Disease relevance of Thermoplasma

• The homolog from Thermoplasma acidophilum (Ta1419) was overexpressed in E. coli, and the recombinant enzyme was characterized as bifunctional PGI/PMI [1].
• On three enzyme complexes, lumazine synthase from Bacillus subtilis, proteasome from Thermoplasma acidophilum and GTP cyclohydrolase I from Escherichia coli, the decoration sites as determined by electron microscopy (EM) were correlated with their atomic surface structures as obtained by X-ray crystallography [2].
• Polyphenylalanine synthesis with ribosomes and two separated, partially purified elongation factors (EF) was measured in cell-free systems from the archaebacteria Thermoplasma acidophilum and Methanococcus vannielii, in an eukaryotic system from rat liver and an eubacterial one with Escherichia coli ribosomes and factors from Thermus thermophilus [3].

High impact information on Thermoplasma

• The BBS6 protein has similarity to a Thermoplasma acidophilum chaperonin, whereas BBS2 and BBS4 have no significant similarity to chaperonins [4].
• Proteasome from Thermoplasma acidophilum: a threonine protease [5].
• F1 is a 33.5 kDa serine peptidase of the alpha/beta-hydrolase family from the archaeon Thermoplasma acidophilum [6].
• The reaction is based on the oxidation of glucose by Thermoplasma acidophilum glucose dehydrogenase with the concomitant oxidation of NADPH by Pyrococcus furiosus hydrogenase [7].
• The B12-dependent ribonucleotide reductase from the archaebacterium Thermoplasma acidophila: an evolutionary solution to the ribonucleotide reductase conundrum [8].

Biological context of Thermoplasma

• MKKS has sequence homology to the alpha subunit of a prokaryotic chaperonin in the thermosome Thermoplasma acidophilum [9].
• Using a combination of amino acid sequence analysis, three-dimensional crystal structure information, and kinetic analysis, we have characterized TA0175 as phosphoglycolate phosphatase from Thermoplasma acidophilum [10].
• On the basis of sequence and structural homology, we suggest that Ta0583 derives from a ParM-like actin homolog that was once encoded by a plasmid and was transferred into a common ancestor of Thermoplasma and Ferroplasma [11].
• Kinetics and mechanism of the citrate synthase from the thermophilic archaeon Thermoplasma acidophilum [12].
• Amino acid substitutions in the subunit interface enhancing thermostability of Thermoplasma acidophilum citrate synthase [13].

Anatomical context of Thermoplasma

• Recombinant 20S proteasomes from the archaebacterium Thermoplasma acidophilum are imported into nuclei of HeLa and 3T3 cells similar to their eukaryotic counterpart [14].
• Purification and partial characterization of a procaryotic glycoprotein from the plasma membrane of Thermoplasma acidophilum [15].

Associations of Thermoplasma with chemical compounds

• A histone-like protein (HTa) has been isolated from cell extracts of Thermoplasma acidophilum by column chromatography on DNA-cellulose, hydroxylapatite, and Sephadex G-75, HTa elutes from DNA-cellulose in two fractions, one of which contains an 80-residue form of the protein with an NH2-terminal sequence of Val-Gly [16].
• To gain insight into the mechanism of action by LplA, we have determined the crystal structure of Thermoplasma acidophilum LplA in three forms: (i) the apo form; (ii) the ATP complex; and (iii) the lipoyl-AMP complex [17].
• Crystallization and preliminary crystallographic study of glucose dehydrogenase from the archaebacterium Thermoplasma acidophilum [18].
• A ferredoxin from the thermophilic archaebacterium, Thermoplasma acidophilum, is supposed to contain two (4Fe-4S) active centers; one center could be linked by four cysteine residues to the protein and the other bonded with three cysteines and an unknown group [19].
• Malate dehydrogenases from the thermoacidophilic Archaebacteria Thermoplasma acidophilum and Sulfolobus acidocaldarius have been crystallized and characterized by X-ray diffraction measurements [20].

Gene context of Thermoplasma

• Although SCO7095 is distantly related to several proline iminopeptidases, including Thermoplasma acidophilum tricorn-interacting F1, no aminopeptidase activity was detected [21].
• The structure of AF2095 is also similar to the structure of protein TA0108 from archaea Thermoplasma acidophilum, which is deposited in the Protein Data Bank but not functionally annotated [22].
• VAT, the thermoplasma homolog of mammalian p97/VCP, is an N domain-regulated protein unfoldase [23].
• To characterize cytoskeletal components of archaea, the ftsZ gene from Thermoplasma acidophilum was cloned and sequenced [24].
• The crystal structure of citrate synthase from the thermophilic archaeon, Thermoplasma acidophilum [25].

Analytical, diagnostic and therapeutic context of Thermoplasma

• An intracellular serine protease produced by Thermoplasma (Tp.) volcanium was purified using a combination of ammonium sulfate fractionation, ion exchange, and alpha-casein agarose affinity chromatography [26].

References