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Gene Review

ECE1  -  endothelin converting enzyme 1

Sus scrofa

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Disease relevance of ECE1


High impact information on ECE1


Biological context of ECE1

  • Removal of the rhamnose moiety of phosphoramidon (dipeptide 3) reduced the potency for ECE (IC50 = 70 microM), whereas the potencies for NEP (0.003 microM) and ACE (0.20 microM) were increased [4].
  • Subsequent screening of the cDNA library constructed from epithelial cells, a complete cDNA of guinea pig ECE and a partial cDNA encoding preproET-1 were newly isolated [5].

Anatomical context of ECE1


Associations of ECE1 with chemical compounds

  • Class-specific inhibitors of serine, cysteine, and aspartic proteases showed no significant effect on ECE activity in L-GPLGM [9].
  • ECE activity of L-GPLGM was fully inhibited by phosphoramidon, 1,10-phenanthroline, and EDTA [9].
  • A membrane-bound endothelin converting enzyme (ECE) of porcine aortic endothelial cells (ECs) was solubilized with Lubrol PX with high efficiency and stability [10].
  • Metalloendoprotease inhibitors (phosphoramidon and DL-thiorphan), but not captopril, inhibited the contractions elicited by human big ET-1 and big ET-2 but DL-thiorphan was less active, suggesting that a non-selective enzymatic process is involved in conversion of big ET-1 and big ET-2 in addition to a phosphoramidon-sensitive ECE [11].
  • Phosphoramidon-sensitive ECE activity was not enriched on immunomagnetically separated angiotensin-converting enzyme (ACE)-bearing luminal endothelial membranes from pig lung, whereas ACE showed a sevenfold enrichment [12].

Other interactions of ECE1


Analytical, diagnostic and therapeutic context of ECE1


  1. Regional distribution of endothelin-1 and endothelin converting enzyme-1 in porcine endotoxemia. Magder, S., Javeshghani, D., Cernacek, P., Giaid, A. Shock (2001) [Pubmed]
  2. Blockade of endothelin-converting enzyme reduces pulmonary hypertension after cardiopulmonary bypass and circulatory arrest. Kirshbom, P.M., Tsui, S.S., DiBernardo, L.R., Meliones, J.N., Schwinn, D.A., Ungerleider, R.M., Gaynor, J.W. Surgery (1995) [Pubmed]
  3. Metallopeptidase inhibitors induce an up-regulation of endothelin-converting enzyme levels and its redistribution from the plasma membrane to an intracellular compartment. Barnes, K., Shimada, K., Takahashi, M., Tanzawa, K., Turner, A.J. J. Cell. Sci. (1996) [Pubmed]
  4. Differential structure-activity relationships of phosphoramidon analogues for inhibition of three metalloproteases: endothelin-converting enzyme, neutral endopeptidase, and angiotensin-converting enzyme. Kukkola, P.J., Savage, P., Sakane, Y., Berry, J.C., Bilci, N.A., Ghai, R.D., Jeng, A.Y. J. Cardiovasc. Pharmacol. (1995) [Pubmed]
  5. Endothelin-1 production and endothelin converting enzyme expression by guinea pig airway epithelial cells. Shima, H., Yamanouchi, M., Omori, K., Sugiura, M., Kawashima, K., Sato, T. Biochem. Mol. Biol. Int. (1995) [Pubmed]
  6. Expression and function of endothelins, endothelin receptors, and endothelin converting enzyme in the porcine trachea. Yoshimura, H., Nishimura, J., Sakihara, C., Kobayashi, S., Takahashi, S., Kanaide, H. Am. J. Respir. Cell Mol. Biol. (1997) [Pubmed]
  7. ET-1 in the myocardial interstitium: relation to myocyte ECE activity and expression. Ergul, A., Walker, C.A., Goldberg, A., Baicu, S.C., Hendrick, J.W., King, M.K., Spinale, F.G. Am. J. Physiol. Heart Circ. Physiol. (2000) [Pubmed]
  8. Effects of metalloprotease inhibitors on smooth muscle endothelin-converting enzyme activity. Balwierczak, J.L., Kukkola, P.J., Savage, P., Jeng, A.Y. Biochem. Pharmacol. (1995) [Pubmed]
  9. A rapid and selective endothelin-converting enzyme assay: characterization of a phosphoramidon-sensitive enzyme from guinea pig lung membrane. Fawzi, A.B., Cleven, R.M., Wright, D.L. Anal. Biochem. (1994) [Pubmed]
  10. Partial purification of phosphoramidon-sensitive endothelin converting enzyme in porcine aortic endothelial cells: high affinity for Ricinus communis agglutinin. Ohnaka, K., Nishikawa, M., Takayanagi, R., Haji, M., Nawata, H. Biochem. Biophys. Res. Commun. (1992) [Pubmed]
  11. Selective proteolitic activation and degradation of ETs and big ETs in parenchymal strips of the guinea-pig lung. Battistini, B., Brown, M., Vane, J.R. Biochem. Biophys. Res. Commun. (1995) [Pubmed]
  12. Localization and biochemical characterization of endothelin-converting enzyme. Barnes, K., Murphy, L.J., Takahashi, M., Tanzawa, K., Turner, A.J. J. Cardiovasc. Pharmacol. (1995) [Pubmed]
  13. Proteolytic processing of porcine big endothelin-1 catalyzed by cathepsin D. Takaoka, M., Hukumori, Y., Shiragami, K., Ikegawa, R., Matsumura, Y., Morimoto, S. Biochem. Biophys. Res. Commun. (1990) [Pubmed]
  14. Effects of dual endothelin-converting enzyme/neutral endopeptidase inhibitors, CGS 26303 and CGS 26393, on lipopolysaccharide or interleukin-1 beta-stimulated release of endothelin from guinea pig tracheal epithelial cells. Pelletier, S., Battistini, B., Jeng, A.Y., Sirois, P. J. Cardiovasc. Pharmacol. (1998) [Pubmed]
  15. Thromboxane A2-receptor blockade improves cerebral protection for deep hypothermic circulatory arrest. Tsui, S.S., Kirshbom, P.M., Davies, M.J., Jacobs, M.T., Kern, F.H., Gaynor, J.W., Greeley, W.J., Ungerleider, R.M. European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery. (1997) [Pubmed]
  16. Fasting influences steroidogenesis, vascular endothelial growth factor (VEGF) levels and mRNAs expression for VEGF, VEGF receptor type 2 (VEGFR-2), endothelin-1 (ET-1), endothelin receptor type A (ET-A) and endothelin converting enzyme-1 (ECE-1) in newly formed pig corpora lutea. Galeati, G., Forni, M., Spinaci, M., Zannoni, A., Govoni, N., Ribeiro, L.A., Seren, E., Tamanini, C. Domest. Anim. Endocrinol. (2005) [Pubmed]
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