The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

CYT1  -  GDP-mannose pyrophosphorylase/ mannose-1...

Arabidopsis thaliana

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of CYT1

  • Infection with either virulent Pseudomonas syringae or Peronospora parasitica resulted in largely reduced bacterial and hyphal growth in the vtc1 mutant in comparison to the wild type [1].

High impact information on CYT1


Chemical compound and disease context of CYT1


Biological context of CYT1

  • These features indicate that CYT1 plays an essential and unique role in plant growth and development and the establishment of normal cell-wall architecture [6].
  • Characteristic aspects of the cyt1 phenotype, including radial swelling and accumulation of callose, can be mimicked with the inhibitor of N-glycosylation, tunicamycin [7].
  • Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis [7].
  • Presumably, therefore, low AsA is causing vtc1 to enter at least some stage(s) of senescence prematurely with an accompanying increase in salicylic acid levels that results in a faster induction of defense responses [1].
  • Consistent with the proposed role for ascorbate in reactive oxygen species detoxification, lipid peroxides are elevated in soz1, but not in wild type following ozone fumigation [8].

Anatomical context of CYT1

  • PCD symptoms such as nuclear chromatin condensation, the presence of multivesicular bodies, and extensive degradation and disorganization of the grana stacks were observed in 8-week-old vtc2 leaves and in 10-week-old vtc1 leaves [9].
  • Consistent with a role for SCD1 in membrane trafficking, secretory vesicles were found to accumulate in cytokinesis-defective scd1 cells [10].

Associations of CYT1 with chemical compounds

  • To determine the molecular basis of these defects, we have cloned the CYT1 gene by a map-based approach and found that it encodes mannose-1-phosphate guanylyltransferase [7].
  • However, the vtc2 mutants produced greater numbers of longer LRs than wild-type or vtc1 plants at all levels of nitrate [9].
  • A weak mutation in the same gene, called vtc1, has previously been identified on the basis of ozone sensitivity due to reduced levels of ascorbic acid [7].
  • Some cyt1 defects can be partially phenocopied by treatment with the herbicide dichlobenil, which is thought to interfere with cellulose biosynthesis [6].
  • The vtc1 defect does not appear to be the result of a deficiency in L-galactono-1,4-lactone dehydrogenase, an enzyme able to convert L-galactono-1,4-lactone to ascorbic acid [11].

Other interactions of CYT1

  • In addition, the vtc1 mutant exhibits elevated levels of some senescence-associated gene (SAG) transcripts as well as heightened salicylic acid levels [1].
  • By comparative in situ hybridisation to embryo sections, the 35S promoter yielded, relative to the endogenous KNOLLE promoter, low levels of KNOLLE mRNA accumulation in proliferating cells that were insufficient to rescue cytokinesis-defective knolle mutant embryos [12].

Analytical, diagnostic and therapeutic context of CYT1


  1. The timing of senescence and response to pathogens is altered in the ascorbate-deficient Arabidopsis mutant vitamin c-1. Barth, C., Moeder, W., Klessig, D.F., Conklin, P.L. Plant Physiol. (2004) [Pubmed]
  2. The cytokinesis gene KEULE encodes a Sec1 protein that binds the syntaxin KNOLLE. Assaad, F.F., Huet, Y., Mayer, U., Jürgens, G. J. Cell Biol. (2001) [Pubmed]
  3. The Arabidopsis microtubule-associated protein AtMAP65-1: molecular analysis of its microtubule bundling activity. Smertenko, A.P., Chang, H.Y., Wagner, V., Kaloriti, D., Fenyk, S., Sonobe, S., Lloyd, C., Hauser, M.T., Hussey, P.J. Plant Cell (2004) [Pubmed]
  4. Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. Pastori, G.M., Kiddle, G., Antoniw, J., Bernard, S., Veljovic-Jovanovic, S., Verrier, P.J., Noctor, G., Foyer, C.H. Plant Cell (2003) [Pubmed]
  5. Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis. Pavet, V., Olmos, E., Kiddle, G., Mowla, S., Kumar, S., Antoniw, J., Alvarez, M.E., Foyer, C.H. Plant Physiol. (2005) [Pubmed]
  6. A cytokinesis-defective mutant of Arabidopsis (cyt1) characterized by embryonic lethality, incomplete cell walls, and excessive callose accumulation. Nickle, T.C., Meinke, D.W. Plant J. (1998) [Pubmed]
  7. Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis. Lukowitz, W., Nickle, T.C., Meinke, D.W., Last, R.L., Conklin, P.L., Somerville, C.R. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  8. Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant. Conklin, P.L., Williams, E.H., Last, R.L. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  9. Modulation of plant morphology, root architecture, and cell structure by low vitamin C in Arabidopsis thaliana. Olmos, E., Kiddle, G., Pellny, T., Kumar, S., Foyer, C.h. J. Exp. Bot. (2006) [Pubmed]
  10. SCD1 is required for cytokinesis and polarized cell expansion in Arabidopsis thaliana [corrected]. Falbel, T.G., Koch, L.M., Nadeau, J.A., Segui-Simarro, J.M., Sack, F.D., Bednarek, S.Y. Development (2003) [Pubmed]
  11. L-ascorbic acid metabolism in the ascorbate-deficient arabidopsis mutant vtc1. Conklin, P.L., Pallanca, J.E., Last, R.L., Smirnoff, N. Plant Physiol. (1997) [Pubmed]
  12. Cell cycle-independent expression of the Arabidopsis cytokinesis-specific syntaxin KNOLLE results in mistargeting to the plasma membrane and is not sufficient for cytokinesis. Völker, A., Stierhof, Y.D., Jürgens, G. J. Cell. Sci. (2001) [Pubmed]
  13. Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. Conklin, P.L., Norris, S.R., Wheeler, G.L., Williams, E.H., Smirnoff, N., Last, R.L. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  14. Characterization of a cytokinesis defective (cyd1) mutant of Arabidopsis. Yang, M., Nadeau, J.A., Zhao, L., Sack, F.D. J. Exp. Bot. (1999) [Pubmed]
WikiGenes - Universities