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Gene Review

F12  -  coagulation factor XII (Hageman factor)

Sus scrofa

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High impact information on F12


Biological context of F12


Anatomical context of F12

  • At the end of the 120-h culture period, 30-50% of the spermatogonia were viable in KSOM, whereas in DMEM/F12 very few cells survived [7].
  • Dog gingival explants, 1 X 2 mm, were cultured on the cementum surfaces of pig root pieces in a defined medium consisting of DMEM and F12 (1V/1V), transferrin, insulin, epidermal growth factor, cortisone, selenium, and high-density lipoprotein [8].
  • Here, we have systematically characterized pig IPP follicular lymphocytes and show that about 90% B cells that are positive for surface immunoglobulin G (sIgM+) and express an immature phenotype characterized by expression of myeloid marker sWC3 (74-22-15) and two molecules recognized by IPP B-cell-specific monoclonal antibodies (F10/4, F12/35) [5].
  • The distended intestinal sac method was used to isolate 12 sequential fractions (F1 through F12) of epithelial cells [9].
  • Porcine granulosa cells from small (<3 mm), medium (3-5 mm) and large (>5 mm) follicles were seeded at different densities in DMEM:Ham's F12 (1:1) with or without different concentrations of VEGF or bFGF [10].

Associations of F12 with chemical compounds

  • The cells were cultured for up to 120 h in Dulbecco's modified Eagle's medium/Ham's F-12 medium (DMEM/F12) or a potassium-rich medium derived by the simplex optimization method (KSOM) [7].
  • Porcine preadipocytes were cultured in serum-free medium (DME/F12 medium containing 100 nM insulin, 10 micrograms/mL transferrin, and 50 ng/mL hydrocortisone) [11].
  • Other serine proteinases such as factor Xa, factor XII, thrombin and plasmin do not hydrolyze B. bauhinioides inhibitor related substrates [12].
  • Similarly, the prothrombin complex, antithrombin, thrombin-antithrombin complex, factor XII, and the urinary excretion of 2,3-dinor-thromboxane B2 were not significantly altered [13].
  • Activation of factor XII and prekallikrein with cholesterol sulfate [14].

Other interactions of F12


Analytical, diagnostic and therapeutic context of F12

  • When tested with antiserum against other common nematodes of pigs from China, the APF was found to be markedly more specific than S3 antigens (prepared by a combination of cell fractionation and differential centrifugation according to Despommier and Lacetti, 1981) and fractions produced by Sephacryl S-200 gel filtration (F1 to F12) [16].
  • When porcine plasma was subjected to four steps of ion-exchange column chromatographies, factor XII (F. XII) was separated into two fractions, F [17].


  1. Sulfated glycosaminoglycans of guinea pig basophilic leukocytes. Orenstein, N.S., Galli, S.J., Dvorak, A.M., Silbert, J.E., Dvorak, H.F. J. Immunol. (1978) [Pubmed]
  2. Paradoxical release of insulin by adult pig islets in vitro. Recovery after culture in a defined tissue culture medium. Davalli, A.M., Bertuzzi, F., Socci, C., Scaglia, L., Gavazzi, F., Freschi, M., DiCarlo, V., Pontiroli, A.E., Pozza, G. Transplantation (1993) [Pubmed]
  3. Surfactant protein B: disulfide bridges, structural properties, and kringle similarities. Johansson, J., Curstedt, T., Jörnvall, H. Biochemistry (1991) [Pubmed]
  4. Porcine endometrial expression of kininogen, factor XII, and plasma kallikrein in cyclic and pregnant gilts. Vonnahme, K.A., Fernando, S.C., Ross, J.W., Ashworth, M.D., DeSilva, U., Malayer, J.R., Geisert, R.D. Biol. Reprod. (2004) [Pubmed]
  5. Systematic characterization of porcine ileal Peyer's patch, I. apoptosis-sensitive immature B cells are the predominant cell type. Andersen, J.K., Takamatsu, H., Oura, C.A., Brookes, S.M., Pullen, L., Parkhouse, R.E. Immunology (1999) [Pubmed]
  6. Identification of components of the intrafollicular bradykinin-producing system in the porcine ovary. Kihara, T., Kimura, A., Moriyama, A., Ohkubo, I., Takahashi, T. Biol. Reprod. (2000) [Pubmed]
  7. Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM. Dirami, G., Ravindranath, N., Pursel, V., Dym, M. Biol. Reprod. (1999) [Pubmed]
  8. The effects of partial demineralization and fibronectin on migration and growth of gingival epithelial cells on cementum in vitro. Pitaru, S., Hekmati, M., Geiger, S., Savion, N. J. Dent. Res. (1988) [Pubmed]
  9. Enterocyte digestive enzyme activity along the crypt-villus and longitudinal axes in the neonatal pig small intestine. Fan, M.Z., Stoll, B., Jiang, R., Burrin, D.G. J. Anim. Sci. (2001) [Pubmed]
  10. Effects of VEGF and bFGF on proliferation and production of steroids and nitric oxide in porcine granulosa cells. Grasselli, F., Basini, G., Bussolati, S., Tamanini, C. Reprod. Domest. Anim. (2002) [Pubmed]
  11. Effect of retinoic acid on differentiation of cultured pig preadipocytes. Suryawan, A., Hu, C.Y. J. Anim. Sci. (1997) [Pubmed]
  12. Synthetic peptides and fluorogenic substrates related to the reactive site sequence of Kunitz-type inhibitors isolated from Bauhinia: interaction with human plasma kallikrein. Oliva, M.L., Santomauro-Vaz, E.M., Andrade, S.A., Juliano, M.A., Pott, V.J., Sampaio, M.U., Sampaio, C.A. Biol. Chem. (2001) [Pubmed]
  13. Twenty-four-hour heparin-free veno-right ventricular ECMO: an experimental study. Koul, B., Vesterqvist, O., Egberg, N., Steen, S. Ann. Thorac. Surg. (1992) [Pubmed]
  14. Activation of factor XII and prekallikrein with cholesterol sulfate. Shimada, T., Kato, H., Iwanaga, S., Iwamori, M., Nagai, Y. Thromb. Res. (1985) [Pubmed]
  15. Colonial growth and differentiation of epithelial cells derived from abattoir adult porcine livers. Kano, J., Tokiwa, T., Zhou, X., Kodama, M. J. Gastroenterol. Hepatol. (1998) [Pubmed]
  16. Specificity of affinity-purified Trichinella spiralis antigens. Chan, S.W., Ko, R.C. Vet. Parasitol. (1992) [Pubmed]
  17. Studies on factor XII in porcine plasma: purification and its conversion to activated form by porcine plasma kallikrein. Mashiko, H., Kato, K., Fujii, K., Takahashi, H. Biochim. Biophys. Acta (1996) [Pubmed]
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