Disease relevance of BK Virus

• This vector includes sequences from BK virus (BKV), the thymidine kinase (TK) gene of herpes simplex virus type 1, and plasmid pML-1 [1].
• To elucidate the basis of the opposing functions and to understand whether and how oncoprotein synthesis in papovaviruses is regulated by p53, we studied modulation of the early promoters of SV40, polyomavirus and BK virus in the absence of the interfering effect of viral large T antigens [2].
• OBJECTIVE: To characterize the T53 cell line and its clones derived from an adenocarcinoma of BK virus (BKV)/tat transgenic mice and to establish the role of native Tat in tumorigenicity, induction of metastases and angiogenesis [3].
• Relationship between the methionine tryptic peptides of simian virus 40 and BK virus tumor antigens [4].
• Transcription of BK virus DNA by Escherichia coli RNA polymerase: size and sequence analysis of RNA [5].

High impact information on BK Virus

• CONCLUSIONS: Testing for BK virus DNA in plasma from renal-allograft recipients with use of the polymerase chain reaction is a sensitive and specific method for identifying viral nephropathy [6].
• Antibody increases to BK virus were associated with a rising seurum creatinine (P = 0.02) and need for transplant biopsy (P = 0.02) [7].
• BK virus (BKV), which infects a large percentage of the human population and has been associated with a variety of human tumors, encodes a TAg homologous to SV40 TAg [8].
• Novel mechanisms of E2F induction by BK virus large-T antigen: requirement of both the pRb-binding and the J domains [8].
• By creating deletion mutants, we determined that the high-level activation of the hybrid BL transcriptional unit by the E1A proteins requires both MLP sequences and an intact BK virus enhancer [9].

Chemical compound and disease context of BK Virus

• In addition, the MLP could be cis-activated by the BK virus enhancer in both human and monkey kidney cells; approximately 20 times more chloramphenicol acetyltransferase was produced from expression vectors containing a hybrid promoter (BL), in which the BK enhancer was upstream of the MLP, than from expression vectors containing the MLP alone [9].
• The relative contributions of cyclophosphamide metabolites and BK virus in the development of HC following BMT are unknown [10].
• The effect of steroid hormones on multiplication of the human polyomavirus BK (BKV) was studied [11].
• An N-linked glycoprotein with alpha(2,3)-linked sialic acid is a receptor for BK virus [12].
• A comparison of the proteins of BK virus and SV40 iodinated with chloramine T before and after disruption in alkaline buffer at pH 10.5 revealed differences between the two viruses in the number and distribution of tyrosines available for iodination [13].

Biological context of BK Virus

• To reflect the high degree of sequence homology between ASV and BK virus, we suggest ASV be renamed BKV(AS) [14].
• Wild-type (wt) BK virus was introduced into permissive BSC-1 cells along with either early or late defective SV40 genomes [15].
• Cross-reaction of BK virus large T antigen with monoclonal antibodies directed against SV40 large T antigen [16].
• DNA sequences for the VP1 gene which codes for the major capsid protein of BK virus (BKV) and may be responsible for antigenic variability were determined for seven BKV isolates [17].
• DNA of the human PLC/PRF/5 hepatoma cell line can induce the appearance of colonies on soft agar after transfection of BK-BK cells (BALB/c mouse kidney cells partially transformed by BK virus) [18].

Anatomical context of BK Virus

• We have monitored BK virus (BKV) antigen expression and multiplication in human monocytes and in a human macrophage (M luminal diameter)-like cell line (U937) in the presence or absence of dilution series of human or rabbit anti-BKV antisera [19].
• Constitutive expression of HIV-1 tat protein in human Jurkat T cells using a BK virus vector [20].

Gene context of BK Virus

• IP-10, Mig and I-TAC were significantly elevated in renal graft recipients with acute rejection, acute tubular injury and BK virus nephritis [21].
• The tryptic peptides of VP1, VP3, VP4, and VP5 combined of SV40 were compared with those of the same polypeptides of BK virus [13].
• This review focuses on polymorphisms in the genomes of non-passaged BK virus isolates from nonneoplastic tissues, with special focus on the transcriptional control region, the regulatory proteins large T-antigen and agnoprotein, and the major capsid protein VP1 [22].
• A monoclonal antibody specific for BK virus large T-antigen (clone BK.T-1) also binds the human Ku autoantigen [23].
• To explore the assembly process, we have compared the structure of two virus-like particles (VLPs) formed, as we found, in a calcium-dependent manner by the VP1 protein of human polyomavirus BK [24].

Analytical, diagnostic and therapeutic context of BK Virus

• Immunohistochemical, in situ hybridization, and polymerase chain reaction studies on paraffin-embedded lung tissue proved BK virus as the etiologic agent of pneumonia [25].
• Antibodies against BK virus in renal transplant recipient sera: results with five different methods indicate frequent reactivations [26].
• INTRODUCTION: Nephropathy associated with the polyomavirus type BK virus (BKV) has emerged as a cause of allograft failure linked to immunosuppressive regimens containing tacrolimus or mycophenolate mofetil (MMF) [27].
• The importance of pre-treatment of human sera for the titration of hemagglutination-inhibiting antibodies towards BK virus [28].
• Polymerase chain reaction (PCR) assays for viral DNA were performed on peripheral blood mononuclear cell specimens to detect infection with HHV-6, HHV-7, HHV-8, cytomegalovirus, Epstein-Barr virus, herpes simplex virus, varicella zoster virus, JC virus, BK virus, and parvovirus B19 [29].

References