Disease relevance of TRPV2

• Our data suggest the existence of a common activation mechanism for TRPV1, TRPV2, and TRPV3 that may serve as a therapeutic target for pain management and treatment for diseases caused by hypersensitivity and temperature misregulation [1].
• It has been demonstrated that TRPV2 is activated by moderate thermal stimuli and, in the rat, is expressed in medium to large diameter dorsal root ganglion neurons [2].
• These data may also implicate the involvement of TRPV2 in disorders of the hypothalamic-pituitary-adrenal axis, including anxiety, depression, hypertension, and preterm labor [2].
• The performance of VRL is also evaluated for classifying subjects with normal sinus rhythm and congestive heart failure using the RR intervals taken from the PhysioNet database [3].

Psychiatry related information on TRPV2

• The translated gene shares 46% and 43% identity with VR1 and VRL-1, respectively, and maps to chromosome 12q23-24.1, a locus associated with bipolar affective disorder [4].

High impact information on TRPV2

• TRPV3 is a temperature-sensitive vanilloid receptor-like protein [5].
• Here we identify a structurally related receptor, VRL-1, that does not respond to capsaicin, acid or moderate heat [6].
• Instead, VRL-1 is activated by high temperatures, with a threshold of approximately 52 degrees C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium- to large-diameter neurons, making it a candidate receptor for transducing high-threshold heat responses in this class of cells [6].
• We describe the functional coupling of TRPV2 protein to calcium fluxes and proinflammatory degranulation events in mast cells [7].
• 2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3 [1].

Biological context of TRPV2

• Using reverse-transcription polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR (qRT-PCR), TRPV1 and TRPV2 mRNA was detected in mRNA isolated from human whole peripheral blood [8].
• The homologous receptor TRPV2 shows wide tissue distribution including some sensory neurons, where it is proposed to function as a heat sensor or a growth-factor-activated channel [9].
• Formation of the TRPV2/RGA complex is dependent upon a cellular glycosylation event, suggesting that RGA may play a chaperone or targeting role for TRPV2 during the maturation of the ion channel protein [10].

Anatomical context of TRPV2

• A-delta fibres exhibit properties that may be explained by TRPV2 containing channels which is present in large diameter sensory neurons that do not express TRPV1 [11].
• This is the first study to demonstrate the presence of both TRPV1 and TRPV2 in human peripheral lymphocytes [8].
• The present study demonstrates both gene and protein expression of TRPV1 and TRPV2 in human peripheral blood cells (PBCs) using molecular and immunocytochemical techniques [8].
• Immunohistochemical labeling detected TRPV1 and TRPV2 proteins in nodose ganglia [12].
• We extended the search for TRPV1-TRPV2 co-localization in the brain, where we detected extensive co-expression of the two receptors in the IV, V and VI layer neurons of the adult rat cerebral cortex [9].

Associations of TRPV2 with chemical compounds

• The vanilloid receptor family of cation channels includes the capsaicin-sensitive, proton- and heat-activated TRPV1 and noxious heat-activated TRPV2 [8].
• These results suggest that fMLP induces translocation of TRPV2 from intracellular compartment to the plasma membrane, and this translocation is critical for fMLP-induced calcium entry [13].
• Few, if any, cells in these regions expressed TRPV2 immunoreactivity in the absence of oxytocin immunoreactivity or vasopressin immunoreactivity [2].

Other interactions of TRPV2

• Data from the study of mice in which TRPV2, TRPV3 or TRPV4 have been deleted are also eagerly awaited [11].
• Co-expression of the voltage-gated potassium channel Kv1.4 with transient receptor potential channels (TRPV1 and TRPV2) and the cannabinoid receptor CB1 in rat dorsal root ganglion neurons [14].
• We examined transient receptor potential (TRP) V1 and TRPV2 expression in calcitonin gene-related peptide (CGRP) positive (+) primary sensory neurons projecting to the skin and skeletal muscles of the rat dorsum [15].
• Immunological analysis demonstrated that this isoform is a 56-kDa protein, which is localized predominantly in the Golgi apparatus in cardiomyocytes and C2C12 myoblasts/myotubes, and we found that two SR domains were required both for Golgi targeting and for interaction with TRPV2 [16].
• We performed immunohistochemistry for kit, connexin 43 and VRL1/TRPV2 [17].

Analytical, diagnostic and therapeutic context of TRPV2

• We identified a novel SR-containing protein, named GSRP-56 (Golgi-localized SR-containing protein-56), by a yeast two-hybrid method, using a member of the transient receptor potential channel family, TRPV2, as bait [16].
• Here, we examined expression patterns of the voltage-gated potassium channel Kv1.4, the nociceptive transduction channels TRPV1 and TRPV2 as well as the putative anti-nociceptive cannabinoid receptor CB1 by immunofluorescence double-labelings in sections of rat dorsal root ganglia (DRGs) [14].
• When CB1-mRNA was detected using in situ hybridizations an additional subset of larger neurons was labeled including 82.4+/-17.7% of the TRPV2 expressing neurons [14].
• Isolates examined by pulsed-field gel electrophoresis were indistinguishable from the Latin American epidemic strain, C6707; however, they differed significantly from the endemic Gulf Coast strain (VRL 1984), the sixth-pandemic strain (569-B), and a V. cholerae non-O1 strain isolated from a ship arriving from a foreign port [18].

References