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Gene Review

ldhA  -  D-lactate dehydrogenase

Escherichia coli O157:H7 str. EDL933

 
 
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Disease relevance of ldhA

  • The nucleotide sequence of the D-LDH gene (ldhA) revealed the spontaneous insertion of an E. coli insertion sequence IS2 upstream of the gene coding region [1].
  • Two His residues, His-205 and His-296, in Lactobacillus plantarum D-lactate dehydrogenase are highly conserved in the D-isomer-specific 2-hydroxyacid dehydrogenase family, suggesting that they are candidates for the catalytic His in the enzyme [2].
  • The gene encoding D-lactate dehydrogenase was isolated on a 2.9-kb insert from a library of Pediococcus acidilactici DNA by complementation for growth under anaerobiosis of an Escherichia coli lactate dehydrogenase and pyruvate-formate lyase double mutant [3].
  • 3. Attempts to clone the full-length chromosomal DNA encoding D-lactate dehydrogenase from a partial Sau3AI lambda phage library or an enriched clone bank in E. coli were unsuccessful [4].
  • Purification, properties and DNA sequence of the D-lactate dehydrogenase from Leuconostoc mesenteroides subsp. cremoris [5].
 

High impact information on ldhA

  • d-Lactate dehydrogenase (d-LDH) of Escherichia coli is a peripheral membrane respiratory enzyme involved in electron transfer, located on the cytoplasmic side of the inner membrane. d-LDH catalyzes the oxidation of d-lactate to pyruvate, which is coupled to transmembrane transport of amino acids and sugars [6].
  • Similar values were obtained when the octyl beta-D-glucopyranoside extract was irradiated, and the target size observed for D-lactate dehydrogenase was in good agreement with the molecular size of this enzyme [7].
  • Histidine 296 is essential for the catalysis in Lactobacillus plantarum D-lactate dehydrogenase [2].
  • Nucleotide sequence analysis of the insert DNA showed an exchange of A to G at position 795 resulting in substitution of Glu264 to Gly in the D-lactate dehydrogenase [8].
  • Glutamate 264 modulates the pH dependence of the NAD(+)-dependent D-lactate dehydrogenase [8].
 

Chemical compound and disease context of ldhA

 

Biological context of ldhA

 

Anatomical context of ldhA

 

Associations of ldhA with chemical compounds

 

Other interactions of ldhA

 

Analytical, diagnostic and therapeutic context of ldhA

References

  1. Cloning of the D-lactate dehydrogenase gene from Lactobacillus delbrueckii subsp. bulgaricus by complementation in Escherichia coli. Bernard, N., Ferain, T., Garmyn, D., Hols, P., Delcour, J. FEBS Lett. (1991) [Pubmed]
  2. Histidine 296 is essential for the catalysis in Lactobacillus plantarum D-lactate dehydrogenase. Taguchi, H., Ohta, T. J. Biol. Chem. (1993) [Pubmed]
  3. Pediococcus acidilactici ldhD gene: cloning, nucleotide sequence, and transcriptional analysis. Garmyn, D., Ferain, T., Bernard, N., Hols, P., Delplace, B., Delcour, J. J. Bacteriol. (1995) [Pubmed]
  4. Cloning and overexpression of the Lactobacillus bulgaricus NAD(+)-dependent D-lactate dehydrogenase gene in Escherichia coli:purification and characterization of the recombinant enzyme. Kochhar, S., Chuard, N., Hottinger, H. Biochem. Biophys. Res. Commun. (1992) [Pubmed]
  5. Purification, properties and DNA sequence of the D-lactate dehydrogenase from Leuconostoc mesenteroides subsp. cremoris. Dartois, V., Phalip, V., Schmitt, P., Diviès, C. Res. Microbiol. (1995) [Pubmed]
  6. The crystal structure of D-lactate dehydrogenase, a peripheral membrane respiratory enzyme. Dym, O., Pratt, E.A., Ho, C., Eisenberg, D. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  7. Functional molecular weight of the lac carrier protein from Escherichia coli as studied by radiation inactivation analysis. Goldkorn, T., Rimon, G., Kempner, E.S., Kaback, H.R. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  8. Glutamate 264 modulates the pH dependence of the NAD(+)-dependent D-lactate dehydrogenase. Kochhar, S., Chuard, N., Hottinger, H. J. Biol. Chem. (1992) [Pubmed]
  9. Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae. Skory, C.D. Appl. Environ. Microbiol. (2000) [Pubmed]
  10. Metabolic flux analysis for succinic acid production by recombinant Escherichia coli with amplified malic enzyme activity. Hong, S.H., Lee, S.Y. Biotechnol. Bioeng. (2001) [Pubmed]
  11. Fed-batch two-phase production of alanine by a metabolically engineered Escherichia coli. Smith, G.M., Lee, S.A., Reilly, K.C., Eiteman, M.A., Altman, E. Biotechnol. Lett. (2006) [Pubmed]
  12. Regulation of the ldhA gene, encoding the fermentative lactate dehydrogenase of Escherichia coli. Jiang, G.R., Nikolova, S., Clark, D.P. Microbiology (Reading, Engl.) (2001) [Pubmed]
  13. Site-specific incorporation of 5-fluorotryptophan as a probe of the structure and function of the membrane-bound D-lactate dehydrogenase of Escherichia coli: a 19F nuclear magnetic resonance study. Peersen, O.B., Pratt, E.A., Truong, H.T., Ho, C., Rule, G.S. Biochemistry (1990) [Pubmed]
  14. Characterization of (R)-2-Hydroxyisocaproate Dehydrogenase and a Family III Coenzyme A Transferase Involved in Reduction of L-Leucine to Isocaproate by Clostridium difficile. Kim, J., Darley, D., Selmer, T., Buckel, W. Appl. Environ. Microbiol. (2006) [Pubmed]
  15. D-lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family. Cloning, sequencing, and expression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum. Taguchi, H., Ohta, T. J. Biol. Chem. (1991) [Pubmed]
  16. Immunochemical properties of the membrane-bound D-lactate dehydrogenase from Escherichia coli. Short, S.A., Kaback, H.R., Hawkins, T., Kohn, L.D. J. Biol. Chem. (1975) [Pubmed]
  17. Domain closure, substrate specificity and catalysis of D-lactate dehydrogenase from Lactobacillus bulgaricus. Razeto, A., Kochhar, S., Hottinger, H., Dauter, M., Wilson, K.S., Lamzin, V.S. J. Mol. Biol. (2002) [Pubmed]
  18. Localization of D-lactate dehydrogenase in membrane vesicles prepared by using a french press or ethylenediaminetetraacetate-lysozyme from Escherichia coli. Futai, M., Tanaka, Y. J. Bacteriol. (1975) [Pubmed]
  19. Membrane-bound D-lactate dehydrogenase of Escherichia coli: a model for protein interactions in membranes. Ho, C., Pratt, E.A., Rule, G.S. Biochim. Biophys. Acta (1989) [Pubmed]
  20. Effect of hyperthermia and gamma-radiation on Escherichia coli K1060 D-lactate dehydrogenase. Tamba, M., Simone, G., Yatvin, M.B. Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med. (1984) [Pubmed]
  21. Engineering Escherichia coli to improve culture performance and reduce formation of by-products during recombinant protein production under transient intermittent anaerobic conditions. Lara, A.R., Vazquez-Limón, C., Gosset, G., Bolívar, F., López-Munguía, A., Ramírez, O.T. Biotechnol. Bioeng. (2006) [Pubmed]
  22. Anaerobic fermentation balance of Escherichia coli as observed by in vivo nuclear magnetic resonance spectroscopy. Alam, K.Y., Clark, D.P. J. Bacteriol. (1989) [Pubmed]
  23. Aerobic production of alanine by Escherichia coli aceF ldhA mutants expressing the Bacillus sphaericus alaD gene. Lee, M., Smith, G.M., Eiteman, M.A., Altman, E. Appl. Microbiol. Biotechnol. (2004) [Pubmed]
 
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