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Gene Review

ARG3  -  ornithine carbamoyltransferase

Saccharomyces cerevisiae S288c

Synonyms: J0924, OTCase, Ornithine carbamoyltransferase, Ornithine transcarbamylase, YJL088W
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Disease relevance of ARG3

  • Yeast synthesizes the surface antigen protein of Hepatitis B virus when the structural gene is fused to the promoter from the ARG3 gene [1].
  • The Escherichia coli lacZ gene, was truncated of the eight amino-terminal codons substituted in vitro, on a 2mu plasmid, for the carboxy-terminal and 3'-flanking regions of arg3, leaving only the first 19 proximal codons and approximately 1600 nucleotides of the region preceding arg3 on the yeast chromosome [2].
  • Ornithine transcarbamylase from Salmonella typhimurium: purification, subunit composition, kinetic analysis, and immunological cross-reactivity [3].
  • The activity of OTCase from Bacillus subtilis was partially inhibited by antibodies against the enzyme from S. typhimurium, indicating unusual conservation of primary structure among widely different taxonomic groups [3].
  • In contrast with the dodecameric catabolic OTCase of Pseudomonas aeruginosa, P. furiosus OTCase exhibits no substrate cooperativity [4].

High impact information on ARG3


Biological context of ARG3

  • The involvement of regulatory proteins displaying DNA-binding features and the location of an operator region between the TATA box and the transcription start of the structural gene ARG3 suggest that this mechanism operates at the level of transcription [7].
  • General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene [8].
  • In this work, we have analyzed the time course of the incorporation of radioactive precursors into ARG1 and ARG3 mRNAs and the kinetics of their decay under different regulatory statuses [7].
  • The HPR5 gene, which is nonessential, is tightly linked to the ARG3 locus chromosome X. The hpr5-1 allele contains missense mutation in the putative ATP binding domain [9].
  • Similar sequences situated upstream of ARG5,6 and ARG3 and reported to negatively regulate their expression are able to functionally substitute for the CAR1 UASI elements and mediate reporter gene expression [10].

Anatomical context of ARG3

  • As was previously observed with mammalian mitochondria, this precursor failed both to reach the matrix compartment and to be proteolytically processed; it also failed to exhibit OTCase enzymatic activity [6].
  • When yeast cells containing these species were lysed and fractionated, the OTCase precursor was found to be associated with mitochondrial membranes, while the mature subunit was found partly with mitochondrial membranes and partly in the soluble mitochondrial matrix-containing fraction [6].

Associations of ARG3 with chemical compounds


Physical interactions of ARG3


Other interactions of ARG3

  • Similarly we have measured the mRNA levels for two genes subject to the arginine specific regulation: ARG3 and CAR1, the former gene belongs to the arginine anabolic pathway and the latter to the arginine catabolic one [14].
  • We have measured the mRNA levels for four genes subject to the general amino acid control: HIS4, ARG3, ARG4 and CPAII and compared them to the corresponding enzyme levels [14].
  • The complete nucleotide sequence of the ARG3 structural gene encoding the monomer of the trimeric ornithine carbamoyltransferase (OTCase) (EC has been determined [15].

Analytical, diagnostic and therapeutic context of ARG3


  1. Production in yeast of hepatitis B surface antigen by R-DNA technology. De Wilde, M., Cabezon, T., Harford, N., Rutgers, T., Simoen, E., Van Wijnendaele, F. Dev. Biol. Stand. (1985) [Pubmed]
  2. The promoter region of the arg3 gene in Saccharomyces cerevisiae: nucleotide sequence and regulation in an arg3-lacZ gene fusion. Crabeel, M., Huygen, R., Cunin, R., Glansdorff, N. EMBO J. (1983) [Pubmed]
  3. Ornithine transcarbamylase from Salmonella typhimurium: purification, subunit composition, kinetic analysis, and immunological cross-reactivity. Abdelal, A.T., Kennedy, E.H., Nainan, O. J. Bacteriol. (1977) [Pubmed]
  4. Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus. Legrain, C., Villeret, V., Roovers, M., Gigot, D., Dideberg, O., Piérard, A., Glansdorff, N. Eur. J. Biochem. (1997) [Pubmed]
  5. Purified presequence binding factor (PBF) forms an import-competent complex with a purified mitochondrial precursor protein. Murakami, K., Mori, M. EMBO J. (1990) [Pubmed]
  6. Import and processing of human ornithine transcarbamoylase precursor by mitochondria from Saccharomyces cerevisiae. Cheng, M.Y., Pollock, R.A., Hendrick, J.P., Horwich, A.L. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  7. Arginine-specific repression in Saccharomyces cerevisiae: kinetic data on ARG1 and ARG3 mRNA transcription and stability support a transcriptional control mechanism. Crabeel, M., Lavalle, R., Glansdorff, N. Mol. Cell. Biol. (1990) [Pubmed]
  8. General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene. Crabeel, M., Huygen, R., Verschueren, K., Messenguy, F., Tinel, K., Cunin, R., Glansdorff, N. Mol. Cell. Biol. (1985) [Pubmed]
  9. The hyper-gene conversion hpr5-1 mutation of Saccharomyces cerevisiae is an allele of the SRS2/RADH gene. Rong, L., Palladino, F., Aguilera, A., Klein, H.L. Genetics (1991) [Pubmed]
  10. Tripartite structure of the Saccharomyces cerevisiae arginase (CAR1) gene inducer-responsive upstream activation sequence. Viljoen, M., Kovari, L.Z., Kovari, I.A., Park, H.D., van Vuuren, H.J., Cooper, T.G. J. Bacteriol. (1992) [Pubmed]
  11. Cloning and sequencing of arg3 and arg11 genes of Schizosaccharomyces pombe on a 10-kb DNA fragment. Heterologous expression and mitochondrial targeting of their translation products. Van Huffel, C., Dubois, E., Messenguy, F. Eur. J. Biochem. (1992) [Pubmed]
  12. Yeast epiarginase regulation, an enzyme-enzyme activity control: identification of residues of ornithine carbamoyltransferase and arginase responsible for enzyme catalytic and regulatory activities. El Alami, M., Dubois, E., Oudjama, Y., Tricot, C., Wouters, J., Stalon, V., Messenguy, F. J. Biol. Chem. (2003) [Pubmed]
  13. L-Ornithine carbamoyltransferase from Saccharomyces cerevisiae: steady-state kinetic analysis. Simon, J.P., Stalon, V. Eur. J. Biochem. (1977) [Pubmed]
  14. Participation of transcriptional and post-transcriptional regulatory mechanisms in the control of arginine metabolism in yeast. Messenguy, F., Dubois, E. Mol. Gen. Genet. (1983) [Pubmed]
  15. Nucleotide sequence of the ARG3 gene of the yeast Saccharomyces cerevisiae encoding ornithine carbamoyltransferase. Comparison with other carbamoyltransferases. Huygen, R., Crabeel, M., Glansdorff, N. Eur. J. Biochem. (1987) [Pubmed]
  16. Arginine repression of the Saccharomyces cerevisiae ARG1 gene. Comparison of the ARG1 and ARG3 control regions. Crabeel, M., Seneca, S., Devos, K., Glansdorff, N. Curr. Genet. (1988) [Pubmed]
  17. Regulation of arginine metabolism in Saccharomyces cerevisiae. Association of arginase and ornithine transcarbamoylase. Eisenstein, E., Duong, L.T., Ornberg, R.L., Osborne, J.C., Hensley, P. J. Biol. Chem. (1986) [Pubmed]
  18. Intracellular localization of Aspergillus nidulans ornithine carbamoyltransferase in native host cells and in Saccharomyces cerevisiae cells harbouring its cloned structural gene. Maleszka, R., Dmochowska, A., Zaborowska, D., Cybis, J., Wegleński, P. Acta Biochim. Pol. (1986) [Pubmed]
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