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Gene Review

RBM  -  similar to RNA binding motif protein, Y...

Homo sapiens

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Disease relevance of RBM


High impact information on RBM

  • Expression of RBM in the nuclei of human germ cells is dependent on a critical region of the Y chromosome long arm [5].
  • The distribution of RBM protein in the adult correlates with the pattern of transcriptional activity in spermatogenesis, suggesting that RBM is involved in the nuclear metabolism of newly synthesized RNA [5].
  • In the absence of detectable RBM expression we see stages of germ cell development up to early meiosis, but not past this point into the haploid phase of spermatogenesis [5].
  • We conclude that the hnRNP G family of proteins is involved in pre-mRNA splicing and infer that RBM may be involved in Tra2beta-dependent splicing in spermatocytes [6].
  • RBM and Tra2beta co-localize in two major domains in human spermatocyte nuclei [6].

Biological context of RBM

  • Degeneracy in human multicopy RBM (YRRM), a candidate spermatogenesis gene [1].
  • We have used PCR followed by SSCP analysis to map RBM on a Y Chromosome (Chr) YAC contig and have demonstrated a distribution that spans a major part of this chromosome's euchromatin [1].
  • Incubation with the protein interaction domain of RBM inhibited splicing in vitro of a specific pre-mRNA substrate containing an essential enhancer bound by Tra2beta [6].
  • Moreover, pre-mRNA splicing components are targeted to the same punctate nuclear regions as RBM during the early stages of germ cell development but late in meiosis this spatial association breaks down [7].
  • The second strategy used subcloning of the YAC clone 925D10 (that contained the RBM gene described here) into cosmids [8].

Anatomical context of RBM

  • We show here that RBM, hnRNP G and a novel testis-specific relative, termed hnRNP G-T, interact with Tra2beta, an activator of pre-mRNA splicing that is ubiquitous but highly expressed in testis [6].
  • The authors analyzed the expression of the RBM gene in germ cell tumors and in the seminiferous tubules in the vicinity of these tumors to identify the presence of IGCN [9].
  • We examined the effect of three different subcellular matrices, plastic, type I collagen, and reconstituted basement membrane-like material (RBM), on the synthesis of sulfated glycosaminoglycans by cultured IMR-90 human lung fibroblasts [10].
  • These findings highlight the intrinsic interpretative difficulties of normal PCR analysis for DAZ and RBM on leukocytes and suggest caution in the use of germ cells for assisted reproductive techniques in these cases to avoid transmission of genetic abnormalities to male offspring [11].
  • However, on gels (3-dimensional substrata) reconstituted from a basement membrane extract (RBM), PC12 cells attach extending short processes transiently and within one day, form networks of small aggregates interconnected by process-bearing cells [12].

Associations of RBM with chemical compounds


Other interactions of RBM

  • In all, 39 potential exons were isolated, including 2 exons from the DAZ gene, 3 exons from the BPY2 gene, 2 exons from the PRY gene, and 1 exon from a member of the RBM II gene family; all these genes have been shown previously to map to the AZFc region [14].
  • One patient, who was previously shown to be azoospermia factor region (AZF)b deleted, lacked RBM mRNA and presented with reduced amplification of protamine-2 mRNA [15].
  • While pre-mRNA splicing components undergo profound spatial reorganisations during spermatogenesis, neither heterogeneous ribonucleoproteins nor the transcription factor Sp1 show either developmental spatial reorganisations or any specific co-localisation with RBM [7].
  • We analysed, by reverse transcription-polymerase chain reaction (RT-PCR), the expression of DAZ, RBM and SRY genes, in testicular cells from infertile men affected by idiopathic severe hypospermatogenesis, obstructive azoospermia and Sertoli cell-only syndrome [11].
  • These results disclosed a wide Y long arm deletion, including all hypothized Yq azoospermia loci (except for AZFa and probably for one of the RBM genes, which lie proximally to the deletion) and possibly the Y-specific growth control region (GCY), mapped between DYS11 and DYS246 loci [16].

Analytical, diagnostic and therapeutic context of RBM


  1. Degeneracy in human multicopy RBM (YRRM), a candidate spermatogenesis gene. Prosser, J., Inglis, J.D., Condie, A., Ma, K., Kerr, S., Thakrar, R., Taylor, K., Cameron, J.M., Cooke, H.J. Mamm. Genome (1996) [Pubmed]
  2. Deletion of RBM and DAZ in azoospermia: evaluation by PRINS. Kadandale, J.S., Wachtel, S.S., Tunca, Y., Martens, P.R., Wilroy, R.S., Tharapel, A.T. Am. J. Med. Genet. (2002) [Pubmed]
  3. Positive correlation between the expression of X-chromosome RBM genes (RBMX, RBM3, RBM10) and the proapoptotic Bax gene in human breast cancer. Martínez-Arribas, F., Agudo, D., Pollán, M., Gómez-Esquer, F., Díaz-Gil, G., Lucas, R., Schneider, J. J. Cell. Biochem. (2006) [Pubmed]
  4. The incidence of bacterial meningitis in the Netherlands--a comparison of three registration systems, 1977-1982. Spanjaard, L., Bol, P., Ekker, W., Zanen, H.C. J. Infect. (1985) [Pubmed]
  5. Expression of RBM in the nuclei of human germ cells is dependent on a critical region of the Y chromosome long arm. Elliott, D.J., Millar, M.R., Oghene, K., Ross, A., Kiesewetter, F., Pryor, J., McIntyre, M., Hargreave, T.B., Saunders, P.T., Vogt, P.H., Chandley, A.C., Cooke, H. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  6. RBMY, a probable human spermatogenesis factor, and other hnRNP G proteins interact with Tra2beta and affect splicing. Venables, J.P., Elliott, D.J., Makarova, O.V., Makarov, E.M., Cooke, H.J., Eperon, I.C. Hum. Mol. Genet. (2000) [Pubmed]
  7. Dynamic changes in the subnuclear organisation of pre-mRNA splicing proteins and RBM during human germ cell development. Elliott, D.J., Oghene, K., Makarov, G., Makarova, O., Hargreave, T.B., Chandley, A.C., Eperon, I.C., Cooke, H.J. J. Cell. Sci. (1998) [Pubmed]
  8. Genomic structure of a Y-specific ribonucleic acid binding motif-containing gene: a putative candidate for a subset of male infertility. Najmabadi, H., Chai, N., Kapali, A., Subbarao, M.N., Bhasin, D., Woodhouse, E., Yen, P., Bhasin, S. J. Clin. Endocrinol. Metab. (1996) [Pubmed]
  9. Absence of RBM expression as a marker of intratubular (in situ) germ cell neoplasia of the testis. Lifschitz-Mercer, B., Elliott, D.J., Leider-Trejo, L., Schreiber-Bramante, L., Hassner, A., Eisenthal, A., Maymon, B.B. Hum. Pathol. (2000) [Pubmed]
  10. Effect of subcellular matrix on glycosaminoglycan synthesis by human lung fibroblasts. Cui, D.J., Dubaybo, B.A., Durr, R.A., Thet, L.A. J. Appl. Physiol. (1987) [Pubmed]
  11. Absence of testicular DAZ gene expression in idiopathic severe testiculopathies. Ferlin, A., Moro, E., Onisto, M., Toscano, E., Bettella, A., Foresta, C. Hum. Reprod. (1999) [Pubmed]
  12. The extracellular matrix modulates the response of PC12 cells to nerve growth factor: cell aggregation versus neurite outgrowth on 3-dimensional laminin substrata. Keshmirian, J., Bray, G., Carbonetto, S. J. Neurocytol. (1989) [Pubmed]
  13. Corrosion resistance and biocompatibility of a new porous surface for titanium implants. Simon, M., Lagneau, C., Moreno, J., Lissac, M., Dalard, F., Grosgogeat, B. Eur. J. Oral Sci. (2005) [Pubmed]
  14. An exon map of the AZFc male infertility region of the human Y chromosome. Wong, J., Blanco, P., Affara, N.A. Mamm. Genome (1999) [Pubmed]
  15. A preliminary report on the implication of RT-PCR detection of DAZ, RBMY1, USP9Y and Protamine-2 mRNA in testicular biopsy samples from azoospermic men. Friel, A., Houghton, J.A., Glennon, M., Lavery, R., Smith, T., Nolan, A., Maher, M. Int. J. Androl. (2002) [Pubmed]
  16. Short stature and azoospermia in a patient with Y chromosome long arm deletion. De Rosa, M., De Brasi, D., Zarrilli, S., Paesano, L., Pivonello, R., D'Agostino, A., Longobardi, S., Merola, B., Lupoli, G., Ogata, T., Lombardi, G. J. Endocrinol. Invest. (1997) [Pubmed]
  17. Multiple functional copies of the RBM gene family, a spermatogenesis candidate on the human Y chromosome. Chai, N.N., Salido, E.C., Yen, P.H. Genomics (1997) [Pubmed]
  18. High-resolution fluorescence in situ hybridization of RBM- and TSPY-related cosmids on released Y chromatin in humans and pygmy chimpanzees. Conrad, C., Hierl, T., Gläser, B., Taylor, K., Zeitler, S., Chandley, A.C., Schempp, W. Chromosome Res. (1996) [Pubmed]
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