Disease relevance of DDT

• Influence of dietary treatment on rat carcass DDT residues and toxicity parameters [1].

Psychiatry related information on DDT

• Avian species thus reflect biological magnification of BHC and DDT residues, presumably due to their food habits [2].

High impact information on DDT

• Moreover, several tumor promoters (namely TPA, phenobarbital, nafenopin, saccharin, teleocidin, benzoyl peroxide, BHT, DDT, lindane, clofibrate and melittin) stimulated DNA synthesis even when the hepatocytes were incubated in the serumless HiWoBa2000 medium, whatever its calcium concentration [3].
• We interpreted these results as indicating that DDT and possibly Aroclor 1254 may have an uncompetitive inverse agonist activity for the TSHr [4].
• Neither trypsin digestion of the extracellular domain (ECD) nor deletion of the ECD in a mutant TSHr trunk transiently transfected in COS-7 cells counteracted the inhibitory activity of DDT and Aroclor 1254 [4].
• In this study, we aimed at establishing whether two previously identified thyroid disruptors, the insecticide 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and Aroclor 1254 (a complex mixture of polychlorinated water), may inhibit thyrotropin (TSH) receptor (TSHr) activity [4].
• DDT exerted a weak inhibitory activity against forskolin in both CHO-K1 and COS-7 cells, whereas it was nil against the agonists dopamine and 5'-(N-ethyl-carboxamido)-adenosine (NECA) in CHO cells stably transfected with the dopamine D(1) receptor and in COS-7 cells transiently transfected with the adenosine type 2a receptor (A(2)a) receptor [4].

Chemical compound and disease context of DDT

• Three acaricides, namely, amitraz, chloromethiuron and DDT, did not cause any clinically detectable toxicity in captive birds during a 5-day period [5].

Biological context of DDT

• To understand the significance of the inhibitory action of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and pyrethroid insecticides on calmodulin, a universal Ca2+ binding protein, a bovine heart phosphodiesterase-calmodulin system was studied [6].
• The anti-DDT antibodies can be used for environmental analyses and lend themselves to the elucidation of the structure of the DDT binding site [7].
• A synthetic DDT derivative in which the molecular structure of DDT was completely retained was coupled to bovine serum albumin [7].
• The present study investigated the effects of the pesticides DDT, MXC, and gammaHCH on transmembrane potential, oxidative activity, cytotoxicity and ATP-induced intracellular Ca2+ release in cultured bovine oviductal cells [8].
• These results are indicative of contamination of bovine milk with pesticide residues as a result of the ban on the use of DDT and HCH in agriculture and public health programs [9].

Anatomical context of DDT

• Furthermore, both DDT and Aroclor 1254 did indeed prevent cAMP accumulation, as induced by the constitutive activity of a point mutant TSHr(I486M) transiently transfected in African green monkey kidney fibroblast (COS)-7 cells [4].
• DDT in human milk. What determines the levels [10]?
• Besides the inhibitory effect, there was a stimulatory effect on DNA synthesis in epithelial cells in the range of 28 nM to 2.8 microM DDT and in stromal cells at 2.8 and 28 nM for MXC [11].
• Species differences in the concentrations of HCB, beta-HCH, heptachlor epoxide, and DDT-related chemicals in follicular fluid were noted as well as differences in the concentrations of some pollutants within the same species [12].
• DPH had no effect on the concentration of DDT and DDE in adipose tissue; their levels declined at a rate having a half-life value of 16 days [13].

Associations of DDT with chemical compounds

• Cypermethrin was less potent than DDT, but it also affected only calmodulin [6].
• Interaction of DDT and pyrethroids with calmodulin and its significance in the expression of enzyme activities of phosphodiesterase [6].
• The analog of DDT, diphenylethylene, was inactive against bTSH-induced increase in cAMP in CHO-K1 cells stably transfected with the TSHr [4].
• This was indicated by the loss of oligomycin and 1,1,1-trichloro-2,2 bis (p-chlorophenyl) ethane (DDT) sensitivity in the presence of 50 microM FA (12-C18:1) [14].
• Furthermore, DDT was inactive against the stimulation by isoproterenol of the endogenously expressed beta(2) adrenergic receptor in COS-7 cells [4].

Other interactions of DDT

• DDT, DDE and TDE, dihydroxynaphthalene and 1-naphthol inhibited the NADH-oxidase enzyme system onthe substrate side of cytochrome c, whereas Kelthane inhibited on the oxygen side [15].

Analytical, diagnostic and therapeutic context of DDT

• In competitive solid phase radioimmunoassays, DDT concentrations as low as 10 nM or 0.0035 mg/1 were detectable [7].
• Finally, both immunoassays were applied to the detection of DDT and group of DDT-related compounds in spiked real water, soil and food samples [16].
• Two enzyme-linked immunosorbent assays (ELISA) with chemiluminescent (CL) detection for the insecticide DDT and the group of DDT-related compounds have been optimized and characterized [16].
• Residues of DDT in man, dairy cows and soil were about five to one hundred times higher than in the control groups, while residues found in samples from the marine biota were the same or moderately higher (Figure 3) [17].
• Fifty samples of human breast milk were analysed by gas chromatography and thin layer chromatography for DDT (dichlordiphenyltrichloraethan), hexachlorobenzol, benezene-hexachlorides, dieldrin, aldrin and heptachlorepoxide [18].

References