The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

BFPyV_gp5  -  large T antigen

Budgerigar fledgling disease virus - 1

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of BFPyVgp5


High impact information on BFPyVgp5

  • Cells transformed by Polyoma virus (Py) can undergo a high rate of excision or amplification of integrated viral DNA sequences, and these phenomena require the presence of homology (i.e., repeats) within the viral insertion as well as a functional viral large T antigen (T-Ag) [6].
  • Phosphorylation is not diminished in extracts of polyoma tsA mutant-infected cells shifted to the nonpermissive temperature late in infection, conditions which inactivate the large T antigen [7].
  • By 48 h after infection, almost all (92.8%) bovine corneal endothelial cells expressed large T antigen [5].
  • Bovine and human corneal endothelial cells which expressed large T antigen proliferated and the characteristic morphologic features of corneal endothelium were maintained [5].
  • In contrast, large T antigen alone is sufficient to induce tumors in the endocrine pancreas and thymus [2].

Chemical compound and disease context of BFPyVgp5


Biological context of BFPyVgp5

  • We present evidence that the large T antigen interacts specifically with DNA sequences present in the non-coding region of BFDV; by indirect DNA immunoprecipitation mapping and DNase I footprinting, four regions including 12 DNA-binding sites have been determined that cover most of the BFDV non-coding region [9].
  • Our results are compatible with the hypothesis that large T antigen destabilizes the cellular genome, and that specific mutations arising from this process may contribute to cell immortalization [10].
  • Simian virus 40 (SV40) and polyomavirus (Py) DNA replication require cellular proteins and a virus-encoded early gene product, large T antigen (SVT and PyT, respectively) [11].
  • This recombinant plasmid, pSV01, binds to a purified T antigen in vitro and replicates in monkey cells when supplied with large T antigen [12].
  • Activation of IFN-stimulated gene factor 3 was inhibited in cells derived from a tumor induced by wild-type MPyV but not those from a mutant that lacks the pRB binding site of the large T antigen [13].

Anatomical context of BFPyVgp5

  • SV40 small t antigen which has previously been considered unable to transform cultured cells by itself, was nevertheless able to transform sup+ BHK lines to anchorage independence in the absence of the viral large T antigen [14].
  • Umbilical cord endothelial cells expressing large T antigen: comparison with primary cultures and effect of cell age [15].
  • By using micro-positron-emission tomography, interactions between p53 tumor suppressor and the large T antigen of simian virus 40 were visualized in tumor xenografts of HeLa cells stably transfected with the imaging constructs [16].
  • Infection of primary human prostate epithelial cells with BK polyomavirus dramatically induced Dnmt1 transcription following large T antigen (TAg) translation and E2F activation [17].
  • When grown in 100 microM Zn2+, transformed myocytes expressed the large T antigen, divided rapidly, and acquired an apparently unlimited proliferative capacity [18].

Associations of BFPyVgp5 with chemical compounds

  • The viral large T antigen bound to Janus tyrosine kinase 1 and inactivated signaling through IFN receptors [13].
  • Large T antigen of SV40 interacts with p53, pRb/p107/p130 family members, and the cyclic AMP-responsive element-binding protein (CREB)-binding protein (CBP)/p300 [19].
  • Thus the loss of the growth inhibitory effect of vitamin D3 in HBL100 cells may be caused by the expression of the large T antigen in the cells, and provide further evidence that VDR is required for efficient growth inhibition by vitamin D3 [20].
  • Only 2 cases showed strong/diffuse and moderate/focal staining for LTag with both representing invasive high-grade urothelial carcinoma (where no inclusions were seen on hematoxylin and eosin-stained sections) and both demonstrating positive immunostaining for p53 [21].
  • Sodium butyrate treatment did, however, result in the appearance of a new nuclear protein which bound specifically to a SV40 promoter fragment containing large-T antigen binding sites I and II [22].

Other interactions of BFPyVgp5

  • A recombinant virus containing a genomic copy of the BFDV early region was used for small t antigen expression, and corresponding intron-deleted cDNAs for production of large T antigen derivatives [9].

Analytical, diagnostic and therapeutic context of BFPyVgp5


  1. The genome of budgerigar fledgling disease virus, an avian polyomavirus. Rott, O., Kröger, M., Müller, H., Hobom, G. Virology (1988) [Pubmed]
  2. Role of the amino-terminal domain of simian virus 40 early region in inducing tumors in secretin-expressing cells in transgenic mice. Ratineau, C., Ronco, A., Leiter, A.B. Gastroenterology (2000) [Pubmed]
  3. Interaction of retinoblastoma protein family members with large T-antigen of primate polyomaviruses. White, M.K., Khalili, K. Oncogene (2006) [Pubmed]
  4. T antigens of simian virus 40: molecular chaperones for viral replication and tumorigenesis. Sullivan, C.S., Pipas, J.M. Microbiol. Mol. Biol. Rev. (2002) [Pubmed]
  5. Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro. Feldman, S.T., Gjerset, R., Gately, D., Chien, K.R., Feramisco, J.R. J. Clin. Invest. (1993) [Pubmed]
  6. Amplification and excision of integrated polyoma DNA sequences require a functional origin of replication. Pellegrini, S., Dailey, L., Basilico, C. Cell (1984) [Pubmed]
  7. An activity phosphorylating tyrosine in polyoma T antigen immunoprecipitates. Eckhart, W., Hutchinson, M.A., Hunter, T. Cell (1979) [Pubmed]
  8. Induction and bypass of p53 during productive infection by polyomavirus. Dey, D., Dahl, J., Cho, S., Benjamin, T.L. J. Virol. (2002) [Pubmed]
  9. Expression and DNA binding of budgerigar fledgling disease virus large T antigen. Luo, D., Müller, H., Tang, X.B., Hobom, G. J. Gen. Virol. (1994) [Pubmed]
  10. Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells. Stewart, N., Bacchetti, S. Virology (1991) [Pubmed]
  11. Cell specificity of transcription regulation by papovavirus T antigens and DNA replication. Munholland, J.M., Kelly, J.J., Hassell, J.A., Wildeman, A.G. EMBO J. (1992) [Pubmed]
  12. Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication. Myers, R.M., Tjian, R. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  13. The polyoma virus T antigen interferes with interferon-inducible gene expression. Weihua, X., Ramanujam, S., Lindner, D.J., Kudaravalli, R.D., Freund, R., Kalvakolanu, D.V. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  14. Influence of a hamster tumor suppressor gene on transformation by viral and cellular oncogenes. Benton, B.K., Volpert, O.V., Bouck, N.P. Carcinogenesis (1993) [Pubmed]
  15. Umbilical cord endothelial cells expressing large T antigen: comparison with primary cultures and effect of cell age. Fitzgerald, U., Hettle, S., MacDonald, C., McLean, J.S. In Vitro Cell. Dev. Biol. Anim. (2000) [Pubmed]
  16. Noninvasive imaging of protein-protein interactions in living animals. Luker, G.D., Sharma, V., Pica, C.M., Dahlheimer, J.L., Li, W., Ochesky, J., Ryan, C.E., Piwnica-Worms, H., Piwnica-Worms, D. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  17. Inhibition of DNA methyltransferase activity prevents tumorigenesis in a mouse model of prostate cancer. McCabe, M.T., Low, J.A., Daignault, S., Imperiale, M.J., Wojno, K.J., Day, M.L. Cancer Res. (2006) [Pubmed]
  18. Transfection of human skeletal muscle cells with SV40 large T antigen gene coupled to a metallothionein promoter. Hurko, O., McKee, L., Zuurveld, J.G. Ann. Neurol. (1986) [Pubmed]
  19. Binding of p300/CBP co-activators by polyoma large T antigen. Cho, S., Tian, Y., Benjamin, T.L. J. Biol. Chem. (2001) [Pubmed]
  20. Resistance of HBL100 human breast epithelial cells to vitamin D action. Agadir, A., Lazzaro, G., Zheng, Y., Zhang, X.K., Mehta, R. Carcinogenesis (1999) [Pubmed]
  21. Polyoma virus-associated cellular changes in the urine and bladder biopsy samples: a cytohistologic correlation. Herawi, M., Parwani, A.V., Chan, T., Ali, S.Z., Epstein, J.I. Am. J. Surg. Pathol. (2006) [Pubmed]
  22. Elevation of large-T antigen production by sodium butyrate treatment of SV40-transformed WI-38 fibroblasts. Goldberg, Y.P., Leaner, V.D., Parker, M.I. J. Cell. Biochem. (1992) [Pubmed]
  23. Development and characterization of a conditionally transformed adult human osteoblastic cell line. Bodine, P.V., Trailsmith, M., Komm, B.S. J. Bone Miner. Res. (1996) [Pubmed]
  24. Gene targeting in rat embryo fibroblasts promoted by the polyomavirus large T antigen. Francès, V., Bastin, M. Nucleic Acids Res. (1996) [Pubmed]
  25. Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy. Henson, J., Rosenblum, M., Armstrong, D., Furneaux, H. Neurology (1991) [Pubmed]
  26. Polyomavirus late leader region serves an essential spacer function necessary for viability and late gene expression. Adami, G.R., Carmichael, G.G. J. Virol. (1986) [Pubmed]
  27. Subcellular distribution of simian virus 40 T antigen species in various cell lines: the 56K protein. Luborsky, S.W., Chandrasekaran, K. Int. J. Cancer (1980) [Pubmed]
WikiGenes - Universities