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MeSH Review

Primed In Situ Labeling

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Disease relevance of Primed In Situ Labeling


Psychiatry related information on Primed In Situ Labeling

  • Sixty-five children with unexplained mental retardation were studied using PRINS technology; two of them were shown to have a telomeric deletion [6].

High impact information on Primed In Situ Labeling

  • To examine this variation, a new method was developed to combine primed in situ labeling to label centromeres of one chromosome category and immunostaining of tubulin [7].
  • Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS [8].
  • Highly stereoselective prins cyclization of silylmethyl-substituted cyclopropyl carbinols to 2,4,6-trisubstituted tetrahydropyrans [9].
  • Aromatic 4-tetrahydropyranyl and 4-quinuclidinyl cations. Linking Prins with Cope and Grob [10].
  • This was considerably reduced by preincubation of the cells in lysine and an excess of cold nucleotides prior to the PRINS reaction [11].

Biological context of Primed In Situ Labeling


Anatomical context of Primed In Situ Labeling

  • A total of 183 PRINS reactions were performed with primers for chromosomes 13, 16, 18, 21 or X on 63 I-PBs removed from oocytes that failed to become fertilized during IVF [17].

Associations of Primed In Situ Labeling with chemical compounds

  • [reaction: see text] Ten acyclic and monocyclic delta,epsilon-unsaturated ketones, with and without methyl substituents on the double bond, underwent halide-terminated Prins (halo-Prins) cyclizations under anhydrous conditions in the presence of Lewis acids [18].
  • The first total synthesis of the reported structure of the sponge metabolite clavosolide A is described using a Prins cyclisation to assemble the tetrahydropyran core followed by manipulation of the side-chain, dimerisation and finally glycosidation [19].
  • MCM-41 was found to be an active heterogeneous catalyst for the synthesis of nopol by the Prins condensation of beta-pinene and paraformaldehyde, but Sn-MCM-41 in which Sn has been grafted on MCM-41 by chemical vapor deposition is far more active and combines high efficiency and recyclability [20].
  • By contrast, Prins cyclization of 6a-e catalyzed by hydrochloric acid at low temperatures affords cis products 7a-e with diastereomeric ratios of up to 98:2 [21].
  • Mesoporous iron phosphate is found to be a highly active and recyclable heterogeneous catalyst for the selective synthesis of nopol by Prins condensation of beta-pinene and paraformaldehyde in acetonitrile at 80 degrees C [22].

Gene context of Primed In Situ Labeling

  • To assess the utility and efficiency of the PRINS method in the detection of RB1 and p53 deletions, we evaluated 10 patients with hematological disorders and known rearrangements, i.e., deletions involving 13q14 and 17p13 regions [23].
  • Prader Willi/Angelman and DiGeorge/velocardiofacial syndrome deletions: diagnosis by primed in situ labeling (PRINS) [24].
  • With PRINS, we physically localized the SRY gene to Yp11.31p11.32 and the SOX3 gene to Xq26q27 [25].
  • A primer pair based on the bovine SOD1 gene (Barendse et al., 1994b) was used for the DISC-PCR procedure [26].
  • To solve this problem, we developed a double-strand PRINS technique, which uses two primers, (TTAGGG)(7) and (CCCTAA)(7), to label both forward and reverse telomeric DNA strands [27].

Analytical, diagnostic and therapeutic context of Primed In Situ Labeling

  • We investigated cervical biopsies (n = 20) with morphological signs of HPV infection and found that the PRINS method is at least as sensitive as a classical in situ hybridization assay for detecting HPV DNA in paraffin-embedded, formalin-fixed tissue [28].


  1. Localization of SRY by primed in situ labeling in XX and XY sex reversal. Kadandale, J.S., Wachtel, S.S., Tunca, Y., Wilroy, R.S., Martens, P.R., Tharapel, A.T. Am. J. Med. Genet. (2000) [Pubmed]
  2. Deletion of RBM and DAZ in azoospermia: evaluation by PRINS. Kadandale, J.S., Wachtel, S.S., Tunca, Y., Martens, P.R., Wilroy, R.S., Tharapel, A.T. Am. J. Med. Genet. (2002) [Pubmed]
  3. Improved methods for in situ enzymatic amplification and detection of low copy number genes in bacteria. Jacobs, D., Angles, M.L., Goodman, A.E., Neilan, B.A. FEMS Microbiol. Lett. (1997) [Pubmed]
  4. Applied molecular genetic techniques for prenatal diagnosis. Van den Veyver, I.B., Roa, B.B. Current opinion in obstetrics & gynecology. (1998) [Pubmed]
  5. Tetrasomy 8 is associated with a major cellular proliferative advantage and a poor prognosis. two cases of myeloid hematologic disorders and review of the literature. Yan, J., Marceau, D., Drouin, R. Cancer Genet. Cytogenet. (2001) [Pubmed]
  6. Use of primed in situ labeling (PRINS) for the detection of telomeric deletions associated with mental retardation. Bonifacio, S., Centrone, C., Da Prato, L., Scordo, M.R., Estienne, M., Torricelli, F. Cytogenet. Cell Genet. (2001) [Pubmed]
  7. Asymmetrical segregation of chromosomes with a normal metaphase/anaphase checkpoint in polyploid megakaryocytes. Roy, L., Coullin, P., Vitrat, N., Hellio, R., Debili, N., Weinstein, J., Bernheim, A., Vainchenker, W. Blood (2001) [Pubmed]
  8. Identification and characterization of a novel, psoriasis susceptibility-related noncoding RNA gene, PRINS. Sonkoly, E., Bata-Csorgo, Z., Pivarcsi, A., Polyanka, H., Kenderessy-Szabo, A., Molnar, G., Szentpali, K., Bari, L., Megyeri, K., Mandi, Y., Dobozy, A., Kemeny, L., Szell, M. J. Biol. Chem. (2005) [Pubmed]
  9. Highly stereoselective prins cyclization of silylmethyl-substituted cyclopropyl carbinols to 2,4,6-trisubstituted tetrahydropyrans. Yadav, V.K., Kumar, N.V. J. Am. Chem. Soc. (2004) [Pubmed]
  10. Aromatic 4-tetrahydropyranyl and 4-quinuclidinyl cations. Linking Prins with Cope and Grob. Alder, R.W., Harvey, J.N., Oakley, M.T. J. Am. Chem. Soc. (2002) [Pubmed]
  11. Flow cytometric quantitation of sequence-specific mRNA in hemopoietic cell suspensions by primer-induced in situ (PRINS) fluorescent nucleotide labeling. Bains, M.A., Agarwal, R., Pringle, J.H., Hutchinson, R.M., Lauder, I. Exp. Cell Res. (1993) [Pubmed]
  12. Ring chromosome 20 with loss of telomeric sequences detected by multicolour PRINS. Brandt, C.A., Kierkegaard, O., Hindkjaer, J., Jensen, P.K., Pedersen, S., Therkelsen, A.J. Clin. Genet. (1993) [Pubmed]
  13. In situ polymerase chain reaction and cycling primed in situ amplification: improvements and adaptations. Paskins, L., Brownie, J., Bull, J. Histochem. Cell Biol. (1999) [Pubmed]
  14. Trisomy 8 and monosomy 7 detected in bone marrow using primed in situ labeling, fluorescence in situ hybridization, and conventional cytogenetic analyses. A study of 54 cases with hematological disorders. Yan, J., Zhang, X.X., Fetni, R., Drouin, R. Cancer Genet. Cytogenet. (2001) [Pubmed]
  15. Labelling telomeres of cereals, grasses and clover by primed in situ DNA labelling. Thomas, H.M., Williams, K., Harper, J.A. Chromosome Res. (1996) [Pubmed]
  16. Cytogenetic analysis of platyfish (Xiphophorus maculatus) shows location of major and minor rDNA on chromosomes. Ocalewicz, K. Hereditas (2004) [Pubmed]
  17. Use of PRINS for preconception screening of polar bodies for common aneuploidies. Petit, C., Martel-Petit, V., Fleurentin, A., Monnier-Barbarino, P., Jonveaux, P., Gerard, H. Prenat. Diagn. (2000) [Pubmed]
  18. Syn- and anti-selective Prins cyclizations of delta,epsilon-unsaturated ketones to 1,3-halohydrins with Lewis acids. Miles, R.B., Davis, C.E., Coates, R.M. J. Org. Chem. (2006) [Pubmed]
  19. Total synthesis of a diastereomer of the marine natural product clavosolide A. Barry, C.S., Bushby, N., Charmant, J.P., Elsworth, J.D., Harding, J.R., Willis, C.L. Chem. Commun. (Camb.) (2005) [Pubmed]
  20. Synthesis of nopol over MCM-41 catalysts. Villa de P, A.L., Alarcón, E., Montes de Correa, C. Chem. Commun. (Camb.) (2002) [Pubmed]
  21. Synthesis of 3,4-disubstituted piperidines by carbonyl ene and Prins cyclizations: a switch in diastereoselectivity between Lewis and Brønsted acid catalysts. Williams, J.T., Bahia, P.S., Snaith, J.S. Org. Lett. (2002) [Pubmed]
  22. Mesoporous iron phosphate as an active, selective and recyclable catalyst for the synthesis of nopol by Prins condensation. Pillai, U.R., Sahle-Demessie, E. Chem. Commun. (Camb.) (2004) [Pubmed]
  23. Primed in situ labeling (PRINS) for evaluation of gene deletions in cancer. Tharapel, S.A., Kadandale, J.S. Am. J. Med. Genet. (2002) [Pubmed]
  24. Prader Willi/Angelman and DiGeorge/velocardiofacial syndrome deletions: diagnosis by primed in situ labeling (PRINS). Tharapel, A.T., Kadandale, J.S., Martens, P.R., Wachtel, S.S., Wilroy, R.S. Am. J. Med. Genet. (2002) [Pubmed]
  25. Chromosomal localization of single copy genes SRY and SOX3 by primed in situ labeling (PRINS). Kadandale, J.S., Tunca, Y., Tharapel, A.T. Microb. Comp. Genomics (2000) [Pubmed]
  26. Physical mapping of SOD1 to bovine chromosome 1. Schmutz, S.M., Cornwell, D., Moker, J.S., Troyer, D.L. Cytogenet. Cell Genet. (1996) [Pubmed]
  27. The labeling efficiency of human telomeres is increased by double-strand PRINS. Yan, J., Chen, B.Z., Bouchard, E.F., Drouin, R. Chromosoma (2004) [Pubmed]
  28. Detection of human papilloma virus (HPV) genomes by the primed in situ (PRINS) labelling technique. Ramael, M., Van Steelandt, H., Stuyven, G., Van Steenkiste, M., Degroote, J. Pathol. Res. Pract. (1999) [Pubmed]
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