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Gene Review

RTCA  -  RNA 3'-terminal phosphate cyclase

Homo sapiens

Synonyms: RNA cyclase, RNA terminal phosphate cyclase domain-containing protein 1, RNA-3'-phosphate cyclase, RPC, RPC1, ...
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Disease relevance of RTCD1


High impact information on RTCD1


Biological context of RTCD1

  • HILIC/CEC was able to take advantage of very subtle changes in environment around the acetylation sites and thus effect a separation of these analogues not achievable by RPC or CEC alone [10].
  • We identified several potential sigma54-dependent promoters upstream of genes and operons including two homologues of csrA, which encodes the global regulator CsrA, and rtcBA, encoding a RNA 3'-terminal phosphate cyclase [11].
  • With a mean follow-up of 10 months, there was no significant long-term complication.CONCLUSION: In summary, laparoscopic-assisted liver resection is a feasible and safe treatment option for various liver pathologies, such as RPC and primary and secondary liver tumors [12].
  • When the results of bile cultures of RPC patients, admitted to Queen Mary Hospital in 1973-74 and 1979-80, were analysed together with data obtained from the publication of previous investigators, a change in the bacteriology of the disease was noted [13].

Anatomical context of RTCD1


Associations of RTCD1 with chemical compounds

  • The purification procedure involved Triton X-114 extraction, DEAE-Sepharose ion-exchange chromatography and reverse-phase chromatography on a Resource RPC column [18].
  • The separation of the compounds in the crude extract of NA was performed by fast protein liquid chromatography (FPLC) on a Superdex 200 gel-filtration column and by reverse-phase high-pressure liquid chromatography (RPHPLC) on an micro RPC C2/C18 SC2.1/10 column connected to a SMART system [19].
  • The rhVEGF165 had a high affinity for heparin and this characteristic was used to purify this form to homogeneity by heparin affinity, Resource S and Resource RPC columns [20].
  • Cytosolic and nuclear progesterone receptor levels (RPC, RPN) were measured in normal and in pathological human endometria after single-dose progesterone injection 1-3 h before tissue collection (test subjects) and the results compared to those obtained in controls (no progesterone treatment) [15].
  • We have designed an efficient single step purification process using Resource RPC column in conjunction with triethyl amine/acetonitrile solvent system at similar pH at which the in vitro assembly of insulin is carried out from its two chains [21].

Analytical, diagnostic and therapeutic context of RTCD1

  • However, in this case ligation is preceded by an ATP-dependent conversion of the 3'-terminal phosphate to the 2',3' cyclic form by a novel activity, RNA 3'-terminal phosphate cyclase [8].
  • Morbidity was greater after RPC with subsequent ileostomy closure (median hospital stay, 24 versus 11 days; complications, 60 versus 21 per cent; reoperation, 29 versus 3 per cent; return to normal activity; 31 versus 14 weeks) [4].
  • Further purification of TI-9 and CPI-9 was achieved by using reversed-phase HPLC on a PEP RPC column [22].
  • These are based on differences of proteins in size (size-exclusion chromatography, SEC), electrostatic interaction (ion-exchange chromatography, IEC), bioaffinity (bioaffinity chromatography, BAC) and hydrophobic interaction (reversed-phase chromatography, RPC, and hydrophobic-interaction chromatography, HIC) [23].
  • These peptides were then separated by RPC and further analyzed using ESI-MS/MS and MALDI-MS/MS [24].


  1. Induction of human immunoglobulin synthesis and secretion in somatic cell hybrids of mouse myeloma and human B lymphocytes from patients with agammaglobulinemia. Schwaber, J.F., Rosen, F.S. J. Exp. Med. (1978) [Pubmed]
  2. Characterization of the adenylation site in the RNA 3'-terminal phosphate cyclase from Escherichia coli. Billy, E., Hess, D., Hofsteenge, J., Filipowicz, W. J. Biol. Chem. (1999) [Pubmed]
  3. Meningioma. RPC from the AFIP. Huggins, T.J., Ragsdale, B.D., Schnapf, D.J., Madewell, J.E., Youngblood, L. Radiology. (1981) [Pubmed]
  4. Comparison of morbidity and function after colectomy with ileorectal anastomosis or restorative proctocolectomy for familial adenomatous polyposis. Madden, M.V., Neale, K.F., Nicholls, R.J., Landgrebe, J.C., Chapman, P.D., Bussey, H.J., Thomson, J.P. The British journal of surgery. (1991) [Pubmed]
  5. Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis. Billy, E., Wegierski, T., Nasr, F., Filipowicz, W. EMBO J. (2000) [Pubmed]
  6. The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea. Genschik, P., Billy, E., Swianiewicz, M., Filipowicz, W. EMBO J. (1997) [Pubmed]
  7. Cyclization of RNA 3'-terminal phosphate by cyclase from HeLa cells proceeds via formation of N(3')pp(5')A activated intermediate. Filipowicz, W., Strugala, K., Konarska, M., Shatkin, A.J. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  8. RNA 3'-terminal phosphate cyclase activity and RNA ligation in HeLa cell extract. Filipowicz, W., Konarska, M., Gross, H.J., Shatkin, A.J. Nucleic Acids Res. (1983) [Pubmed]
  9. Ascariasis. Khuroo, M.S. Gastroenterol. Clin. North Am. (1996) [Pubmed]
  10. Hydrophilic interaction/cation-exchange chromatography for the purification of synthetic peptides from closely related impurities: serine side-chain acetylated peptides. Litowski, J.R., Semchuk, P.D., Mant, C.T., Hodges, R.S. J. Pept. Res. (1999) [Pubmed]
  11. In silico analysis of the sigma54-dependent enhancer-binding proteins in Pirellula species strain 1. Studholme, D.J., Dixon, R. FEMS Microbiol. Lett. (2004) [Pubmed]
  12. Laparoscopic-assisted liver resection. Tang, C.N., Li, M.K. Journal of hepato-biliary-pancreatic surgery. (2002) [Pubmed]
  13. The changing bacteriology of recurrent pyogenic cholangitis in Hong Kong. Seto, W.H. The Journal of tropical medicine and hygiene. (1985) [Pubmed]
  14. RNA 3'-terminal phosphate cyclase from HeLa cells. Filipowicz, W., Vicente, O. Meth. Enzymol. (1990) [Pubmed]
  15. Progesterone challenge test in postmenopausal women with pathological endometrium. Gorodeski, I.G., Geier, A., Lunenfeld, B., Bahary, C.M. Cancer Invest. (1988) [Pubmed]
  16. Persistence of high CD40 and CD40L expression after restorative proctocolectomy for ulcerative colitis. Polese, L., Angriman, I., Giuseppe, d.e. .F., Cecchetto, A., Sturniolo, G.C., Renata, D., Scarpa, M., Ruffolo, C., Norberto, L., Frego, M., D'Amico, D.F. World J. Gastroenterol. (2005) [Pubmed]
  17. Familial polyposis coli: clinical manifestations, evaluation, management and treatment. Nandakumar, G., Morgan, J.A., Silverberg, D., Steinhagen, R.M. Mt. Sinai J. Med. (2004) [Pubmed]
  18. Assignment of disulfide bridges in bovine CD36. Rasmussen, J.T., Berglund, L., Rasmussen, M.S., Petersen, T.E. Eur. J. Biochem. (1998) [Pubmed]
  19. Stimulatory effects on Na+ transport in renal epithelia induced by extracts of Nigella arvensis are caused by adenosine. Atia, F., Mountian, I., Simaels, J., Waelkens, E., Van Driessche, W. J. Exp. Biol. (2002) [Pubmed]
  20. Expression and characterization of human vascular endothelial growth factor (VEGF165) in insect cells. Lee, G.Y., Jung, W.W., Kang, C.S., Bang, I.S. Protein Expr. Purif. (2006) [Pubmed]
  21. A single-step purification of assembled insulin on a resource RPC column. Babu, K.R., Khanna, N. Prep. Biochem. Biotechnol. (1999) [Pubmed]
  22. Chromatographic separation and partial characterization of microbial low-molecular-weight proteinase inhibitors. Kourteva, I. J. Chromatogr. (1988) [Pubmed]
  23. Column liquid chromatography of integral membrane proteins. Welling, G.W., van der Zee, R., Welling-Wester, S. J. Chromatogr. (1987) [Pubmed]
  24. Targeted glycoproteomics: Serial lectin affinity chromatography in the selection of O-glycosylation sites on proteins from the human blood proteome. Durham, M., Regnier, F.E. Journal of chromatography. A. (2006) [Pubmed]
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