitro. Edds, E.M., Bergamini, T.M., Brittian, K.R. ASAIO journal (American Society for Artificial Internal Organs : 1992) (2000) [Pubmed]
Murine monocyte chemoattractant protein (MCP)-5: a novel CC chemokine that is a structural and functional homologue of human MCP-1. Sarafi, M.N., Garcia-Zepeda, E.A., MacLean, J.A., Charo, I.F., Luster, A.D. J. Exp. Med. (1997) [Pubmed]
Proteinases of rat mast cells. Peritoneal but not intestinal mucosal mast cells express mast cell proteinase 5 and carboxypeptidase A. Befus, A.D., Chin, B., Pick, J., Evans, S., Osborn, S., Forstrom, J. J. Immunol. (1995) [Pubmed]
Murine mast cell lines as indicators of early events in mast cell and basophil development. Lunderius, C., Xiang, Z., Nilsson, G., Hellman, L. Eur. J. Immunol. (2000) [Pubmed]
Relations between Fc epsilon RI crosslinking-induced mast cell activation and adhesion to fibronectin. Wyczółkowska, J., Dastych, J., Slusarczyk, A., Kolago, B. J. Physiol. Pharmacol. (1994) [Pubmed]
Gene profiling in atherosclerosis reveals a key role for small inducible cytokines: validation using a novel monocyte chemoattractant protein monoclonal antibody. Lutgens, E., Faber, B., Schapira, K., Evelo, C.T., van Haaften, R., Heeneman, S., Cleutjens, K.B., Bijnens, A.P., Beckers, L., Porter, J.G., Mackay, C.R., Rennert, P., Bailly, V., Jarpe, M., Dolinski, B., Koteliansky, V., de Fougerolles, T., Daemen, M.J. Circulation (2005) [Pubmed]
Receptor-mediated modulation of murine mast cell function by alpha-melanocyte stimulating hormone. Adachi, S., Nakano, T., Vliagoftis, H., Metcalfe, D.D. J. Immunol. (1999) [Pubmed]
Bacterial products primarily mediate fibroblast inhibition in biomaterial infection. Henke, P.K., Bergamini, T.M., Watson, A.L., Brittian, K.R., Powell, D.W., Peyton, J.C. J. Surg. Res. (1998) [Pubmed]
Estrogen selectively regulates chemokines in murine splenocytes. Lengi, A.J., Phillips, R.A., Karpuzoglu, E., Ahmed, S.A. J. Leukoc. Biol. (2007) [Pubmed]
Characterization of alpha 5-integrin-dependent mast cell adhesion following Fc epsilon RI aggregation. Dastych, J., Wyczółkowska, J., Metcalfe, D.D. Int. Arch. Allergy Immunol. (2001) [Pubmed]
Fas (CD95, APO-1) antigen expression and function in murine mast cells. Hartmann, K., Wagelie-Steffen, A.L., von Stebut, E., Metcalfe, D.D. J. Immunol. (1997) [Pubmed]

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