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Gene Review

Rbf  -  Retinoblastoma-family protein

Drosophila melanogaster

Synonyms: CG7413, Dmel\CG7413, EG:34F3.3, FBF, RB, ...
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Disease relevance of Rbf


High impact information on Rbf

  • Loss of dE2F or overproduction of its corepressor, RBF, retarded cell proliferation [3].
  • Here we show that Hh signalling promotes transcription of Cyclin E and Cyclin D, two inhibitors of Rb, and principal regulators of the cell cycle during development in Drosophila [4].
  • We identified a mutation in patched (ptc), an inhibitor of Hh signalling, in a genetic screen for regulators of the Retinoblastoma (Rb) pathway in Drosophila [4].
  • We have now identified a binding factor (FBF) which is a cytoplasmic protein that binds specifically to the regulatory region of fem-3 3'UTR and mediates the sperm/oocyte switch [5].
  • The RNA-binding domain of FBF consists of a stretch of eight tandem repeats and two short flanking regions [5].

Chemical compound and disease context of Rbf


Biological context of Rbf


Physical interactions of Rbf

  • Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex [11].
  • In a recent report, Ferres-Marco et al. described how they used the Drosophila eye as a model system and found that elevated Notch signaling in combination with activation of components of the Polycomb complex of transcriptional repressors led to metastatic growth of tumors through epigenetic silencing of the Rbf gene [2].

Regulatory relationships of Rbf

  • Repression of dMyc expression by Wingless promotes Rbf-induced G1 arrest in the presumptive Drosophila wing margin [12].
  • We found that manipulations of endogenous or transgenic NOS activity during imaginal disc development can enhance or suppress the effects of RBF and E2F on development of the eye [13].
  • In mammalian cells, the retinoblastoma protein (Rb) is thought to negatively regulate progression through the G1 phase of the cell cycle by its association with the transcription factor E2F [1-3] [14].

Other interactions of Rbf

  • For some genes the magnitude of this transcriptional derepression is greater in Rbf1 than in E2f2 mutants [8].
  • Instead, dE2F/RBF-mediated repression is exerted on genes that encode an assortment of cellular functions, and these effects are reversed on sets of functionally related genes in particular developmental contexts [7].
  • A Gradient of Epidermal Growth Factor Receptor Signaling Determines the Sensitivity of rbf1 Mutant Cells to E2F-Dependent Apoptosis [15].
  • Here we show that the initial downregulation of expression of the E2f1-target gene RnrS, which occurs during cycles 15 and 16 prior to entry into G1(17), does not require Rbf1 or p27(Dap) [16].
  • These results suggest that the ability of Cyclin D/Cdk4 to drive growth in the proliferating wing cells is distinct from that in the none-dividing eye cells or the ability of activated Ras to induce growth, and that RBF may have a role in regulating growth in the proliferating wing discs [17].

Analytical, diagnostic and therapeutic context of Rbf

  • Here we show by both reverse transcription-PCR and DNA microarray analysis that the transcripts of prereplication complex (pre-RC) genes are elevated compared to the wild type in E2f2, Dp, and Rbf1 mutant follicle cells [8].


  1. Critical role of active repression by E2F and Rb proteins in endoreplication during Drosophila development. Weng, L., Zhu, C., Xu, J., Du, W. EMBO J. (2003) [Pubmed]
  2. Eyeing tumorigenesis: Notch signaling and epigenetic silencing of Rb in Drosophila. Axelson, H. Bioessays (2006) [Pubmed]
  3. Coordination of growth and cell division in the Drosophila wing. Neufeld, T.P., de la Cruz, A.F., Johnston, L.A., Edgar, B.A. Cell (1998) [Pubmed]
  4. Hedgehog regulates cell growth and proliferation by inducing Cyclin D and Cyclin E. Duman-Scheel, M., Weng, L., Xin, S., Du, W. Nature (2002) [Pubmed]
  5. A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line. Zhang, B., Gallegos, M., Puoti, A., Durkin, E., Fields, S., Kimble, J., Wickens, M.P. Nature (1997) [Pubmed]
  6. Cloning and molecular characterization of the Schistosoma mansoni genes RbAp48 and histone H4. Souza, P.P., Santos, D.N., Pena, S.D., Franco, G.R. Mem. Inst. Oswaldo Cruz (2002) [Pubmed]
  7. Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway. Dimova, D.K., Stevaux, O., Frolov, M.V., Dyson, N.J. Genes Dev. (2003) [Pubmed]
  8. Transcriptional repressor functions of Drosophila E2F1 and E2F2 cooperate to inhibit genomic DNA synthesis in ovarian follicle cells. Cayirlioglu, P., Ward, W.O., Silver Key, S.C., Duronio, R.J. Mol. Cell. Biol. (2003) [Pubmed]
  9. Epigenetic silencers and Notch collaborate to promote malignant tumours by Rb silencing. Ferres-Marco, D., Gutierrez-Garcia, I., Vallejo, D.M., Bolivar, J., Gutierrez-Aviño, F.J., Dominguez, M. Nature (2006) [Pubmed]
  10. The role of RBF in the introduction of G1 regulation during Drosophila embryogenesis. Du, W., Dyson, N. EMBO J. (1999) [Pubmed]
  11. Identification of a Drosophila Myb-E2F2/RBF transcriptional repressor complex. Lewis, P.W., Beall, E.L., Fleischer, T.C., Georlette, D., Link, A.J., Botchan, M.R. Genes Dev. (2004) [Pubmed]
  12. Repression of dMyc expression by Wingless promotes Rbf-induced G1 arrest in the presumptive Drosophila wing margin. Duman-Scheel, M., Johnston, L.A., Du, W. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  13. Nitric oxide interacts with the retinoblastoma pathway to control eye development in Drosophila. Kuzin, B., Regulski, M., Stasiv, Y., Scheinker, V., Tully, T., Enikolopov, G. Curr. Biol. (2000) [Pubmed]
  14. S-Phase entry upon ectopic expression of G1 cyclin-dependent kinases in the absence of retinoblastoma protein phosphorylation. Leng, X., Connell-Crowley, L., Goodrich, D., Harper, J.W. Curr. Biol. (1997) [Pubmed]
  15. A Gradient of Epidermal Growth Factor Receptor Signaling Determines the Sensitivity of rbf1 Mutant Cells to E2F-Dependent Apoptosis. Moon, N.S., Di Stefano, L., Dyson, N. Mol. Cell. Biol. (2006) [Pubmed]
  16. Rbf1-independent termination of E2f1-target gene expression during early Drosophila embryogenesis. Shibutani, S., Swanhart, L.M., Duronio, R.J. Development (2007) [Pubmed]
  17. The role of RBF in developmentally regulated cell proliferation in the eye disc and in Cyclin D/Cdk4 induced cellular growth. Xin, S., Weng, L., Xu, J., Du, W. Development (2002) [Pubmed]
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