Disease relevance of NANOS1

• In the context of brain ischemia, the activity of NOS 1 and NOS 2 is broadly deleterious, and their inhibition or inactivation is neuroprotective [1].
• The effects of ischemia on the activity of NOS 1, a Ca2+-dependent enzyme, are thought to be secondary to reversal of glutamate reuptake at synapses, activation of NMDA receptors, and resulting elevation of intracellular Ca2+ [1].
• Indeed, expressions of NOS-1 and NOS-3 were frequently observed in EBV associated GCs [2].
• Concomitant to muscle atrophy, reduced NOS 1 protein and immunostaining was found in VL not in SOL biopsies [3].
• CONCLUSION: Expression of NOS1 can not serve as a marker for highly malignant tumours in the non-small cell lung carcinomas [4].

High impact information on NANOS1

• Among those, homologs to forkhead, emx, aristaless, goosecoid, brachyury, wnt and nanos genes are regulated during apical patterning in cnidarians, suggesting that key components of early organizer activity were conserved across evolution and recruited for either anterior, axial, or dorso-ventral patterning in bilaterians [5].
• Functional redundancy among Nanos proteins and a distinct role of Nanos2 during male germ cell development [6].
• E-Cadherin Regulates Human Nanos1, which Interacts with p120ctn and Induces Tumor Cell Migration and Invasion [7].
• However, the production of nitric oxide in blood vessels by NOS 3, which, like NOS 1, is Ca2+-dependent, causes vasodilatation and improves blood flow in the penumbral region of brain infarcts [1].
• Translational repression: a duet of Nanos and Pumilio [8].

Chemical compound and disease context of NANOS1

• A novel cultured cell line NOS-1, derived from a human oral primary squamous cell carcinoma (SCC) of the lower gingiva, was established without xenografting the tumor into nude mice by means of "Geneticin" treatment, which allowed for elimination of contaminated fibroblasts and produced enriched tumor cells at an early stage of the culture [9].

Biological context of NANOS1

• We found that vasa and nanos family genes are expressed not only in presumptive PGCs late in embryonic development, but also in multiple somatic cell types during early embryogenesis [10].

Anatomical context of NANOS1

• These results suggest the possibility that the interaction of PUM2 and NOS1 may play a conserved role in germ cell development and maintenance in humans as in model organisms [11].
• We also show that in men, the NOS1 and PUM2 proteins are particularly abundant in germline stem cells [11].
• Dramatically lower NOS 1 immunoreactivity was observed in tumour vascular endothelium (1-3% and 15-20% in tumour and normal groups, respectively) [12].
• The keratinocytes, which make up the bulk of the epidermis, constitutively express the neuronal isoform of NO synthase (NOS1), whereas the fibroblasts in the dermis and other cell types in the skin express the endothelial isoform (NOS3) [13].
• NOS-1 is localised primarily to neuronal structures, where NO is a mediator of the inhibitory Non-Adrenergic Non-Cholinergic System and NOS-3 is present in endothelial cells [14].

Associations of NANOS1 with chemical compounds

• Differences between NOS 1 and NOS 2 production of .NO, O2.-, and H2O2 may define the specific physiologic function of each isozyme [15].
• In the absence of L-arginine, however, NOS 1 generation of O2.- and H2O2 was found to be substantially greater than that measured for NOS 2 [15].
• Exposure to Cd increased the levels of hypothalamic NOS1 mRNA at 09:00 hr and decreased the levels of NOS2 mRNA at 01:00 hr, with melatonin treatment preventing Cd effects [16].
• Paraffin-embedded tissues were stained with hematoxylin and eosin, or with antibodies to NOS1 and p53, and evaluated under light microscope [4].
• The model, its strengths and its weaknesses are discussed with reference to four examples of functional ncRNA sequences: a riboswitch (guanine), a zipcode (nanos), a splicing factor (U4) and a ribozyme (RNase P) [17].

Other interactions of NANOS1

• We isolated human Nanos1 as a transcript of which levels decrease on E-cadherin reexpression in a human breast cancer cell line [7].
• The translational regulation of the mRNAs encoding cyclin B1, Oskar, Nanos, Male specific lethal 2 (Msl-2), lipoxygenase and Lin-14 is discussed [18].

Analytical, diagnostic and therapeutic context of NANOS1

• Our aim was to compare NOS1 expression in non-small lung carcinomas with respect to tumor staging and p53 protein expression [4].
• MVs were isolated from NOS-1 cells using a collagenase digestion and ultracentrifugation method [19].

References