Disease relevance of Sinapis

• Seedlings of the white mustard (Sinapis alba L.) are sensitive to the cell-free extracts of a toxigenic strain of Microcystis aeruginosa and to microcystin-LR [1].
• Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa [2].

High impact information on Sinapis

• We have established that a blackspot-resistant species (Sinapis alba) metabolized (14)C-labeled destruxin B to a less toxic product substantially faster than any of the susceptible species [3].
• An in vitro enzyme system for the conversion of amino acid to oxime in the biosynthesis of glucosinolates has been established by the combined use of an improved isolation medium and jasmonic acid-induced etiolated seedlings of Sinapis alba L [4].
• Involvement of cytochrome P450 in oxime production in glucosinolate biosynthesis as demonstrated by an in vitro microsomal enzyme system isolated from jasmonic acid-induced seedlings of Sinapis alba L [4].
• Previous studies using purified RNA polymerase from mustard (Sinapis alba) chloroplasts showed control of transcription by an associated protein kinase [5].
• Cytokinin and gibberellin activate SaMADS A, a gene apparently involved in regulation of the floral transition in Sinapis alba [6].

Biological context of Sinapis

• SaMADS D gene of Sinapis alba was isolated by screening a cDNA library from young inflorescences with a mixture of MADS-box genes of Antirrhinum majus (DEF, GLO, SQUA) as probe [7].
• Transcription factor phosphorylation by a protein kinase associated with chloroplast RNA polymerase from mustard (Sinapis alba) [8].
• Using an antiserum directed against the N-terminal part of Arabidopsis phot1, we show here cross-reaction with phototropin from Avena sativa, Eruca sativa, Glycine max, Lepidium sativum, Lycopersicon esculentum, Pisum sativum, Sinapis alba, and Zea mays [9].
• Hydrolysis of phosphoric acid diesters by snake venom phosphodiesterase and 5'-nucleotide diesters of sinapis alba germs via and covalently enzyme-bound intermediate [10].
• The deduced amino-acid sequence of the protein (designated PnGLP; P.nil germin-like protein) showed homology to that of a GLP of Sinapis alba, a leaf protein, the mRNA accumulation of which showing circadian oscillations [11].

Anatomical context of Sinapis

• Photolyase/blue-light photoreceptor family of proteins includes cyclobutane pyrimidine dimer photolyase, (6-4) photolyase and blue-light photoreceptors that were recently discovered in Arabidopsis thaliana, Sinapis alba and Chlamydomonas reinhardtii [12].

Associations of Sinapis with chemical compounds

• To investigate the regulation of carotenoid biosynthesis during this process at the molecular level, GGPS, PSY and PDS cDNAs have been cloned from white mustard (Sinapis alba L) [13].
• Transcription analysis of a mustard (Sinapis alba L.) serine proteinase inhibitor gene revealed identical 5' termini of mRNAs synthesized during seed maturation and chemical or wounding induction [14].
• In plants of Sinapis alba L. induced to flower by one long day (LD), previous work showed that the phloem sap feeding the shoot apex is enriched in cytokinins of the isopentenyladenine (iP)-type between 9 and 25 h after start of the LD [P. Lejeune et al. (1994) Physiol Plant 90:522-528] [15].
• In seed plants, two different cryptochrome (CRY) genes have been found in Arabidopsis and one in Sinapis, while three genes have been found in the fern Adiantum [16].
• Primary structure of the major allergen of yellow mustard (Sinapis alba L.) seed, Sin a I [17].

Gene context of Sinapis

• The first 500 amino acids exhibit significant homology with previously sequenced DNA photolyases, showing the closest relationship to mustard (Sinapis alba) photolyase (43% identity) [18].
• Glyceraldehyde-3-phosphate dehydrogenase (NADP) from Sinapis alba L. NAD(P)-induced conformation changes of the enzyme [19].
• Two cDNA clones, encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases (GAPDH) from mustard (Sinapis alba), have been identified and sequenced [20].
• This study describes the isolation and purification of cylindrospermopsin from A. ovalisporum with the help of a slightly modified Blue-Green Sinapis Test, a plant test suitable for determining the cyanotoxin content of chromatographic fractions besides plankton samples [21].
• Binding of the trypsin inhibitor from white mustard (Sinapis alba L.) seeds to bovine beta-trypsin: thermodynamic study [22].

Analytical, diagnostic and therapeutic context of Sinapis

• Monomeric cytochrome f has been purified to homogeneity, as judged by polyacrylamide gel electrophoresis, isoelectric focusing and analytical ultracentrifugation, from the leaves of charlock, Sinapis arvensis L [23].
• A biological screening of 146 cultivars of four Triticeae species (Triticum aestivum L., Triticum durum Desf., Triticum spelta L., and Secale cereale L.) demonstrated a high cultivar dependence to suppress the root growth of Sinapis alba L. by root allelopathy in a dose-response bioassay [24].

References