Disease relevance of Primed In Situ Labeling

• By PRINS we identified the SRY gene in two XX males, a woman with XY gonadal dysgenesis, and an azoospermic male with Xp-Yp interchange [1].
• Deletion of RBM and DAZ in azoospermia: evaluation by PRINS [2].
• Primed in situ amplification (PRINS) and cycle PRINS were used to detect, via the incorporation of a fluorescein labelled nucleotide, the presence of specific genes carried on both high and low copy number plasmids in individual cells of Escherichia coli and a marine bacterium, SW5 [3].
• Recent advances in molecular cytogenetics, such as comparative genomic hybridization, the primed in-situ labeling technique, the development of new telomeric probes and spectral karyotyping, are being evaluated for their role in the prenatal diagnosis of chromosomal abnormalities [4].
• We report two cases of acute myeloid leukemia (AML) with tetrasomy 8 detected in patients' bone marrow samples using chromosome GTG-banding, fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS) techniques [5].

Psychiatry related information on Primed In Situ Labeling

• Sixty-five children with unexplained mental retardation were studied using PRINS technology; two of them were shown to have a telomeric deletion [6].

High impact information on Primed In Situ Labeling

• To examine this variation, a new method was developed to combine primed in situ labeling to label centromeres of one chromosome category and immunostaining of tubulin [7].
• Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS [8].
• Highly stereoselective prins cyclization of silylmethyl-substituted cyclopropyl carbinols to 2,4,6-trisubstituted tetrahydropyrans [9].
• Aromatic 4-tetrahydropyranyl and 4-quinuclidinyl cations. Linking Prins with Cope and Grob [10].
• This was considerably reduced by preincubation of the cells in lysine and an excess of cold nucleotides prior to the PRINS reaction [11].

Biological context of Primed In Situ Labeling

• The absence of telomeric sequences in the ring chromosome was demonstrated by multicolour PRINS: a three-step PRimed IN Situ labelling technique, using unlabelled primers [12].
• Chromosome enumeration by cycling PRINS is demonstrated using primers for repeat DNA sequences, whilst single copy sequence detection is demonstrated using bcl-2, CFTR and chromosome 21 specific primer pairs in ISPCR [13].
• In this study, primed in situ labeling (PRINS), fluorescence in situ hybridization (FISH) and conventional cytogenetic approaches were used to investigate 54 cases of hematopoietic disorders [14].
• Primed in situ DNA labelling (PRINS) labels the telomeres of Avena, Triticum, Secale, Hordeum, Lolium, Festuca and Trifolium when primers are used that correspond to the repeat unit of Arabidopsis telomeres [15].
• Additionally, PRINS with primers enabling amplification of 5S rDNA and CMA3 staining revealed existence of 5S rDNA sites located close to GC rich regions [16].

Anatomical context of Primed In Situ Labeling

• A total of 183 PRINS reactions were performed with primers for chromosomes 13, 16, 18, 21 or X on 63 I-PBs removed from oocytes that failed to become fertilized during IVF [17].

Associations of Primed In Situ Labeling with chemical compounds

• [reaction: see text] Ten acyclic and monocyclic delta,epsilon-unsaturated ketones, with and without methyl substituents on the double bond, underwent halide-terminated Prins (halo-Prins) cyclizations under anhydrous conditions in the presence of Lewis acids [18].
• The first total synthesis of the reported structure of the sponge metabolite clavosolide A is described using a Prins cyclisation to assemble the tetrahydropyran core followed by manipulation of the side-chain, dimerisation and finally glycosidation [19].
• MCM-41 was found to be an active heterogeneous catalyst for the synthesis of nopol by the Prins condensation of beta-pinene and paraformaldehyde, but Sn-MCM-41 in which Sn has been grafted on MCM-41 by chemical vapor deposition is far more active and combines high efficiency and recyclability [20].
• By contrast, Prins cyclization of 6a-e catalyzed by hydrochloric acid at low temperatures affords cis products 7a-e with diastereomeric ratios of up to 98:2 [21].
• Mesoporous iron phosphate is found to be a highly active and recyclable heterogeneous catalyst for the selective synthesis of nopol by Prins condensation of beta-pinene and paraformaldehyde in acetonitrile at 80 degrees C [22].

Gene context of Primed In Situ Labeling

• To assess the utility and efficiency of the PRINS method in the detection of RB1 and p53 deletions, we evaluated 10 patients with hematological disorders and known rearrangements, i.e., deletions involving 13q14 and 17p13 regions [23].
• Prader Willi/Angelman and DiGeorge/velocardiofacial syndrome deletions: diagnosis by primed in situ labeling (PRINS) [24].
• With PRINS, we physically localized the SRY gene to Yp11.31p11.32 and the SOX3 gene to Xq26q27 [25].
• A primer pair based on the bovine SOD1 gene (Barendse et al., 1994b) was used for the DISC-PCR procedure [26].
• To solve this problem, we developed a double-strand PRINS technique, which uses two primers, (TTAGGG)(7) and (CCCTAA)(7), to label both forward and reverse telomeric DNA strands [27].

Analytical, diagnostic and therapeutic context of Primed In Situ Labeling

• We investigated cervical biopsies (n = 20) with morphological signs of HPV infection and found that the PRINS method is at least as sensitive as a classical in situ hybridization assay for detecting HPV DNA in paraffin-embedded, formalin-fixed tissue [28].

References